Protection against fowl cholera in ducks immunized with a combination vaccine containing live attenuated duck enteritis virus and recombinant outer membrane protein H of <i>Pasteurella multocida</i>

Apinda, Nisachon; Nambooppha, Boondarika; Rittipornlertrak, Amarin; Tankaew, Pallop; Punyapornwithaya, Veerasak; Nair, Venugopal; Sawada, Takuo; Sthitmatee, Nattawooti

doi:10.1080/03079457.2019.1711020

Cited by 11 publications

(12 citation statements)

References 24 publications

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“…Previous studies considering an ompH gene of P. multocida identified it as highly protective gene and suggested it to be a candidate vaccine for fowl cholera in duck which induced an efficient antibody response, able to reduce the degree of adhesion of the P. multocida strain to the duck embryo fibroblast cells, along with successfully inducing high levels of lymphocyte proliferation, providing clinical protection [ 10 , 11 ].…”

Section: Discussionmentioning

confidence: 99%

“…Available vaccines showed variable results in protecting ducks against fowl cholera in natural outbreaks [ 2 , 3 ]. The outer membrane protein H (OmpH), a major membrane protein of the P. multocida envelop [ 4 ] and a cross protective antigen among avian P. multocida strains [ 5 , 6 , 7 ], incorporated into flow cholera vaccine candidate, provided the strong protective immunity against P. multocida in chickens [ 5 , 6 , 8 , 9 ], ducks [ 10 , 11 ], and cattle [ 12 , 13 ]. Meanwhile, the live attenuated DEV vaccines are widely used for reduction of diseases impact in ducks [ 3 ].…”

Section: Introductionmentioning

confidence: 99%

“…The strategy the duck industry needs is to develop a combination of live attenuated vaccines to induce simultaneous protection against multiple diseases through the reduced administration of a multivalent vectored vaccine at reduced cost to improve poultry welfare. Previously, the effectiveness of mixed immunization against P. multocida and DEV was demonstrated [ 10 ]. However, new recombinant vaccines with the potential of inducing stronger humoral and cellular immune responses against these major pathogens are important.…”

Section: Introductionmentioning

confidence: 99%

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CRISPR/Cas9 Editing of Duck Enteritis Virus Genome for the Construction of a Recombinant Vaccine Vector Expressing ompH Gene of Pasteurella multocida in Two Novel Insertion Sites

et al. 2022

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Duck enteritis virus (DEV) and Pasteurella multocida, the causative agent of duck plague and fowl cholera, are acute contagious diseases and leading causes of morbidity and mortality in duck. The NHEJ-CRISPR/Cas9-mediated gene editing strategy, accompanied with the Cre–Lox system, have been employed in the present study to show that two new sites at UL55-LORF11 and UL44-44.5 loci in the genome of the attenuated Jansen strain of DEV can be used for the stable expression of the outer membrane protein H (ompH) gene of P. multocida that could be used as a bivalent vaccine candidate with the potential of protecting ducks simultaneously against major viral and bacterial pathogens. The two recombinant viruses, DEV-OmpH-V5-UL55-LORF11 and DEV-OmpH-V5-UL44-44.5, with the insertion of ompH-V5 gene at the UL55-LORF11 and UL44-44.5 loci respectively, showed similar growth kinetics and plaque size, compared to the wildtype virus, confirming that the insertion of the foreign gene into these did not have any detrimental effects on DEV. This is the first time the CRISPR/Cas9 system has been applied to insert a highly immunogenic gene from bacteria into the DEV genome rapidly and efficiently. This approach offers an efficient way to introduce other antigens into the DEV genome for multivalent vector.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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CRISPR/Cas9 Editing of Duck Enteritis Virus Genome for the Construction of a Recombinant Vaccine Vector Expressing ompH Gene of Pasteurella multocida in Two Novel Insertion Sites

et al. 2022

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“…Additionally, the histopathological lesions in the infected ducks were found to be more severe than those detected in the infected chickens which were characterized by multiple granulomata in most examined organs. The immunohistochemical (IHC) positive reaction against an antigen of P. multocida was more intensely stained and widely distributed in all examined organs of infected ducks than in chickens (Apinda et al 2020 ) and so on.…”

Section: Introductionmentioning

confidence: 99%

Pathognomonic features of Pasteurella multocida isolates among various avian species in Sharkia Governorate, Egypt

El-Demerdash,

Mowafy,

Fahmy

et al. 2023

World J Microbiol Biotechnol

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The present study aimed to isolate Pasteurella multocida (P. multocida) from pulmonary cases in several avian species and then investigate the histopathological features, antimicrobial resistance determinants, virulence characteristics, and risk factors analysis of the isolates in each species in correlation with epidemiological mapping of pasteurellosis in Sharkia Governorate, Egypt. The obtained data revealed a total occurrence of 9.4% (30/317) of P. multocida among the examined birds (chickens, ducks, quails, and turkeys). The incidence rate was influenced by avian species, climate, breed, age, clinical signs, and sample type. Antimicrobial susceptibility testing revealed that all isolates were sensitive to florfenicol and enrofloxacin, while 86.6 and 73.3% of the isolates displayed resistance to amoxicillin-clavulanic acid and erythromycin, respectively. All of the P. multocida isolates showed a multiple-drug resistant pattern with an average index of 0.43. Molecular characterization revealed that the oma87, sodA, and ptfA virulence genes were detected in the all examined P. multocida isolates. The ermX (erythromycin), blaROB-1 (β-lactam), and mcr-1(colistin) resistance genes were present in 60, 46.6, and 40% of the isolates, respectively. Ducks and quails were the most virulent and harbored species of antimicrobial-resistant genes. These results were in parallel with postmortem and histopathological examinations which detected more severe interstitial pneumonia lesions in the trachea and lung, congestion, and cellular infiltration especially in ducks. Epidemiological mapping revealed that the Fakous district was the most susceptible to pasteurellosis infection. Thus, farmers are recommended to monitor their flocks for signs of respiratory disease, seek veterinary care promptly if any birds are sick, and avoid the random usage of antibiotics. In conclusion, this study presents a comprehensive picture of the risk factors in correlation to the pathognomonic characteristics of P. multocida infection in poultry sectors to help in developing more effective strategies for prevention and control.

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“…Furthermore, OmpH stands out as a significant point of interest in the host’s immune defense, underscoring its importance in the context of pathogenesis and potential vaccine development. Numerous previous studies have shown that the use of recombinant OmpH (rOmpH) effectively stimulates robust protection in immunized chickens and ducks, resulting in a substantial induction of antibody-mediated protection [ 6 , 7 , 8 , 9 ]. Another major immunogenic antigen, OmpA, acts as a cross-protective antigen and serves as an outer membrane protein of P. multocida .…”

Section: Introductionmentioning

confidence: 99%

Efficiency of NHEJ-CRISPR/Cas9 and Cre-LoxP Engineered Recombinant Turkey Herpesvirus Expressing Pasteurella multocida OmpH Protein for Fowl Cholera Prevention in Ducks

Apinda,

Yao,

Zhang

et al. 2023

Vaccines

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Fowl cholera is caused by the bacterium Pasteurella multocida, a highly transmissible avian ailment with significant global implications, leading to substantial economic repercussions. The control of fowl cholera outbreaks primarily relies on vaccination using traditional vaccines that are still in use today despite their many limitations. In this research, we describe the development of a genetically engineered herpesvirus of turkeys (HVT) that carries the OmpH gene from P. multocida integrated into UL 45/46 intergenic region using CRISPR/Cas9-NHEJ and Cre-Lox system editing. The integration and expression of the foreign cassettes were confirmed using polymerase chain reaction (PCR), indirect immunofluorescence assays, and Western blot assays. The novel recombinant virus (rHVT-OmpH) demonstrated stable integration of the OmpH gene even after 15 consecutive in vitro passages, along with similar in vitro growth kinetics as the parent HVT virus. The protective efficacy of the rHVT-OmpH vaccine was evaluated in vaccinated ducks by examining the levels of P. multocida OmpH-specific antibodies in serum samples using ELISA. Groups of ducks that received the rHVT-OmpH vaccine or the rOmpH protein with Montanide™ (SEPPIC, Paris, France) adjuvant exhibited high levels of antibodies, in contrast to the negative control groups that received the parental HVT or PBS. The recombinant rHVT-OmpH vaccine also provided complete protection against exposure to virulent P. multocida X-73 seven days post-vaccination. This outcome not only demonstrates that the HVT vector possesses many characteristics of an ideal recombinant viral vaccine vector for protecting non-chicken hosts, such as ducks, but also represents significant research progress in identifying a modern, effective vaccine candidate for combatting ancient infectious diseases.

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Protection against fowl cholera in ducks immunized with a combination vaccine containing live attenuated duck enteritis virus and recombinant outer membrane protein H of Pasteurella multocida

Cited by 11 publications

References 24 publications

CRISPR/Cas9 Editing of Duck Enteritis Virus Genome for the Construction of a Recombinant Vaccine Vector Expressing ompH Gene of Pasteurella multocida in Two Novel Insertion Sites

CRISPR/Cas9 Editing of Duck Enteritis Virus Genome for the Construction of a Recombinant Vaccine Vector Expressing ompH Gene of Pasteurella multocida in Two Novel Insertion Sites

Pathognomonic features of Pasteurella multocida isolates among various avian species in Sharkia Governorate, Egypt

Efficiency of NHEJ-CRISPR/Cas9 and Cre-LoxP Engineered Recombinant Turkey Herpesvirus Expressing Pasteurella multocida OmpH Protein for Fowl Cholera Prevention in Ducks

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