Protection of Chickens from Newcastle Disease and Infectious Laryngotracheitis with a Recombinant Fowlpox Virus Co-Expressing the F, Hn Genes of Newcastle Disease Virus and GB Gene of Infectious Laryngotracheitis Virus

Sun, Hsin‐Yun; Wang, Yunfeng; Tong, Guangzhi; Zhang, Peijun; DeYuan, Miao; Haidong, Zhi; Wang, Ming; Wang, Mei

doi:10.1637/1933-5334(2008)3[e10:pocfnd]2.0.co;2

Cited by 8 publications

(11 citation statements)

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“…The F and HN proteins of NDV are the virus neutralizing antigens and also are the major protective antigens (9,16,27,60). However, the individual contributions of the F and HN proteins to the serum antibody response and protective immunity were not clearly known.…”

Section: Discussionmentioning

confidence: 99%

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Evaluation of the Newcastle Disease Virus F and HN Proteins in Protective Immunity by Using a Recombinant Avian Paramyxovirus Type 3 Vector in Chickens

Kumar

Nayak

Collins

et al. 2011

J Virol

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Newcastle disease virus (NDV) belongs to serotype 1 of the avian paramyxoviruses (APMV-1) and causes severe disease in chickens. Current live attenuated NDV vaccines are not fully satisfactory. An alternative is to use a viral vector vaccine that infects chickens but does not cause disease. APMV serotype 3 infects a wide variety of avian species but does not cause any apparent disease in chickens. In this study, we constructed a reverse-genetics system for recovery of infectious APMV-3 strain Netherlands from cloned cDNAs. Two recombinant viruses, rAPMV3-F and rAPMV3-HN, were generated expressing the NDV fusion (F) and hemagglutinin-neuraminidase (HN) proteins, respectively, from added genes. These viruses were used to immunize 2-week-old chickens by the oculonasal route in order to evaluate the contribution of each protein to the induction of NDV-specific neutralizing antibodies and protective immunity. Each virus induced high titers of NDV-specific hemagglutination inhibition and serum neutralizing antibodies, but the response to F protein was greater. Protective immunity was evaluated by challenging the immunized birds 21 days later with virulent NDV via the oculonasal, intramuscular, or intravenous route. With oculonasal or intramuscular challenge, all three recombinant viruses (rAPMV3, rAPMV3-F, and rAPMV3-HN) were protective, while all unvaccinated birds succumbed to death. These results indicated that rAPMV3 alone can provide cross-protection against NDV challenge. However, with intravenous challenge, birds immunized with rAPMV3 were not protected, whereas birds immunized with rAPMV3-F alone or in combination with rAPMV3-HN were completely protected, and birds immunized with rAPMV3-HN alone were partially protected. These results indicate that the NDV F and HN proteins are independent neutralization and protective antigens, but the contribution by F is greater. rAMPV3 represents an avirulent vaccine vector that can be used against NDV and other poultry pathogens.

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Section: Discussionmentioning

confidence: 99%

“…Both HN and F glycoproteins are important for virus infectivity and pathogenicity (34,39,40,46). The HN and F proteins produce virus neutralizing antibody responses and are the protective antigens (9,16,27,60). The F protein has been shown to induce protective immunity to NDV in chickens (35).…”

mentioning

confidence: 99%

Evaluation of the Newcastle Disease Virus F and HN Proteins in Protective Immunity by Using a Recombinant Avian Paramyxovirus Type 3 Vector in Chickens

Kumar

Nayak

Collins

et al. 2011

J Virol

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“…Many of these recombinant vaccines are able to induce protective immunity without the problems associated with the re-emergence of latent virus in carrier individuals (Davison et al, 2006;Mebatsion et al, 2008;Sun et al, 2008). Recombinant ILTV strains that lack specific virulence factors have also been developed.…”

Section: Introductionmentioning

confidence: 99%

Comparativein vivosafety and efficacy of a glycoprotein G-deficient candidate vaccine strain of infectious laryngotracheitis virus delivered via eye drop

et al. 2011

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Infectious laryngotracheitis (ILT) is an acute respiratory disease in poultry that is commonly controlled by vaccination with conventionally attenuated virus strains. Despite the use of these vaccines, ILT remains a threat to the intensive poultry industry. Our laboratory has developed a novel candidate vaccine strain of infectious laryngotracheitis virus (ILTV) lacking glycoprotein G (ΔgG-ILTV). The aim of the present study was to directly compare this candidate vaccine with three currently available commercial vaccines in vivo. Five groups of specific-pathogen-free chickens were eye-drop inoculated with one of the three commercial vaccine strains (SA2-ILTV, A20-ILTV or Serva-ILTV), or ΔgG-ILTV, or sterile medium. Vaccine safety was assessed by examining clinical signs, weight gain and persistence of virus in the trachea. Vaccine efficacy was assessed by scoring clinical signs and conducting post-mortem analyses following challenge with virulent virus. Following vaccination, birds that received ΔgG-ILTV had the highest weight gain among the vaccinated groups and had clinical scores that were significantly lower than birds vaccinated with SA2-ILTV or A20-ILTV, but not significantly different from those of birds vaccinated with Serva-ILTV. Analysis of clinical scores, weight gain, tracheal pathology and virus replication after challenge revealed a comparable level of efficacy for all vaccines. Findings from this study further demonstrate the suitability of ΔgG-ILTV as a vaccine to control ILT.

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“…For infectivity and pathogenicity of NDV, both HN and F glycoproteins are important for virus [10] for the production of protective antigens and virus neutralizing antibody responses [11,12]. The F protein has been shown to induce protective immunity to NDV in chickens.…”

Section: Introductionmentioning

confidence: 99%

“…The F protein has been shown to induce protective immunity to NDV in chickens. However, the birds showed lower neutralizing antibody titers, but HN protein has also been shown to protect birds from virulent NDV challenge and it has also been shown that monoclonal antibodies to F protein neutralize NDV better than monoclonal antibodies to HN protein [12].…”

Section: Introductionmentioning

confidence: 99%

Characterization of hemagglutination activity of emerging Newcastle disease virus in Bangladesh

Uddin¹,

Islam²,

Barua³

et al. 2017

Int J One Health

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Aim: Newcastle disease (ND) is an important viral disease for poultry caused by avian paramyxovirus which can be identified by its nature of agglutination activity with red blood cell (RBC) of different species. The study was aimed to characterize the hemagglutinating (HA) activity of ND virus (NDV) at three different temperatures using RBC of five avian species, six mammalian species, and eight different human blood groups. Materials and Methods:The study was conducted from January to December 2014 at Chittagong Veterinary and Animal Sciences University. Five avian and six different mammalian species were selected for the study. In each species, two blood samples were collected aseptically. Eight different blood groups (A+, A−, B+, B−, AB+, AB−, O+, and O−) were studied in human. HA test was performed using two virus strains ND lasota and field isolate of very virulent NDV (VVNDV) with mentioned species of RBC at chilling (4°C), incubating (37°C), and room temperature (24°C).Results: Avian RBC requires less time for agglutination than mammalian RBC. Incubation temperature (37°C) requires lowest time and chilling temperature requires highest time for agglutination of RBC. Duck RBC requires lowest time (17.81 min) while chicken RBC needs highest (57.5 min) time for HA at incubation temperature and at chilling temperature, respectively, against ND lasota virus and with field strain. Goat RBC requires significantly higher time for HA (184.68 min) at chilling temperature than other mammalian species. Human RBC requires almost similar time but O+ and O− blood group do not show any HA activity. Significant variation (p<0.05) found in quail RBC at incubation temperature. In mammalian species, a significant difference (p<0.05) has been observed in goat and horse RBC at chilling; horse and dog RBC at incubation; goat, horse, buffalo, and dog RBC at room temperature. In human, significant variation (p<0.05) has been found in A+, A− and B− blood group in chilling, in B+ blood group at incubation and A+, B+, B−, AB− blood group at room temperature against two virus strains. Conclusion:ND is considered as an economically significant disease which is highly contagious in nature infecting many avian species. The threat of ND outbreak to poultry industry necessitates effective control measures to reduce the burden in commercial and backyard farming in Bangladesh.

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Protection of Chickens from Newcastle Disease and Infectious Laryngotracheitis with a Recombinant Fowlpox Virus Co-Expressing the F, Hn Genes of Newcastle Disease Virus and GB Gene of Infectious Laryngotracheitis Virus

Cited by 8 publications

References 0 publications

Evaluation of the Newcastle Disease Virus F and HN Proteins in Protective Immunity by Using a Recombinant Avian Paramyxovirus Type 3 Vector in Chickens

Evaluation of the Newcastle Disease Virus F and HN Proteins in Protective Immunity by Using a Recombinant Avian Paramyxovirus Type 3 Vector in Chickens

Comparativein vivosafety and efficacy of a glycoprotein G-deficient candidate vaccine strain of infectious laryngotracheitis virus delivered via eye drop

Characterization of hemagglutination activity of emerging Newcastle disease virus in Bangladesh

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