Protection of histones isolated from elastase-treated mouse epidermis

Procaccini, Robert L.; Bürki, Kurt; Bresnick, Edward

doi:10.1016/0003-2697(74)90189-4

Cited by 2 publications

(2 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Separations using proteolytic enzytnes such as papain, pronase, collagenases, elastases, trypsin and crude lipase are effective in vivo and //; vitro (15,17). Elastase has been effective and proteolytie degradation of tissue cotnponents can be inhibited by sodiutn bisulfite (9). Proteolytic enzymes such as trypsin and collagenase, both of which are injurious to keratinocyte tnetnbrane proteins (11), may adversely affect drug concentration.…”

Section: Discussionmentioning

confidence: 99%

“…Following epidermal penetration, topically applied compoutids are absorbed by the micro-circulatioti of the dermis (1). Numerous procedures have beeti developed whieh describe effective methods for separating epidermis frotn dermis iti mice (1)(2)(3)(4)(5)(6)(7)(8)(9), rats (10), pigs (11) and humans (12). The purpose of these methods is to produce a clean separation betweeti the epidertnis and dermis and to avoid damage to the underlying dertnis.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Separation of epidermis from dermis in the Rhesus monkey

Frank

Manson

Cartwright

1995

Experimental Dermatology

View full text Add to dashboard Cite

Effective methods exist for separating epidermis from dermis for many species; however, a simple and effective skin separation method for non-human primates is not available. This investigation describes an easy and reliable method for separating epidermis from dermis in Rhesus monkeys. Skin was shaved and washed prior to necropsy. Skin samples were placed on cardboard and then in Whirl-Pak bags, frozen on dry ice and stored at -70 degrees C. Just prior to the separation procedure, Whirl-Pak bags were returned to dry ice storage. Immediately after removal from dry ice, each closed Whirl-Pak bag was placed into a waterbath maintained between 60 and 67 degrees C. After 2 minutes, the Whirl-Pak bag was removed from the waterbath, opened and the skin surface of the application site was gently scraped with a scalpel blade to remove the epidermis. Effectiveness of removal was verified by histologic examination of the remaining dermal samples.

show abstract

Section: Discussionmentioning

confidence: 99%