Protection by Ethanolic Extract from Ulva lactuca L. against Acute Myocardial Infarction: Antioxidant and Antiapoptotic Activities

Widyaningsih, Wahyu; Pramono, Suwijiyo; Zulaela,; Sugiyanto, .; Widyarini, Sitarina

doi:10.21315/mjms2017.24.6.5

Cited by 6 publications

(7 citation statements)

References 26 publications

(20 reference statements)

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“…Induction of apoptosis by DOX was also confirmed biochemically and immunohistochemically by increased DOI: 10.1159/000496109 caspase 3 activity and immunoreaction, respectively, in DOX-treated rats. Consistent with our data, several previous studies also reported a significant increase in caspase 3 activity and protein expression, and downregulation of antiapoptotic proteins following DOX exposure [Soni et al, 2011;El-Agamy et al, 2016;Widyaningsih et al, 2017]. Moreover, ROS initiated by DOX can cause Bax translocation to the outer mitochondrial membrane and subsequently leads to cytochrome C release from the mitochondria into the cardiomyocyte cytoplasm, which further activates caspase 3-dependent apoptosis [Darwish and Abd El Azime, 2013].…”

Section: Discussionsupporting

confidence: 90%

Ameliorative Effect of Cardamom Aqueous Extract on Doxorubicin-Induced Cardiotoxicity in Rats

Gazia¹,

El-Magd²

2018

Cells Tissues Organs

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This study was conducted to evaluate the potential cardioprotective effect of cardamom (CAR) against myocardial injuries induced by doxorubicin (DOX) in rats through investigation of histological alterations and the associated oxidative stress, apoptosis, inflammation, and angiogenesis. This study included 30 adult male albino rats that were randomized to 3 groups (n = 10/group): group I (control), group II (DOX) rats injected with DOX (2.5 mg/kg body weight [BW] i.p.) every other day for 2 weeks, and group III (CAR+DOX) received CAR extract (200 mg/kg BW) orally for 3 weeks, and 1 week later (starting from the 2nd week) they were injected with DOX (2.5 mg/kg BW i.p.) every other day for 2 weeks. Rats treated with DOX alone exhibited notable myocardial damage (discontinuity and disorganization of cardiac muscle fibers, mononuclear cell infiltration, and apparent increases in collagen fiber deposition) accompanied by loss of function (revealed by elevated serum levels of lactate dehydrogenase, creatine kinase, and cardiac troponin), induction of oxidative stress (indicated by higher levels of nitric oxide and malondialdehyde, and lower levels of superoxide dismutase, catalase, and glutathione peroxidase), apoptosis (evidenced by high caspase 3 activity and immunostaining), and inflammation (marked by high cardiac NFκB level). However, administration of CAR not only ameliorated all deleterious effects of DOX but also induced angiogenesis, as indicated by a significant increase in VEGF immunoreactivity. These data indicate that CAR could relieve DOX-induced cardiotoxicity, at least in part, via reductions in oxidative stress, apoptosis, and inflammation and increased tissue regeneration via induction of angiogenesis. Therefore, CAR could be a promising cytoprotective agent against DOX cardiotoxicity.

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Section: Discussionsupporting

confidence: 90%

Ameliorative Effect of Cardamom Aqueous Extract on Doxorubicin-Induced Cardiotoxicity in Rats

Gazia¹,

El-Magd²

2018

Cells Tissues Organs

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“…In this regard, the MCF-7 cells were exposed to 250–1000 μg/mL of KLH for 24 h, and oxidative stress markers (GSH and LPO), ROS generation, MMP, and caspase enzyme activities were studied. ROS raised LPO level is known to decrease endogenous antioxidants such as glutathione (GSH) ( Widyaningsih et al, 2017 ). The elevated level of ROS has been reported to be involved in the cancer cell death ( NavaneethaKrishnan et al, 2019 ).…”

Section: Discussionmentioning

confidence: 99%

Anticancer efficacies of Krameria lappacea extracts against human breast cancer cell line (MCF-7): Role of oxidative stress and ROS generation

Al-Oqail

2021

Saudi Pharmaceutical Journal

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Breast cancer is a growing health issue globally and accounts as a second most cause of mortality. Natural products have been a fundamental of health care for long. Plants derived natural products have gained considerable attention over synthetic medicines, since they are safe and non-toxic. Krameria lappacea (Dombey) Burdet and B.B. Simpson plant belonging to Krameriaceae family, has been known for its beneficial effects against diseases. Herein, firstly, cytotoxic potential of petroleum ether (KLH), chloroform (KLC), ethyl acetate (KLEA), and ethanolic (KLET) extracts of K. lappacea was screened against MCF-7 cells exposed to 10–1000 μg/mL for 24 h. Secondly, the most cytotoxic extract (KLH) was used to explore the mechanisms of cytotoxicity in MCF-7 cells. MCF-7 cells were treated with KLH at 250–1000 μg/mL to measure the oxidative stress markers (glutathione (GSH) and lipid peroxidation (LPO)) and reactive oxygen species (ROS) generation. Further, loss of mitochondrial membrane potential (MMP) and caspase-3 and -9 enzyme activities were studied. The viability of MCF-7 cells were decreased from 44% to 90% for KLH, from 7% to 71% for KLEA, from 39% to 80% for KLC, and from 3% to 81% for KLET, respectively at 250–1000 μg/mL as observed by MTT assay. An increase of 91% in LPO and 2.2-fold in ROS generation and a decrease of 59% in GSH and 68% in MMP levels at 1000 μg/mL showed that KLH induced MCF-7 cell death via oxidative stress and elevated level of ROS generation which further leads to mitochondrial membrane dysfunction and activation of caspase enzymes. The findings of this study provide a mechanistic insight on anticancer efficacies of K. lappacea extracts against MCF-7 cells and support the use of it for the treatment of breast cancer diseases.

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“…Although the cardioprotective nature of melatonin against ISO induced myocardial injury in rats has previously been shown [ 83 ], this new mechanism of dual protection afforded by melatonin to antioxidant enzymes, evident from the ITC pattern between ISO, melatonin and catalase, which shows that melatonin sequesters ISO molecules and prevents it from attacking the binding sites of catalase along with occupying and masking the active sites of catalase enzyme themselves thereby preventing any possibility of interaction between ISO and catalase is unique to this study and highlights the beneficial role of melatonin. Although many medicinal plants with antioxidant properties have been used as a cardioprotective agent against ISO [ 84 ], melatonin remains the most potent and promising candidate of all. The therapeutic role of exogenous melatonin in cardiovascular diseases has been discussed previously [ 85 ] suggesting it to be consumed through plant foods or by direct supplementation to promote various health benefits [ 86 ].…”

Section: Discussionmentioning

confidence: 99%

Activation of SIRT1/PGC 1α/SIRT3 pathway by melatonin provides protection against mitochondrial dysfunction in isoproterenol induced myocardial injury

Naaz

Mishra

Pal

et al. 2020

Heliyon

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Aims Preventing mitochondrial dysfunction and enhancing mitochondrial health and biogenesis is a crucial therapeutic approach to ameliorate injury following acute myocardial infarction. Although the antioxidant role of melatonin against ischemia/reperfusion injury has been reported, the exact mechanism of protection, in vivo , remains poorly understood. This study aims to identify and elaborate upon mechanism of melatonin protection of rat cardiac mitochondria against acute myocardial infarction. Main methods Rats were pre-treated with melatonin (10 mg/kg body weight (b.w.); intraperitoneally, i.p.) before isoproterenol bitartrate (ISO) administration (25 mg/kg body weight (b.w.) subcutaneously,s.c.) and their effect on rat heart mitochondrial structure and function was studied. Biochemical changes in activity of biomarkers of oxidative stress, antioxidant enzymes as well as Krebs' cycle enzymes were analyzed. Gene expression studies and Isothermal titration calorimetric studies with pure catalase and ISO were also carried out. Key findings Melatonin was shown to reduce ISO induced oxidative stress, by stimulating superoxide dismutase activity and removing the inhibition of Krebs' cycle enzymes. Herein we report for the first time in rat model that melatonin activates the SIRT1-PGC-1α-SIRT3 signaling pathways after ISO administration, which ultimately induces mitochondrial biogenesis. Melatonin exhibited significant protection of mitochondrial architecture and topology along with increased calcium ion permeability and reactive oxygen species (ROS) generation induced by ISO. Isothermal calorimetric studies revealed that melatonin binds to ISO molecules and sequesters them from the reaction thereby limiting their interaction with catalase along with occupying the binding sites of catalase themselves. Significance Activation of SIRT1-PGC-1α-SIRT3 pathway by melatonin along with its biophysical properties prevents ISO induced mitochondrial injury in rat heart.

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Protection by Ethanolic Extract from Ulva lactuca L. against Acute Myocardial Infarction: Antioxidant and Antiapoptotic Activities

Cited by 6 publications

References 26 publications

Ameliorative Effect of Cardamom Aqueous Extract on Doxorubicin-Induced Cardiotoxicity in Rats

Ameliorative Effect of Cardamom Aqueous Extract on Doxorubicin-Induced Cardiotoxicity in Rats

Anticancer efficacies of Krameria lappacea extracts against human breast cancer cell line (MCF-7): Role of oxidative stress and ROS generation

Activation of SIRT1/PGC 1α/SIRT3 pathway by melatonin provides protection against mitochondrial dysfunction in isoproterenol induced myocardial injury

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