“…Oropharyngeal tonsil, mandibular lymph node (LN), parotid and retropharyngeal LN, lung, tracheobronchial LN, mediastinal LN, spleen, ileocecal valve, mesenteric LN, and hepatic LN specimens from 27 BCG-vaccinated wild boar in four different experiments were collected at necropsy (days 175, 189, 258, and 300 p.v.) and cultured in solid and liquid media using the Bactec MGIT system (Becton, Dickinson, Sparks, MD), as described by Garrido et al (34). The isolates resulting from positive cultures were further characterized by spoligotyping (37), which allows identification of Mycobacterium tuberculosis complex strains based on the presence or absence of spacers in the direct repeat region.…”