Protection and antibody isotype responses against Fasciola hepatica with specific antibody to pIII-displayed peptide mimotopes of cathepsin L1 in sheep

Villa-Mancera, Abel; Méndez-Mendoza, Maximino

doi:10.1016/j.tvjl.2012.03.003

Cited by 22 publications

(9 citation statements)

References 31 publications

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“…Thus, cattle vaccinated with a lettuce-based edible vaccine expressing CPFhW were better protected from F. hepatica infection than sheep. The level of protection observed in the present vaccine study was within the range seen in cattle and sheep experiments performed to date with various FhCL1 preparations delivered via standard routes ( 4 , 6 , 23 – 25 ), however, in contrast to the vaccination experiments reported to date, no adjuvants were used during the present vaccination trials.…”

Section: Discussionsupporting

confidence: 76%

A Preliminary Study of a Lettuce-Based Edible Vaccine Expressing the Cysteine Proteinase of Fasciola hepatica for Fasciolosis Control in Livestock

et al. 2018

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Oral vaccination with edible vaccines is one of the most promising approaches in modern vaccinology. Edible vaccines are an alternative to conventional vaccines, which are typically delivered by injection. Here, freeze-dried transgenic lettuce expressing the cysteine proteinase of the trematode Fasciola hepatica (CPFhW) was used to orally vaccinate cattle and sheep against fasciolosis, which is the most important trematode disease due to the parasite's global distribution, wide spectrum of host species and significant economic losses of farmers. In the study, goals such as reducing the intensity of infection, liver damage and F. hepatica fecundity were achieved. Moreover, we demonstrated that the host sex influenced the outcome of infection following vaccination, with female calves and male lambs showing better protection than their counterparts. Since differences occurred following vaccination and infection, different immunization strategies should be considered for different sexes and host species when developing new control methods. The results of the present study highlight the potential of oral vaccination with plant-made and plant-delivered vaccines for F. hepatica infection control.

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Section: Discussionsupporting

confidence: 76%

A Preliminary Study of a Lettuce-Based Edible Vaccine Expressing the Cysteine Proteinase of Fasciola hepatica for Fasciolosis Control in Livestock

et al. 2018

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“…According to reports in the pertinent literature, cysteine proteases released by F. hepatica play a key role in parasite feeding, migration through host tissues and immune evasion and they are considered good vaccine candidates [32]. Cysteine proteases have been used as vaccine candidates in immunisation trials involving experimental models such as cattle and sheep and using different adjuvants, resulting in up to 79% protection [33]–[38]. The rationale behind selecting proteins that could be secreted by F. hepatica has been based on the fact that these proteins are exposed to a host’s immune system, making them easily recognisable.…”

Section: Discussionmentioning

confidence: 99%

In Vitro and In Vivo Studies for Assessing the Immune Response and Protection-Inducing Ability Conferred by Fasciola hepatica-Derived Synthetic Peptides Containing B- and T-Cell Epitopes

et al. 2014

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Fasciolosis is considered the most widespread trematode disease affecting grazing animals around the world; it is currently recognised by the World Health Organisation as an emergent human pathogen. Triclabendazole is still the most effective drug against this disease; however, resistant strains have appeared and developing an effective vaccine against this disease has increasingly become a priority. Several bioinformatics tools were here used for predicting B- and T-cell epitopes according to the available data for Fasciola hepatica protein amino acid sequences. BALB/c mice were immunised with the synthetic peptides by using the ADAD vaccination system and several immune response parameters were measured (antibody titres, cytokine levels, T-cell populations) to evaluate their ability to elicit an immune response. Based on the immunogenicity results so obtained, seven peptides were selected to assess their protection-inducing ability against experimental infection with F. hepatica metacercariae. Twenty-four B- or T-epitope-containing peptides were predicted and chemically synthesised. Immunisation of mice with peptides so-called B1, B2, B5, B6, T14, T15 and T16 induced high levels of total IgG, IgG1 and IgG2a (p<0.05) and a mixed Th1/Th2/Th17/Treg immune response, according to IFN-γ, IL-4, IL-17 and IL-10 levels, accompanied by increased CD62L+ T-cell populations. A high level of protection was obtained in mice vaccinated with peptides B2, B5, B6 and T15 formulated in the ADAD vaccination system with the AA0029 immunomodulator. The bioinformatics approach used in the present study led to the identification of seven peptides as vaccine candidates against the infection caused by Fasciola hepatica (a liver-fluke trematode). However, vaccine efficacy must be evaluated in other host species, including those having veterinary importance.

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“…Using CL1/CL2 mimitopes, Villa‐Mancera et al. () reported 47% protection in sheep, and CL1 mimitopes on their own led to protection of 51% in sheep (Villa‐Mancera & Méndez‐Mendoza, ) and 46%–79% in goats (Villa‐Mancera et al., ). These promising results in sheep and goats have yet to be validated in subsequent trials and in field trials.…”

Section: Vaccine Developmentmentioning

confidence: 99%

Fasciola and fasciolosis in ruminants in Europe: Identifying research needs

Beesley

Caminade

Charlier

et al. 2017

Transbound Emerg Dis

157

111

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Summary Fasciola hepatica is a trematode parasite with a global distribution, which is responsible for considerable disease and production losses in a range of food producing species. It is also identified by WHO as a re‐emerging neglected tropical disease associated with endemic and epidemic outbreaks of disease in human populations. In Europe, F. hepatica is mostly associated with disease in sheep, cattle and goats. This study reviews the most recent advances in our understanding of the transmission, diagnosis, epidemiology and the economic impact of fasciolosis. We also focus on the impact of the spread of resistance to anthelmintics used to control F. hepatica and consider how vaccines might be developed and applied in the context of the immune‐modulation driven by the parasite. Several major research gaps are identified which, when addressed, will contribute to providing focussed and where possible, bespoke, advice for farmers on how to integrate stock management and diagnosis with vaccination and/or targeted treatment to more effectively control the parasite in the face of increasing the prevalence of infection and spread of anthelmintic resistance that are likely to be exacerbated by climate change.

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Protection and antibody isotype responses against Fasciola hepatica with specific antibody to pIII-displayed peptide mimotopes of cathepsin L1 in sheep

Cited by 22 publications

References 31 publications

A Preliminary Study of a Lettuce-Based Edible Vaccine Expressing the Cysteine Proteinase of Fasciola hepatica for Fasciolosis Control in Livestock

A Preliminary Study of a Lettuce-Based Edible Vaccine Expressing the Cysteine Proteinase of Fasciola hepatica for Fasciolosis Control in Livestock

In Vitro and In Vivo Studies for Assessing the Immune Response and Protection-Inducing Ability Conferred by Fasciola hepatica-Derived Synthetic Peptides Containing B- and T-Cell Epitopes

Fasciola and fasciolosis in ruminants in Europe: Identifying research needs

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