Protection of epithelial cells from <i>Salmonella enterica</i> serovar Enteritidis invasion by antibodies against the SPI-1 type III secretion system

Desin, Taseen S.; Mickael, Claudia; Lam, Po-King S.; Potter, Andrew; Köster, Wolfgang

doi:10.1139/w10-034

Cited by 13 publications

(9 citation statements)

References 27 publications

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“…Proteins SseB, SipB and SipD were confirmed to be highly immunogenic during the infection. This is in agreement with previous findings [25, 26]. The level of antibodies against these proteins, measured as a density of bands on western blots, was, at least, ten times higher in a group of infected animals than in a group of control or vaccinated animals.…”

Section: Discussionsupporting

confidence: 93%

A proteomic approach to the development of DIVA ELISA distinguishing pigs infected with Salmonella Typhimurium and pigs vaccinated with a Salmonella Typhimurium-based inactivated vaccine

et al. 2016

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Background Salmonella enterica serovar Typhimurium is one of the most common enteropathogenic bacteria found in pigs in Europe. In our previous work, we demonstrated the protective effects in suckling piglets when their dams had been vaccinated with an S. Typhimurium-based inactivated vaccine. This study is focused on a procedure leading to serological discrimination between vaccinated and infected pigs. As we supposed, distinct environment during natural infection and in bacterial cultures used for vaccine preparation led to a slightly different spectrum of expressed S. Typhimurium proteins. The examination of porcine antibodies produced after the experimental infection with S. Typhimurium or after vaccination with S. Typhimurium-based inactivated vaccine by affinity chromatography and mass spectrometry revealed differences in antibody response applicable for serological differentiation of infected from vaccinated animals.ResultsAntibodies against Salmonella SipB, SipD and SseB proteins were detected at much higher levels in post-infection sera in comparison with control and post-vaccination sera. On the other hand, proteins BamB, OppA and a fragment of FliC interacted with antibodies from post-vaccination sera with a much higher intensity than from control and post-infection sera. In addition, we constructed ELISA assays using post-infection antigen - SipB protein and post-vaccination antigen - FliC-fragment and evaluated them on a panel of individual porcine sera.ConclusionsThe analysis of antibody response of infected and vaccinated pigs by proteomic tools enabled to identify S. Typhimurium antigens useful for distinguishing infected from vaccinated animals. This approach can be utilized in other challenges where DIVA vaccine and a subsequent serological assay are required, especially when genetic modification of a vaccine strain is not desirable.Electronic supplementary materialThe online version of this article (doi:10.1186/s12917-016-0879-1) contains supplementary material, which is available to authorized users.

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Section: Discussionsupporting

confidence: 93%

A proteomic approach to the development of DIVA ELISA distinguishing pigs infected with Salmonella Typhimurium and pigs vaccinated with a Salmonella Typhimurium-based inactivated vaccine

et al. 2016

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“…Some of the main variability associated with host specificity is with the secreted effectors and the SipD T3SS tip protein rather than other structural components of the respective T3SSs (206,236). The SPI-1 T3SS tip protein SipD is important for cell invasion; studies showed that antibodies directed at SipD block the ability of S. Enteritidis to invade intestinal epithelial cells (239). Effectors secreted by the SPI-I T3SS may also play a role in host specificity/range; sequence variability in the genes encoding the effector proteins SipA, SopA, and SopE and the chaperones SicP and InvB showed close evolutionary similarity in S. Enteritidis and S. Gallinarum compared to other serovars not as closely associated with avian sources (206).…”

Section: Examples Of Salmonella Host Range In Chicken-related Serovarsmentioning

confidence: 99%

Salmonella Pathogenicity and Host Adaptation in Chicken-Associated Serovars

Foley

Johnson

Ricke

et al. 2013

Microbiol Mol Biol Rev

263

218

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SUMMARY Enteric pathogens such as Salmonella enterica cause significant morbidity and mortality. S. enterica serovars are a diverse group of pathogens that have evolved to survive in a wide range of environments and across multiple hosts. S. enterica serovars such as S . Typhi, S . Dublin, and S . Gallinarum have a restricted host range, in which they are typically associated with one or a few host species, while S . Enteritidis and S . Typhimurium have broad host ranges. This review examines how S. enterica has evolved through adaptation to different host environments, especially as related to the chicken host, and continues to be an important human pathogen. Several factors impact host range, and these include the acquisition of genes via horizontal gene transfer with plasmids, transposons, and phages, which can potentially expand host range, and the loss of genes or their function, which would reduce the range of hosts that the organism can infect. S . Gallinarum, with a limited host range, has a large number of pseudogenes in its genome compared to broader-host-range serovars. S. enterica serovars such as S . Kentucky and S . Heidelberg also often have plasmids that may help them colonize poultry more efficiently. The ability to colonize different hosts also involves interactions with the host's immune system and commensal organisms that are present. Thus, the factors that impact the ability of Salmonella to colonize a particular host species, such as chickens, are complex and multifactorial, involving the host, the pathogen, and extrinsic pressures. It is the interplay of these factors which leads to the differences in host ranges that we observe today.

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“…Anti-tip protein antibodies have been shown to inhibit translocation function in T3SSs for LcrV (Pettersson et al, 1999), PcrV (Sawa et al, 1999), IpaD (Espina et al, 2006;Sani et al, 2007), and SipD (Desin et al, 2010). Antibody binding could directly interfere with tip protein function by blocking interaction with other subunits, with translocator proteins or host receptors.…”

Section: Tip Proteins As Targets Of Protective Antibodiesmentioning

confidence: 99%

Surface organelles assembled by secretion systems of Gram-negative bacteria: diversity in structure and function

2012

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Gram-negative bacteria express a wide variety of organelles on their cell surface. These surface structures may be the end products of secretion systems, such as the hair-like fibers assembled by the chaperone/usher and type IV pilus pathways, which generally function in adhesion to surfaces and bacterial-bacterial and bacterial-host interactions. Alternatively, the surface organelles may be integral components of the secretion machinery itself, such as the needle complex and pilus extensions formed by the type III and type IV secretion systems, which function in the delivery of bacterial effectors inside host cells. Bacterial surface structures perform functions critical for pathogenesis and have evolved to withstand forces exerted by the external environment and cope with defenses mounted by the host immune system. Given their essential roles in pathogenesis and exposed nature, bacterial surface structures also make attractive targets for therapeutic intervention. This review will describe the structure and function of surface organelles assembled by four different Gram-negative bacterial secretion systems: the chaperone/usher pathway, the type IV pilus pathway, and the type III and type IV secretion systems.

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Protection of epithelial cells from Salmonella enterica serovar Enteritidis invasion by antibodies against the SPI-1 type III secretion system

Cited by 13 publications

References 27 publications

A proteomic approach to the development of DIVA ELISA distinguishing pigs infected with Salmonella Typhimurium and pigs vaccinated with a Salmonella Typhimurium-based inactivated vaccine

A proteomic approach to the development of DIVA ELISA distinguishing pigs infected with Salmonella Typhimurium and pigs vaccinated with a Salmonella Typhimurium-based inactivated vaccine

Salmonella Pathogenicity and Host Adaptation in Chicken-Associated Serovars

Surface organelles assembled by secretion systems of Gram-negative bacteria: diversity in structure and function

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