Protection of DNA by metal ions at 95 °C: from lower critical solution temperature (LCST) behavior to coordination-driven self-assembly

Lu, Chang; Xu, Yuancong; Huang, Po‐Jung Jimmy; Zandieh, Mohamad; Wang, Yihao; Zheng, Jinkai; Liu, Juewen

doi:10.1039/d2nr03461a

Cited by 9 publications

(9 citation statements)

References 55 publications

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“…High temperature, ROS, and enzymes are all important factors affecting DNA integrity. Studies have shown that nanospheres formed by metal ions coordinated with DNA could withstand long-term heat treatment [30]. Grass et al also confirmed that silica can resist high temperature and ROS damage to DNA by qPCR analysis [22].…”

Section: Reading Of Encrypted Information In the Si-dna Nanospherementioning

confidence: 98%

“…Fe 2+ ions can self-assemble to form nanoparticles driven by coordination with DNA oligonucleotides [26][27][28]. In addition, other metal ions [29][30][31] can also coordinate with DNA oligonucleotides to form nanoparticles that were used as nano-enzymes [32] with excellent catalytic activities and used as drug carriers [33][34][35] in vivo.…”

Section: Introductionmentioning

confidence: 99%

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Condensed DNA Nanosphere for DNA Origami Cryptography

Gao,

Cai,

Wang

et al. 2023

Chemistry

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Maintaining the confidentiality and integrity of the messages during a transmission is one of the most important aims of encrypted communication systems. Many achievements were made using biomolecules to improve the quality of the messages in communication. At the same time, it is still a challenge to construct cooperative communications based on the interactions between biomolecules to achieve the confidentiality and integrity of the transmitted messages. DNA-based encrypted communications have been developed, and in particular, DNA-origami-based message encryption can combine steganography and pattern encryption and exhibits extremely high confidentiality. Nevertheless, limited by biological characteristics, encrypted messages based on DNA require a strict storage environment in the process of transmission. The integrity of the message encoded in the DNA may be damaged when the DNA is in an unfriendly and hard environment. Therefore, it is particularly significant to improve the stability of DNA when it is exposed to a harsh environment during transmission. Here, we encoded the information into the DNA strands that were condensed for encryption to form a nanosphere covered with a shell of SiO2, which brings high-density messages and exhibits higher stability than separated DNA. The solid shell of SiO2 could prevent DNA from contacting the harsh environment, thereby protecting the DNA structure and maintaining the integrity of the information. At the same time, DNA nanospheres can achieve high throughput input and higher storage density per unit volume, which contribute to confusing the message strand (M-strand) with the interference strand in the stored information. Condensing DNA into the nanosphere that is used for DNA origami cryptography has the potential to be used in harsh conditions with higher confidentiality and integrity for the transmitted messages.

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Section: Reading Of Encrypted Information In the Si-dna Nanospherementioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Condensed DNA Nanosphere for DNA Origami Cryptography

Gao,

Cai,

Wang

et al. 2023

Chemistry

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“…Manganese (Mn) has garnered extensive attention for developing nanozyme due to its low cost, nontoxic nature, and rich valence conversion. − Ligands can regulate the physicochemical properties and catalytic performance of Mn-based nanozymes. − Hence, a suitable ligand is crucial to designing laccase-like nanozymes with good activity and economic applicability. Due to their excellent biocompatibility and abundant binding sites, nucleotides are favorable ligands for self-assembly and coordination with metal ions. − Based on these, Mn–GMPNS was designed and synthesized through a one-pot hydrothermal method. By regulation of the type and ratio of nucleotides, nanozymes with high laccase activity can be screened for the degradation and visual detection of phenolic compounds.…”

Section: Introductionmentioning

confidence: 99%

Multifunctional Manganese–Nucleotide Laccase-Mimicking Nanozyme for Degradation of Organic Pollutants and Visual Assay of Epinephrine via Smartphone

Tang,

Zhou,

Shi

et al. 2024

Anal. Chem.

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As a natural green catalyst, laccase has extensive application in the fields of environmental monitoring and pollutant degradation. However, susceptibility to environmental influences and poor reusability seriously hinder its application. To address these concerns, for the first time, manganese ion replaced copper ion as the active center to coordinate with guanosine monophosphate (GMP) for synthesizing mimic laccase with high catalytic activity. Compared with natural laccase, the laccase-like nanozyme (Mn− GMPNS) demonstrated superior thermal stability, acid−base resistance, salt tolerance, reusability, and substrate universality. Benefiting from the high catalytic activity of Mn−GMPNS, epinephrine, a significant neurotransmitter and hormone associated with numerous diseases, was visually detected within 10 min and a portable assay by smartphone. More encouragingly, Mn−GMPNS can efficiently degrade dye pollutants, achieving a decolorization rate over 70% within 30 min. Thus, the coordination between manganese ion and nucleotide demonstrated the potential in rational design of nanozymes with high catalytic activity, low cost, good stability, and good biocompatibility.

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“…Isothermal enzyme-free amplication methods based on DNA hybridization have been reported as promising for robust ctDNA analysis, such as hybridization chain reaction (HCR), 18,19 DNAzyme catalytic reactions, 20,21 strand-displacement amplication (SDA), 22,23 and catalytic hairpin assembly (CHA). 24,25 Among these approaches, CHA can catalyse the allosteric transition of two metastable DNA hairpins and generate numerous DNA duplexes through toehold-binding and branch migration processes.…”

Section: Introductionmentioning

confidence: 99%

A rapid and highly sensitive ctDNA detection platform based on locked nucleic acid-assisted catalytic hairpin assembly circuits

Tan,

Chen,

Cao

et al. 2023

Anal. Methods

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Protection of DNA by metal ions at 95 °C: from lower critical solution temperature (LCST) behavior to coordination-driven self-assembly

Abstract: While polyvalent metal ions and heating can both degrade nucleic acids, we herein report that a combination of them leads to stabilization. After incubating 4 mM various metal ions and...

Cited by 9 publications

References 55 publications

Condensed DNA Nanosphere for DNA Origami Cryptography

Condensed DNA Nanosphere for DNA Origami Cryptography

Multifunctional Manganese–Nucleotide Laccase-Mimicking Nanozyme for Degradation of Organic Pollutants and Visual Assay of Epinephrine via Smartphone

A rapid and highly sensitive ctDNA detection platform based on locked nucleic acid-assisted catalytic hairpin assembly circuits

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