Protection against L3 induced Brugia malayi infection in Mastomys coucha pre-immunized with BmAFII fraction of the filarial adult worm

Dixit, Saurabh; Gaur, Ritu; Sahoo, Malaya K.; Joseph, Sujith; Murthy, P. S. R.

doi:10.1016/j.vaccine.2006.05.003

Cited by 33 publications

(9 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Several vaccine candidates including the live parasites, crude extracts, semi-purified antigens as well as recombinant proteins have been tried in the past with partial success [30][31][32][33][34][35]. Multiple subunit vaccine candidates may permit immune targeting against multiple epitopes of specific immunogens together with more likelihood of stimulation of different layers of the immune responses at various levels.…”

Section: Discussionmentioning

confidence: 99%

Immunization with a multisubunit vaccine considerably reduces establishment of infective larvae in a rodent model of Brugia malayi

Shrivastava

Singh

Nag

et al. 2013

Comparative Immunology, Microbiology and Infectious Diseases

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

Immunization with a multisubunit vaccine considerably reduces establishment of infective larvae in a rodent model of Brugia malayi

Shrivastava

Singh

Nag

et al. 2013

Comparative Immunology, Microbiology and Infectious Diseases

View full text Add to dashboard Cite

“…The decreased L 3 -induced infection accompanied by the increase in specific IFN-c with a decrease in IL-10 release by cells of *62 kDa-immunized + L 3 -exposed animals as observed in the present study are indicative of the development of protective immunity. Our earlier study demonstrated that protection against L 3 imparted by BmAFII was via proinflammatory responses (Dixit et al 2006). On the other hand an elevated level of IgG and mixed cellular immune responses of the animals immunized with the other molecules (*22, *28, *32, and *45 kDa) + L 3 exposure support the parasitological findings.…”

Section: Discussionmentioning

confidence: 99%

“…The lymphocyte transformation test (LTT) using spleen cells was carried out to assess CMI broadly following the method of Klei et al (1990) with some modifications as described by Dixit et al (2006). The results were expressed as counts per minutes (cpm).…”

Section: Cell-mediated Immune Response (Cmi)mentioning

confidence: 99%

See 1 more Smart Citation

Identification of Brugia malayi adult worm molecules immunoreactive with sera of infected animals treated with antifilarial and re-infected with homologous infection and their role in the establishment of infection in Mastomys coucha

et al. 2007

Self Cite

View full text Add to dashboard Cite

The present study was aimed at identifying molecules of Brugia malayi adult worm (BmA) that are immunoreactive with sera of B. malayi-infected Mastomys coucha treated with albendazole (ALB) or diethylcarbamazine (DEC) and reexposed to infection and the effect of immunization with the molecules on the establishment of infection in M. coucha. A *62 kDa molecule showed strong reactivity with sera of infected ALB-treated reinfected animals whereas *32 kDa and *45 kDa molecules strongly reacted with sera of DEC-treated reinfected animals. Two molecules (*22 kDa and *28 kDa) reacted with sera of infected untreated and reinfected control animals. Immunization with *62 kDa molecules reduced the adult worm recovery by 58% (P \ 0.001) and microfilaremia by 32-54% (P \ 0.05-0.01) of unimmunized controls. Animals immunized with the *32 kDa molecule exhibited 63% recovery of adult worms with no effect on circulating microfilaraemia. L 3 inoculation in *62 kDa-immunized animals upregulated cellular proliferation, interferon-c (IFN-c) and IgG responses (P \ 0.001) but downregulated interleukin-10 (IL-10) response (P \ 0.001). Animals immunized with *22, *28, *32 and *45 kDa molecules and bearing L 3 -induced infection showed mixed responses. Lymph node cells of unimmunized animals exposed to *28, *32 and *62 kDa molecules showed significant DNA damage (P \ 0.001) as compared to untreated control; *62 kDa molecule induced maximum damage. In conclusion, immunization with a *62 kDa molecule suppressed the establishment of L 3 -induced infection in M. coucha and this correlated with enhanced cellular proliferation and IFN-c release, downregulation of IL-10, increased levels and DNA damage in lymph node cells.

show abstract

“…), and this was also associated with eosinophils and IL-5 [16]. In the experiments of Dixit el al [17], immunization of Mastomys coucha with a fraction of adult B. malayi extract reduced the recovery rate of adult worms by 85.7%. Other groups used recombinant peptides instead of complete extracts [18], [19], [20], [21].…”

Section: Introductionmentioning

confidence: 99%

Immunization with L. sigmodontis Microfilariae Reduces Peripheral Microfilaraemia after Challenge Infection by Inhibition of Filarial Embryogenesis

et al. 2012

View full text Add to dashboard Cite

BackgroundLymphatic filariasis and onchocerciasis are two chronic diseases mediated by parasitic filarial worms causing long term disability and massive socioeconomic problems. Filariae are transmitted by blood-feeding mosquitoes that take up the first stage larvae from an infected host and deliver it after maturation into infective stage to a new host. After closure of vector control programs, disease control relies mainly on mass drug administration with drugs that are primarily effective against first stage larvae and require many years of annual/biannual administration. Therefore, there is an urgent need for alternative treatment ways, i.e. other effective drugs or vaccines.Methodology/Principal FindingsUsing the Litomosoides sigmodontis murine model of filariasis we demonstrate that immunization with microfilariae together with the adjuvant alum prevents mice from developing high microfilaraemia after challenge infection. Immunization achieved 70% to 100% protection in the peripheral blood and in the pleural space and furthermore strongly reduced the microfilarial load in mice that remained microfilaraemic. Protection was associated with the impairment of intrauterine filarial embryogenesis and with local and systemic microfilarial-specific host IgG, as well as IFN-γ secretion by host cells from the site of infection. Furthermore immunization significantly reduced adult worm burden.Conclusions/SignificanceOur results present a tool to understand the immunological basis of vaccine induced protection in order to develop a microfilariae-based vaccine that reduces adult worm burden and prevents microfilaraemia, a powerful weapon to stop transmission of filariasis.

show abstract

Protection against L3 induced Brugia malayi infection in Mastomys coucha pre-immunized with BmAFII fraction of the filarial adult worm

Cited by 33 publications

References 42 publications

Immunization with a multisubunit vaccine considerably reduces establishment of infective larvae in a rodent model of Brugia malayi

Immunization with a multisubunit vaccine considerably reduces establishment of infective larvae in a rodent model of Brugia malayi

Identification of Brugia malayi adult worm molecules immunoreactive with sera of infected animals treated with antifilarial and re-infected with homologous infection and their role in the establishment of infection in Mastomys coucha

Immunization with L. sigmodontis Microfilariae Reduces Peripheral Microfilaraemia after Challenge Infection by Inhibition of Filarial Embryogenesis

Contact Info

Product

Resources

About