Protection of mammalian cell used in biosensors by coating with a polyelectrolyte shell

Germain, Matthieu; Balaguer, Patrick; Nicolas, Jean‐Claude; Lopez, Fréderic; Estève, Jean-Pierre; Sukhorukov, Gleb B.; Winterhalter, Mathias; Richard-Foy, Hélène; Fournier, Didier

doi:10.1016/j.bios.2005.07.011

Cited by 81 publications

(93 citation statements)

References 39 publications

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“…Germain et al 5 developed a cell biosensor by coating genetically-modified MCF7 cells with alternate layers of pDADMAC and PSS. This membrane allows free diffusion of small molecules, excluding proteins (potentially with immunoisolation capability).…”

Section: Direct Pe Coatingmentioning

confidence: 99%

“…Cell microencapsulation is a decades-old concept [1][2][3] that can be developed into many applications, such as cell therapy, 4 cell biosensors, 5 cell immobilization for protein and antibody production, 6 probiotic encapsulation by the food industry or nutraceutics. 7,8 Cell therapy, which is the use of living cells to treat pathological conditions, could be a solution to the difficulties encountered in therapeutic protein delivery.…”

Section: Introductionmentioning

confidence: 99%

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Progress technology in microencapsulation methods for cell therapy

Rabanel

Banquy

Zouaoui

et al. 2009

Biotechnology Progress

122

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Cell encapsulation in microcapsules allows the in situ delivery of secreted proteins to treat different pathological conditions. Spherical microcapsules offer optimal surface-to-volume ratio for protein and nutrient diffusion, and thus, cell viability. This technology permits cell survival along with protein secretion activity upon appropriate host stimuli without the deleterious effects of immunosuppressant drugs. Microcapsules can be classified in 3 categories: matrix-core/shell microcapsules, liquid-core/shell microcapsules, and cells-core/shell microcapsules (or conformal coating). Many preparation techniques using natural or synthetic polymers as well as inorganic compounds have been reported. Matrix-core/shell microcapsules in which cells are hydrogel-embedded, exemplified by alginates capsule, is by far the most studied method. Numerous refinement of the technique have been proposed over the years such as better material characterization and purification, improvements in microbead generation methods, and new microbeads coating techniques. Other approaches, based on liquid-core capsules showed improved protein production and increased cell survival. But aside those more traditional techniques, new techniques are emerging in response to shortcomings of existing methods. More recently, direct cell aggregate coating have been proposed to minimize membrane thickness and implants size. Microcapsule performances are largely dictated by the physicochemical properties of the materials and the preparation techniques employed. Despite numerous promising pre-clinical results, at the present time each methods proposed need further improvements before reaching the clinical phase.

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Section: Direct Pe Coatingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Progress technology in microencapsulation methods for cell therapy

Rabanel

Banquy

Zouaoui

et al. 2009

Biotechnology Progress

122

View full text Add to dashboard Cite

show abstract

“…2,3 It is especially useful in municipal engineering where it is used as a coagulant and flocculant in raw and waste water treatment. 4,5 Therefore, new developments on its preparation, 6,7 application, 8,9 reaction mechanism, [10][11][12] and the relationships between structure and properties [13][14][15][16] have being hot topics all the time. Figure 1(a,b) shows the main structural units of the linear polymer PDMDAAC reported in the literature; Figure 1(c) shows a segment of a pendant double bond that occurs in a low percentage (<3%).…”

Section: Introductionmentioning

confidence: 99%

Preparation and characterization of poly(dimethyldiallylammonium chloride) with high molar mass using high purity industrial monomer

Jia

Zhang

2010

J of Applied Polymer Sci

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A preparation method for high molar mass poly(dimethyldiallylammonium chloride) (PDMDAAC) is reported in this article. PDMDAAC was prepared by using the high purity industrial grade dimethyldiallylammonium chloride (DMDAAC) monomer from one-step method and ammonium persulphate (APS) as the initiator. The initiator was added all at once and the reaction temperature was increased stepwise to complete the polymerization gradually. The effects of several polymerization condition variables on the intrinsic viscosity value ( Under an optimum condition of , polydespersity M w /M n was 2.421, and the R g was 60.3 nm. The structure and properties of products were characterized by FTIR and NMR. Thermal decomposition was determined by TGA-DSC.

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“…The non-pH dependence of PDADMAC provides fully charged polyelectrolytes in a pH range of 1-14. 5 Although enhanced stability is of interest, the overall cell interaction with the film should be as good in the case of PDADMAC and gelatin. 6,7 Meanwhile, several researches have indicated that electrospun nanofibers are very promising as cell scaffolding materials because nanofibrous scaffolds provide a high level of surface area to which cells can attach on account of their three-dimensional feature and high surface area to volume ratio.…”

Section: Introductionmentioning

confidence: 99%

Coating of polyelectrolyte multilayer thin films on nanofibrous scaffolds to improve cell adhesion

Dubas

Kittitheeranun

Rangkupan

et al. 2009

J of Applied Polymer Sci

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The adhesion of L929 cells to poly(e-caprolactone) (PCL) nanofibers was successfully improved via coating with polyelectrolyte multilayer thin films (PEMs), which enhanced the potential of this material as a scaffold in tissue engineering applications. With the electrostatic self-assembly technique, poly(diallyldimethylammonium chloride) (PDADMAC) and poly(sodium 4-styrene sulfonate) (PSS) were formed as four-bilayer PEMs on electrospun PCL nanofiber mats. Because PDADMAC and PSS are strong polyelectrolytes, they provided stable films with good adhesion on the fibers within a wide pH range suitable for the subsequent processes and conditions. PDADMAC and gelatin were also constructed as four-bilayer PEMs on top of the PDADMAC-and PSS-coated nanofibers with the expectation that the gelatin would improve the cell adhesion. L929 cells from mouse fibroblasts were then seeded on both uncoated and coated scaffolds to study the cytocompatibility and in vitro cell behavior. It was revealed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay that both the uncoated and coated nanofiber mats were nontoxic as the cell viability was comparable to that of those cultured in the serum-free medium that was used as a control. The MTT assay also demonstrated that cells proliferated more efficiently on the coated nanofibers than those on the uncoated ones during the 48-h culture period. As observed by scanning electron microscopy, the cells spread well on the coated nanofibers, especially when gelatin was incorporated. The surface modification of PCL nanofiber mats described in this research is therefore an effective technique for improving cell adhesion.

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Protection of mammalian cell used in biosensors by coating with a polyelectrolyte shell

Cited by 81 publications

References 39 publications

Progress technology in microencapsulation methods for cell therapy

Progress technology in microencapsulation methods for cell therapy

Preparation and characterization of poly(dimethyldiallylammonium chloride) with high molar mass using high purity industrial monomer

Coating of polyelectrolyte multilayer thin films on nanofibrous scaffolds to improve cell adhesion

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