Protection of Guinea Pigs against Leptospira interrogans Serovar Lai by LipL21 DNA Vaccine

He, Han Jiang; Wang, Wen Yu; Wu, Zhong Dao; Lv, Zhi Yue; Li, Jun; Tan, Li Zhi

doi:10.1038/cmi.2008.48

Cited by 20 publications

(20 citation statements)

References 18 publications

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“…Furthermore, while there was no significant IgG response in the hamsters immunized using the DNA vaccine, a significant number were protected against mortality (62.5%). Several studies have shown the effectiveness of DNA vaccines and reported that protection was associated with a strong antibody response (23)(24)(25). Table 1).…”

Section: Discussionmentioning

confidence: 99%

“…DNA-type vaccine candidates have been evaluated in several leptospirosis vaccine studies (23)(24)(25), including prime-boost strategies (26). In a previous study, we reported that the LemA subunit vaccine preparation induced a strong humoral immune response in immunized mice and that it was recognized by antibodies present in sera of patients with severe leptospirosis (27).…”

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confidence: 99%

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A Prime-Boost Strategy Using the Novel Vaccine Candidate, LemA, Protects Hamsters against Leptospirosis

Hartwig

Forster

Oliveira

et al. 2013

Clin. Vaccine Immunol.

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bToward developing an effective vaccine capable of conferring heterologous protection, the putative lipoprotein LemA, which presents an M3 epitope similar to that of Listeria, was evaluated as a vaccine candidate in the hamster model of leptospirosis. LemA is conserved (>70% pairwise identity) among the pathogenic Leptospira spp., indicating its potential in stimulating a cross-protective immune response. Using different vaccination strategies, including prime-boost, DNA vaccine, and a subunit preparation, recombinant LemA conferred different levels of protection in hamsters. Significant protection against mortality was observed for the prime-boost and the DNA vaccine strategies, which showed 87.5% (P < 0.01) and 62.5% (P < 0.05) efficacy, respectively. Although the subunit vaccine preparation protected 50.0% of immunized hamsters, the level of protection was not significant. None of the hamsters in the control groups survived challenge with a virulent strain of Leptospira interrogans serogroup Icterohaemorrhagiae. Characterization of the immune response found that the strongest antibody response was stimulated by the subunit vaccine preparation, followed by the prime-boost strategy. The DNA vaccine failed to elicit an antibody response in immunized hamsters.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

A Prime-Boost Strategy Using the Novel Vaccine Candidate, LemA, Protects Hamsters against Leptospirosis

Hartwig

Forster

Oliveira

et al. 2013

Clin. Vaccine Immunol.

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“…They play important roles in infection by acting as adhesins, targets of specific antibodies, porins, and receptors for soluble molecules and complement proteins (7,15). A recombinant vaccine based on LipL32 and LipL21 provides partial immunoprotection in a hamster model (17,22). Since the immune responses evoked by whole-cell vaccine or subunit vaccine are often not optimal, epitope-based vaccines provide an alternative strategy.…”

mentioning

confidence: 99%

Identification of Immunodominant B- and T-Cell Combined Epitopes in Outer Membrane Lipoproteins LipL32 and LipL21 of Leptospira interrogans

Lin

Zhao

Qian

et al. 2010

Clin Vaccine Immunol

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Leptospirosis is a serious infectious disease caused by pathogenic Leptospira. B-and T-cell-mediated immune responses contribute to the mechanisms of Leptospira interrogans infection and immune intervention.LipL32 and LipL21 are the conserved outer membrane lipoproteins of L. interrogans and are considered vaccine candidates. In this study, we identified B-and T-cell combined epitopes within LipL32 and LipL21 to further develop a novel vaccine. By using a computer prediction algorithm, two B-and T-cell combined epitopes of LipL21 and four of LipL32 were predicted. All of the predicted epitopes were expressed in a phage display system. Four epitopes, LipL21 residues 97 to 112 and 176 to 184 (LipL21 97-112 and LipL21 176-184 , respectively) and LipL32 133-160 and LipL32 221-247 of LipL32 were selected as antigens by Western blotting and enzymelinked immunosorbent assay. These selected epitopes were also recognized by CD4 ؉ T lymphocytes derived from LipL21-or LipL32-immunized BALB/c (H-2 d ) mice and mainly polarized the immune response toward a Th1 phenotype. The identification of epitopes that have both B-and T-cell immune reactivities is of value for studying the immune mechanisms in response to leptospiral infection and for designing an effective vaccine for leptospirosis.Leptospirosis is a widespread zoonotic disease caused by pathogenic Leptospira spp. (27,36). Patients suffering from leptospirosis have a diverse array of clinical symptoms, such as meningitis (10), pneumonitis (12), hepatitis (1), nephritis (31), and pancreatitis (35), and death may result (5). It is known that zoonotic infections of humans with leptospires are a significant public health problem in developing countries (24).Currently, most vaccines against leptospirosis in animal models have been studied using heat-killed or formalin-killed leptospires, outer membrane proteins, and native or recombinant proteins from leptospires (13,21,23). In general, currently available vaccines made from either inactivated leptospires or their membrane components may elicit immunity but with the disadvantages of incomplete, short-term, limited serovar-specific effects and poor immunological memory (39). In addition, the increasing number of serovars also provides a challenge to developing an ideal vaccine with full protection against pathogenic leptospires.In response to leptospiral infection, the host launches vigorous humoral and cellular immune responses. Evidence indicates that leptospires can activate the immune system, but their immunological effects are still unclear. T lymphocytes play an important role in the recognition and subsequent elimination of tumors and intracellular pathogens. Recent studies have shown that heat-killed Leptospira interrogans induces CD4 ϩ T-cell and ␥/␦ T-cell responses and stimulates type I cytokine production (9, 26). These data suggest that the type I or cellmediated immune response is involved in the protective effect against leptospires.LipL32 and LipL21 are outer membrane proteins of leptospires. They play importa...

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“…5) (Raddi et al 2012). The leptospiral flagellar filaments are similarly complex and consist of a flagellar FlaB core surrounded by a flagellar sheath, presumed to be composed of FlaA (Li et al 2008). The leptospiral flagellar filaments are similarly complex and consist of a flagellar FlaB core surrounded by a flagellar sheath, presumed to be composed of FlaA (Li et al 2008).…”

Section: Leptospiral Flagellar Machinerymentioning

confidence: 99%

Leptospira and Leptospirosis

Faine¹

2015

Current Topics in Microbiology and Immunology

150

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Protection of Guinea Pigs against Leptospira interrogans Serovar Lai by LipL21 DNA Vaccine

Cited by 20 publications

References 18 publications

A Prime-Boost Strategy Using the Novel Vaccine Candidate, LemA, Protects Hamsters against Leptospirosis

A Prime-Boost Strategy Using the Novel Vaccine Candidate, LemA, Protects Hamsters against Leptospirosis

Identification of Immunodominant B- and T-Cell Combined Epitopes in Outer Membrane Lipoproteins LipL32 and LipL21 of Leptospira interrogans

Leptospira and Leptospirosis

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