Protection of mice against gastric colonization by Helicobacter pylori by single oral dose immunization with attenuated Salmonella typhimurium producing urease subunits A and B

Gómez‐Duarte, Oscar G.; Lucas, Bernadette; Zhou, Yan; Panthel, Klaus; Haas, Rainer; Meyer, Thomas F.

doi:10.1016/s0264-410x(97)00247-8

Cited by 134 publications

(103 citation statements)

References 54 publications

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“…Lack of protective immune responses after prophylactic vaccination of CD4 1 T-cell-deficient mice C57BL/6 or CD4 1 T-cell-deficient C57BL/6.BM1.GK 1.5 (GK1.5 mice) [21] mice were vaccinated with a single oral dose of recombinant S. Typhimurium expressing H. pylori ureA&B (SL3261 pYZ97) [22], or the carrier (SL3261) and challenged 4 wk later with a single dose of H. pylori Sydney strain 1 (SS1, a mouse-adapted strain) [23]. Three, seven and 21 days after challenge infection, mice were euthanised, stomachs were homogenised and levels of colonisation were determined by quantitative culture and expressed as Log 10 CFU per g tissue.…”

Section: Resultsmentioning

confidence: 99%

“…Female C57BL/6 mice or GK 1.5 [21], (6-8 wk of age) were vaccinated with a single oral dose of 1 Â 10 7 recombinant attenuated Salmonella enterica serovar Typhimurium (S. Typhimurium SL3261pYZ97) [22] (termed 'vaccinated'); control groups were either not vaccinated (termed 'control') or received the carrier strain (SL3261 alone) (termed 'carrier') as previously described [30].…”

Section: Methodsmentioning

confidence: 99%

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Local recall responses in the stomach involving reduced regulation and expanded help mediate vaccine‐induced protection against Helicobacter pylori in mice

Becher¹,

Deutscher²,

Simpfendorfer³

et al. 2010

Eur J Immunol

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Helicobacter pylori is recognised as the chief cause of chronic gastritis, ulcers and gastric cancer in humans. With increased incidence of treatment failure and antibiotic resistance, development of prophylactic or therapeutic vaccination is a desirable alternative. Although the results of vaccination studies in animal models have been promising, studies in human volunteers have revealed problems such as 'post-immunisation gastritis' and comparatively poor responses to vaccine antigens. The focus of this study was to compare the gastric and systemic cellular immune responses induced by recombinant attenuated Salmonella Typhimurium-based vaccination in the C57BL/6 model of H. pylori infection. Analysis of lymphocyte populations in the gastric mucosa, blood, spleen, paragastric LN and MLN revealed that the effects of vaccination were largely confined to the parenchymal stomach rather than lymphoid organs. Vaccine-induced protection was correlated with an augmented local recall response in the gastric mucosa, with increased proportions of CD4 1 T cells, neutrophils and reduced proportions of CD4 1 Treg. CD4 1 T cells isolated from the stomachs of vaccinated mice proliferated ex vivo in response to H. pylori antigen, and secreted Th1 cytokines, particularly IFN-c. This detailed analysis of local gastric immune responses provides insight into the mechanism of vaccine-induced protection.Key words: Helicobacter pylori . Stomach . Treg cells . Vaccine IntroductionHelicobacter pylori is a Gram-negative spiral bacterium that infects the mucous gel layer of the human stomach. Infection is chronic and causes a range of diseases ranging from acute to chronic gastritis, ulcers and, in a small proportion of patients, gastric adenocarcinoma [1].The treatment of H. pylori disease is not straightforward. The most commonly used combination antibiotic therapy, amoxicillin plus clathromycin and a proton pump inhibitor, now only achieve a cure in o80% of cases [2]. This efficacy rate is substantially less than the 495% rate that is deemed acceptable for most other infectious diseases. A prophylactic or therapeutic vaccine would be a cost-effective way to control H. pylori-induced disease.Vaccination is effective in animal models, with 10-to 100-fold reductions (but not sterile immunity) in bacterial colonisation reported in a variety of experimental animals, following immunisation by either oral or parenteral routes (reviewed in [3][4][5]). Oral (mucosal route) vaccination is desirable for a human H. pylori vaccine because of the ease of delivery and, for a live vaccine, the necessity of a single dose is also attractive. 2778with issues such as poor immunogenicity [6], and adverse reactions [7], presenting serious obstacles to further progression of a human vaccine [8]. The reasons for this failure probably result from apparently opposing aspects of the immune response to vaccination. On the one hand vaccination causes 'post-immunisation gastritis' a local inflammatory responses in the stomach in many animal and human recipients, a...

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Local recall responses in the stomach involving reduced regulation and expanded help mediate vaccine‐induced protection against Helicobacter pylori in mice

Becher¹,

Deutscher²,

Simpfendorfer³

et al. 2010

Eur J Immunol

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“…Ab titers were determined colorimetrically by solid-phase ELISA essentially as described (26), except that B. grahamii whole cell lysate was used for Ag coating of microtiter plates. In brief, dilutions of serum samples were added into Ag-coated microtiter plates.…”

Section: Elisamentioning

confidence: 99%

Cutting Edge: Antibody-Mediated Cessation of Hemotropic Infection by the Intraerythrocytic Mouse PathogenBartonella grahamii

Koesling

Aebischer

Falch

et al. 2001

The Journal of Immunology

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The genus Bartonella includes important human-specific and zoonotic pathogens which cause intraerythrocytic bacteremia in their mammalian reservoir host(s). It is accepted that cellular immunity plays a decisive role in the host’s defense against most intracellular bacteria. Bartonella sp. infection in the immunocompetent host typically leads to immunity against homologous challenge. The basis of this immunity, be it cellular or humoral, is unclear. In this study, the course of Bartonella grahamii bacteremia in immunocompetent and immunocompromised mice was compared. In immunocompetent hosts, the bacteremia is transient and induces a strong humoral immune response. In contrast, bacteremia persists in immunocompromised B and T cell-deficient mice. Immune serum transfer beginning with day 6 postinfection to B cell-deficient mice unable to produce Igs converted the persistent bacteremia to a transient course indistinguishable from that of immunocompetent animals. These data demonstrate an essential role for specific Abs in abrogating the intraerythrocytic bacteremia of B. grahamii in mice.

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“…The high global prevalence of H. pylori infection and its certain role in the development of gastric malignancy have encouraged researchers to study different antigens of this bacterium and investigate the possibility of their application as diagnostic indicators and/or vaccines. Overall, different antigens of this bacterium, such as VacA, NAP, Fla, and urease, have been studied for the design of vaccines and diagnostic kits (22)(23)(24)(25)(26)(27).…”

Section: Discussionmentioning

confidence: 99%

Development of an arCagA Antigen-Based Assay for the Detection of Helicobacter pylori in Stool Specimens

Bonyadi

Ghaznavi‐Rad

Mosayebi

et al. 2016

Jundishapur J Microbiol

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Background: Helicobacter pylori infection can lead to the development of gastritis and peptic ulcer in humans. Two categories of diagnostic methods are generally used for the detection of H. pylori infection. Particularly, non-invasive methods are recognized as practical, feasible, and sensitive diagnostic tests for the detection of H. pylori infection. Objectives: This study was designed with the aim to develop an assay, based on cytotoxin-associated gene A (CagA) antigen through western blotting method for the detection of H. pylori in fecal samples. Methods: The antigenic region of CagA (arCagA) gene was amplified by polymerase chain reaction (PCR) method and cloned to pET32a plasmid.

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Protection of mice against gastric colonization by Helicobacter pylori by single oral dose immunization with attenuated Salmonella typhimurium producing urease subunits A and B

Cited by 134 publications

References 54 publications

Local recall responses in the stomach involving reduced regulation and expanded help mediate vaccine‐induced protection against Helicobacter pylori in mice

Local recall responses in the stomach involving reduced regulation and expanded help mediate vaccine‐induced protection against Helicobacter pylori in mice

Cutting Edge: Antibody-Mediated Cessation of Hemotropic Infection by the Intraerythrocytic Mouse PathogenBartonella grahamii

Development of an arCagA Antigen-Based Assay for the Detection of Helicobacter pylori in Stool Specimens

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