Protection by K+ and Mg2+ of growth and macromolecule synthesis in candicidin-treated yeast

Liras, Paloma; Jo, Lampen

doi:10.1016/0005-2787(74)90359-1

Cited by 18 publications

(12 citation statements)

References 10 publications

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“…The efect ofJil2lpin on the incorporation of phenylalanine into protoplasts of C. albicans The presence of K + and Mg2+ does not protect S. cerevisiae against the action of the polyene antibiotic filipin (Liras & Lampen, 1974). We confirmed these findings with protoplasts of C. albicans.…”

Section: I22 D Kerridge T Y Koh a N D A M Johnsonsupporting

confidence: 69%

“…The fungicidal effects of certain members of this group of antibiotics can be prevented by the addition of K + and Mg2+ to the growth medium (Marini, Arnow & Lampen, 1961 ;Liras & Lampen, 1974). It is assumed that the antibiotic interacts with the plasma membrane rendering it permeable to ions, but the presence of K + and Mg2 + ions in the suspending medium results in maintenance of the internal concentration required for growth.…”

Section: Introductionmentioning

confidence: 99%

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The Interaction of Amphotericin B Methyl Ester with Protoplasts of Candida albicans

Kerridge

Koh

Johnson

1976

Journal of General Microbiology

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The interaction of amphotericin B methyl ester (AME) with protoplasts of Candida albicans was measured indirectly by following the incorporation of [U-l*C]phenylalanine into the acid-insoluble material. The inhibitory effects of AME at the minimum inhibitory concentration were prevented by the addition of 85 mM-KC1 and 45 mM-MgCl,, as shown by Liras & Lampen (1974) for Saccharomyces cerevisiae. In C. albicans, pretreatment of the yeast before antibiotic addition was unnecessary. KCl and MgCl, did not prevent AME from binding to the protoplast membrane. This interaction was reversed by incubating the protoplasts in the presence of the protecting salts.

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Section: I22 D Kerridge T Y Koh a N D A M Johnsonsupporting

confidence: 69%

Section: Introductionmentioning

confidence: 99%

The Interaction of Amphotericin B Methyl Ester with Protoplasts of Candida albicans

Kerridge

Koh

Johnson

1976

Journal of General Microbiology

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“…The protective role of external Mg2`against the antifungal effects of large polyenes has been previously reported (4,7), but in those studies Mg2" did not protect against the action of filipin. The discrepancy between those results and our observations may be due to the different assays applied for the measurements of polyene effects.…”

Section: Resultsmentioning

confidence: 81%

Influence of extracellular K+ or Mg2+ on the stages of the antifungal effects of amphotericin B and filipin

Brajtburg¹,

Medoff²,

Kobayashi³

et al. 1980

Antimicrob Agents Chemother

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The macrolide heptaene amphotericin B (AmB) induced concentration-dependent effects on Saccharomyces cerevisiae which were separable into two distinct stages. At low concentrations the drug inhibited the growth of the yeast and reversibly changed cell permeability to Na+ and K+. At high levels it was lethal. The intracellular K+ concentration of cells with reversible damage (stage I) could be increased by addition of K+ to the medium, but cells irreversibly damaged (stage II) were not able to retain K+. The addition of K+ to the medium did not influence the growth-inhibitory or killing action of AmB. Addition of Mg2" to cultures increased S. cerevisiae resistance to the killing effects of AmB. At low concentrations of AmB, growth inhibition was also decreased by extracellular Mg2+, but at higher concentration of AmB, growth inhibition was increased, probably because the prevention by Mg2`of the lethal effect allowed expression of the inhibitory effect in a greater range. Simultaneous addition of K+ and Mg2" markedly decreased both the inhibitory and lethal action of AmB at all concentrations. Filipin, a pentaene macrolide, had only lethal effects, which were unaffected when K+ was added to the medium but were diminished when medium was supplemented with Mg2+.

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“…2). This protective role of Mg2" against the lethal effects of AmB has also been found in S. cerevisiae (10,26) and C. albicans (23), suggesting that a common mechanism underlies the action of AmB on fungi and parasitic protozoa. In this sense, it is revealing that the inhibitory effect of Mg2I on the action of AmB on heat-transformed leishmanias is much reduced compared with that in nontransformed forms, as indicated by the measured changes in the time lag and Ki for AmBinduced EB incorporation (Fig.…”

Section: Discussionmentioning

confidence: 60%

“…It has generally been assumed that the presence of membrane sterols is a necessary requirement for the formation of AmB channels, leading to the leakage of K+ and essential metabolites and, eventually, to cell death (2,16,24). This hypothesis is supported by experiments which have indicated that the killing effect of AmB on Saccharomyces cerevisiae and Candida albicans is inhibited by the addition of K+ and Mg2" (10,23,26). However, the relationship between the formation of AmB channels through the plasma membrane and the lethal action of AmB on sensitive cells is not yet clear (11).…”

mentioning

confidence: 95%

Enhanced action of amphotericin B on Leishmania mexicana resulting from heat transformation

Ramos

Milhaud

Cohen

et al. 1990

Antimicrob Agents Chemother

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A comparative study of the effect of the polyene antibiotic amphotericin B (AmB) on the viability of Leishmania mexicana promastigotes before and after their transformation by heat into amastigotelike forms was carried out. The kinetics of cell death were followed by spectrofluorometry with the nucleic acid-binding compound ethidium bromide. It was found that the rapid killing effect that is exerted by AmB on Leishmania promastigotes was even faster after their transformation into amastigotelike forms. Binding studies of AmB to Leishmania membranes by circular dichroism indicated that heat transformation modified it from noncooperative to cooperative binding, decreasing the amount of antibiotic that bound to the membranes. Thus, the increased rate of ethidium bromide incorporation into transformed cells was not related either to the amount of AmB bound or to an increased amount of ergosterol in the membrane (the ergosterol/phospholipid ratio was four times smaller after heat shock). An increase in the Mg2e content of the external aqueous solution was able to prevent the AmB-induced incorporation of ethidium bromide into Leishmania promastigotes to a greater extent (Ki = 13.8 mM) than it was into heat-transformed cells (Ki = 64 mM), suggesting that there were significant changes at the Leishmania cell surface on heat transformiltion. The significance of these results for understanding the mechanism of action of AmB on sensitive organisms is discussed.

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Protection by K+ and Mg2+ of growth and macromolecule synthesis in candicidin-treated yeast

Cited by 18 publications

References 10 publications

The Interaction of Amphotericin B Methyl Ester with Protoplasts of Candida albicans

The Interaction of Amphotericin B Methyl Ester with Protoplasts of Candida albicans

Influence of extracellular K+ or Mg2+ on the stages of the antifungal effects of amphotericin B and filipin

Enhanced action of amphotericin B on Leishmania mexicana resulting from heat transformation

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