Protection against Pseudomonas plecoglossicida in Epinephelus coioides immunized with a cspA1-knock-down live attenuated vaccine

Qi, Weilu; Xü, Wei; Zhao, Lingmin; Xu, Xiaojin; Luo, Zengwei; Huang, Lixing; Yan, Qingpi

doi:10.1016/j.fsi.2019.04.029

Cited by 15 publications

(6 citation statements)

References 19 publications

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“…For dual RNA-Seq, spleen specimens from six weight-matched E. coioides after P. plecoglossicida infection were collected at 48 h post-infection (hpi); two consecutive samples were pooled ( 44 ). For bacteria distribution assessment, spleens from 3 E. coioides were obtained at 1, 6, 12, 24, 48, 72 and 96 hpi, respectively ( 45 , 46 ). Spleens and blood specimens from 3 E. coioides were collected at 48 hpi, for Zn concentration measurements by inductively coupled plasma mass spectrometry (ICP-MS).…”

Section: Methodsmentioning

confidence: 99%

The Zinc Nutritional Immunity of Epinephelus coioides Contributes to the Importance of znuC During Pseudomonas plecoglossicida Infection

et al. 2021

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Previously, the dual RNA-seq was carried out in a Pseudomonas plecoglossicida- Epinephelus coioides infection model to investigate the dynamics of pathogen-host interplay in vivo. ZnuC, a member of ZnuCBA Zn importer, was found transcriptionally up-regulated during infection. Thus, this study aimed to assess its role during the trade-off for Zn between host and P. plecoglossicida. ICP-MS analysis and fluorescent staining showed that Zn was withheld from serum and accumulated in the spleen, with increased Zn uptake in the Golgi apparatus of macrophages after infection. Additionally, growth assay, macrophage infection and animal infection after gene knockout / silencing revealed that znuC was necessary for growth in Zn-limiting conditions, colonization, intracellular viability, immune escape and virulence of P. plecoglossicida. Further analysis with dual RNA-seq revealed associations of host’s Zn nutritional immunity genes with bacterial Zn assimilation genes. IL6 and ZIP4 played key roles in this network, and markedly affected znuB expression, intracellular viability and immune escape, as revealed by gene silencing. Moreover, EMSA and GFP reporter gene analysis showed that Fur sensed changes in Fe concentration to regulate znuCBA in P. plecoglossicida. Jointly, these findings suggest a trade-off for Zn between host and P. plecoglossicida, while ZnuC is important for P. plecoglossicida Zn acquisition.

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Section: Methodsmentioning

confidence: 99%

The Zinc Nutritional Immunity of Epinephelus coioides Contributes to the Importance of znuC During Pseudomonas plecoglossicida Infection

et al. 2021

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“…Sequencing Scaffold In addition to T6SS-related genes, researchers have found that genes encoding a member of the cold-shock protein family (cspA1; Qi et al 2019), sigma factor E (Huang et al 2019a), members of the zinc transport system ZnuCBA (znuA and znuC; He et al 2021;Huang et al 2021), an ATP-binding-cassette transporter family protein (pvdE; Huang et al 2018b), a sensor kinase belonging to a two-component regulatory system (gacS; Zhou et al 2023), and a transcriptional regulator-encoding gene (RK21_RS10325; Zhang et al 2019) are important virulence genes, wherein knockdown or knockout strains for the target gene revealed strongly attenuated virulence in Orange-spotted Groupers. Notably, artificial challenge with a dose of 1 × 10 5 colony-forming units/fish resulted in limited or no mortality of the fish.…”

Section: Strainmentioning

confidence: 99%

“…Various vaccines and antigens have been tested for protection against P. plecoglossicida infection (Table 3), including killed whole-cell (formalin-killed cell [FKC]) vaccines, major surface antigens of the pathogen (lipopolysaccharide, outer membrane proteins, and extracellular biofilm polysaccharide), and live attenuated vaccines (LAVs; Mao et al 2013aMao et al , 2013bHuang et al 2019a;Qi et al 2019;Ye et al 2021;Li et al 2022b;Wang et al 2022). Vaccination by the intraperitoneal (IP) injection route elicited significant antibody titers; however, all of the tested surface antigens and FKC bacterin failed to protect the vaccinated croakers, which exhibited a low relative percent survival (RPS) of 0-40% (Mao et al 2013a(Mao et al , 2013b.…”

Section: Pseudomonas Plecoglossicida Vaccine Trialsmentioning

confidence: 99%

“…Vaccination by the intraperitoneal (IP) injection route elicited significant antibody titers; however, all of the tested surface antigens and FKC bacterin failed to protect the vaccinated croakers, which exhibited a low relative percent survival (RPS) of 0-40% (Mao et al 2013a(Mao et al , 2013b. Efficient protection was only observed in treated groups that produced a significant cellular immune response; LAV candidates, such as ΔtssD, Δ4885ΔT6SS1, and rpoE/cspA1 RNAi strain, produced higher protection against experimental challenge; and the expression levels of immunity molecules CD8 and MHCI (major histocompatibility complex class I) were significantly upregulated, indicating better T-cell-mediated immunity (Huang et al 2019a;Qi et al 2019;Ye et al 2021;Li et al 2022a, T A B L E 3 Vaccine trials against Pseudomonas plecoglossicida infection. "(2)" indicates a double immunization treatment with a prime vaccination plus a boost vaccination.…”

Section: Pseudomonas Plecoglossicida Vaccine Trialsmentioning

confidence: 99%

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The bacterial pathogen Pseudomonas plecoglossicida, its epidemiology, virulence factors, vaccine development, and host–pathogen interactions

Mao,

Li,

et al. 2024

J Aqua Anim Hlth

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ObjectivePseudomoans plecoglossicida has been identified as a fish pathogen since 2000 and has caused serious infections in cultured Large Yellow Croakers Larimiththys crocea in coastal eastern China during recent years.MethodsPublished literatures of this pathogen have been reviewed.ResultSeveral strains with high genomic similarity have been isolated and identified; the bacteria induce natural infection at lower water temperatures (12.0–25.5°C) and induce numerous granulomas and nodules in the visceral organs of croakers. Researchers have investigated the epidemiology of P. plecoglossicida infection, identified major virulence factors, searched for pathogenic genes, analyzed host‐pathogen interactions, and endeavored to develop efficient vaccines.ConclusionThis paper provides an overview of these research advances to elucidate the virulence mechanisms of the pathogen and to promote vaccine development against infection.

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“…Pseudomonas plecoglossicida (formerly named Pseudomonas putida ) is a common aquatic pathogen that causes visceral granulomas disease in various fish such as Larimichthys crocea , Oncorhynchus mykiss , and Epinephelus coioides ( 26 – 28 ). Fish with visceral granulomas disease develop white nodules in the liver, kidneys, and spleen and causing high mortality ( 27 ).…”

Section: Introductionmentioning

confidence: 99%

Design combinations of evolved phage and antibiotic for antibacterial guided by analyzing the phage resistance of poorly antimicrobial phage

Xu,

Ding,

Shi

et al. 2023

Microbiol Spectr

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Although antibiotics are the primary method against bacterial infections, the rapid emergence of antibiotic resistance has forced interest in alternative antimicrobial strategies. Phage has been considered a new biological antimicrobial agent due to its high effectiveness in treating bacterial infections. However, the applications of phage therapy have been limited by the quick development of phage-resistant bacteria. Therefore, more effective phage treatment strategies need to be explored guided by characterizing phage-resistant mutants. In this study, Pseudomonas plecoglossicida phage vB_PpS_SYP was isolated from the sewage but exhibited weak antibacterial activity caused by phage-resistant bacteria. Phage-resistant mutants were isolated and their whole genomes were analyzed for differences. The results showed that mutations in glycosyltransferase family 1 ( GT-1 ) and hypothetical outer membrane protein ( homP ) led to bacterial phage resistance. The GT-1 mutants had lower biofilm biomass and higher antibiotic sensitivity than wild-type strain. Phage SYP evolved a broader host range and improved antimicrobial efficacy to infect homP mutants. Therefore, we designed a strategy for combined antibiotic and evolved phage inhibition driven by the two phage-resistant mutants. The results showed that the combination was more effective against bacteria than either antibiotics or phage alone. Our findings presented a novel approach to utilizing poorly antimicrobial phages by characterizing their phage-resistant mutants, with the potential to be expanded to include phage therapy for a variety of pathogens. IMPORTANCE The rapid emergence of antibiotic resistance renews interest in phage therapy. However, the lack of efficient phages against bacteria and the emergence of phage resistance impaired the efficiency of phage therapy. In this study, the isolated Pseudomonas plecoglossicida phage exhibited poor antibacterial capacity and was not available for phage therapy. Analysis of phage-resistant mutants guided the design of antibacterial strategies for the combination of antibiotics with evolved phages. The combination has a good antibacterial effect compared to the original phage. Our findings facilitate ideas for the development of antimicrobial-incapable phage, which have the potential to be applied to the phage treatment of other pathogens.

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Protection against Pseudomonas plecoglossicida in Epinephelus coioides immunized with a cspA1-knock-down live attenuated vaccine

Cited by 15 publications

References 19 publications

The Zinc Nutritional Immunity of Epinephelus coioides Contributes to the Importance of znuC During Pseudomonas plecoglossicida Infection

The Zinc Nutritional Immunity of Epinephelus coioides Contributes to the Importance of znuC During Pseudomonas plecoglossicida Infection

The bacterial pathogen Pseudomonas plecoglossicida, its epidemiology, virulence factors, vaccine development, and host–pathogen interactions

Design combinations of evolved phage and antibiotic for antibacterial guided by analyzing the phage resistance of poorly antimicrobial phage

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