Protection against Homologous Influenza Challenge by Genetic Immunization with SFV‐RNA Encoding Flu‐HA

Immunogenicity of a DNA-launched replicon-based canine parvovirus DNA vaccine expressing VP2 antigen in dogs

“…Further, the lymphoproliferative response and CPV-specific priming of CD4 + /CD8 + effectors cells were significantly higher with repliconbased CPV DNA vaccine compared to conventional CPV DNA vaccine. Similar enhanced immune responses with replicon-based vaccines over conventional DNA vaccines have been reported earlier for other diseases (Zhou et al, 1994;Dalemans et al, 1995;Hariharan et al, 1998;Vignuzzi et al, 2001;Xiao et al, 2004;Arbele et al, 2005;Saxena et al, 2008).…”

Naked RNA immunization with replicons derived from poliovirus and Semliki Forest virus genomes for the generation of a cytotoxic T cell response against the influenza A virus nucleoprotein

“…Injection of naked RNA from the recombinant rSFV-NP replicon designed to express the influenza virus NP proved to be effective at inducing anti-NP antibodies after two injections; antibody titres were at levels comparable to those obtained by one injection of NP-encoding plasmid DNA. Induction of antibodies specific for an influenza virus protein by this type of vector when administered as naked RNA has already been described (Dalemans et al , 1995 ; Zhou et al , 1994 ). Recently, using LacZ as a model tumour antigen for cancer therapy, Ying et al (1999) have shown that injection of naked recombinant SFV RNA can elicit an antibody response, activate CD8 + T cells to release interferon-γ and protect from tumour challenge.…”

“…In this study, we evaluated the ability of recombinant replicons to induce both humoral and cellular immune responses when injected in the form of naked RNA, arguing that packaging these vectors is unnecessary, since their replicative nature alleviates the need for large quantities of input RNA. In the case of recombinant SFV vectors encoding the haemagglutinin and NP molecules of influenza A virus, the injection of naked RNA has been found to induce specific antibodies (Dalemans et al, 1995 ; Zhou et al, 1994 ). We showed here that two recombinant replicons, one derived from the SFV genome and the other from the poliovirus genome, which both encode the internal influenza A virus NP protein in place of the structural proteins, can elicit humoral responses after injection of naked RNA.…”

Infectious particles derived from Semliki Forest virus vectors encoding murine leukemia virus envelopes

“…One possible application for the kind of vector described here is in vaccination against retroviral Env. It has been shown that inoculation of animals with naked, SFV vector RNA can lead to an immune response to vector-encoded proteins (3,25). This process is presumably inefficient due to rapid degradation of naked vector RNA.…”