Protection against Fasciola gigantica infection in mice by vaccination with recombinant juvenile-specific cathepsin L

Sansri, Veerawat; Meemon, Krai; Changklungmoa, Narin; Kueakhai, Pornanan; Chantree, Pathanin; Chaichanasak, Pannigan; Lorsuwannarat, Natcha; Itagaki, Tadashi; Sobhon, Prasert

doi:10.1016/j.vaccine.2015.02.010

Cited by 18 publications

(12 citation statements)

References 32 publications

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“…In mice, vaccinations with cathepsins B and L of F. hepatica and F. gigantica reduced the liver damage as indicated by decreased histopathology and the levels of liver enzymes including aspartate aminotransferase (AST), alanine transaminase (ALT), and gamma glutamyl transferase (GGT) (Jayaraj et al 2009;Sansri et al 2015). Similar results were also found in sheep vaccinated with adult-specific F. hepatica cathepsin Ls (Piacenza et al 1999).…”

Section: Vaccine Potentials Of Juvenile-specific Cathepsins B and Lsupporting

confidence: 53%

“…Similar results were also obtained for vaccination with recombinant rproFgCatL1H . The level of IgG in F. gigantica cathepsin vaccinations was found to be IgG1 dominant, indicating the Th2 response Sansri et al 2015). There was a strong negative correlation between the levels of total IgG and the numbers of worm recoveries .…”

Section: Vaccine Potentials Of Juvenile-specific Cathepsins B and Lmentioning

confidence: 90%

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Juvenile-specific cathepsin proteases in Fasciola spp.: their characteristics and vaccine efficacies

Meemon

Sobhon

2015

Parasitol Res

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Fasciolosis, caused by Fasciola hepatica and Fasciola gigantica, is one of the most neglected tropical zoonotic diseases. One sustainable control strategy against these infections is the employment of vaccines that target proteins essential for parasites' invasion and nutrition acquiring processes. Cathepsin proteases are the most abundantly expressed proteins in Fasciola spp. that have been tested successfully as vaccines against fasciolosis in experimental as well as large animals because of their important roles in digestion of nutrients, invasion, and migration. Specifically, juvenile-specific cathepsin proteases are the more effective vaccines because they could block the invasion and migration of juvenile parasites whose immune evasion mechanism has not yet been fully developed. Moreover, because of high sequence similarity and identity of cathepsins from juveniles with those of adults, the vaccines can attack both the juvenile and adult stages. In this article, the characteristics and vaccine potentials of juvenile-specific cathepsins, i.e., cathepsins L and B, of Fasciola spp. were reviewed.

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Section: Vaccine Potentials Of Juvenile-specific Cathepsins B and Lsupporting

confidence: 53%

Section: Vaccine Potentials Of Juvenile-specific Cathepsins B and Lmentioning

confidence: 90%

Juvenile-specific cathepsin proteases in Fasciola spp.: their characteristics and vaccine efficacies

Meemon

Sobhon

2015

Parasitol Res

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“…However, two other genes in MHC for the H-2 class I histocompatibility antigen and the H-2 class II histocompatibility antigen and three genes for cathepsin S-like, cathepsin K-like, and cathepsin L1 were down-regulated in both pathways of phagosomes and antigen processing and presentation. Cathepsins are proteases responsible for lysosome protein degradation and are widely distributed among prokaryotes and eukaryotes, in which cathespins play an immune function in fish and other vertebrates (Harikrishnan et al, 2010 ; Sansri et al, 2015 ; Wang et al, 2016 ). The decreased expression of cathepsin genes indicated a reduction in lysosome degradation.…”

Section: Discussionmentioning

confidence: 99%

Brain Transcriptome Profiling Analysis of Nile Tilapia (Oreochromis niloticus) Under Long-Term Hypersaline Stress

Liu

et al. 2018

Front. Physiol.

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The fish brain plays an important role in controlling growth, development, reproduction, and adaptation to environmental change. However, few studies stem from the perspective of whole transcriptome change in a fish brain and its response to long-term hypersaline stress. This study compares the differential transcriptomic responses of juvenile Nile tilapia (Oreochromis niloticus) maintained for 8 weeks in brackish water (16 practical salinity units, psu) and in freshwater. Fish brains from each treatment were collected for RNA-seq analysis to identify potential genes and pathways responding to hypersaline stress. A total of 27,089 genes were annotated, and 391 genes were expressed differently in the salinity treatment. Ten pathways containing 40 differentially expressed genes were identified in the tilapia brain. Antigen processing and presentation and phagosome were the two principally affected pathways in the immune system. Thirty-one of 40 genes were involved in various expressions associated with environmental information processing pathways such as neuroactive ligand-receptor interaction, cytokine-cytokine receptor interaction, the Jak-STAT signaling pathway, cell adhesion molecules (CAMs), and the PI3K-Akt signaling pathway, which are the upstream pathways for modulation of immunity and osmoregulation. The most-changed genes (>5-fold) were all down-regulated, including four growth hormone/prolactin gene families, i.e., prolactin precursor (−10.62), prolactin-1 (−11), somatotropin (−10.15), somatolactin-like (−6.18), and two other genes [thyrotropin subunit beta (−7.73) and gonadotropin subunit beta-2 (−5.06)] that stimulated prolactin release in tilapia. The downregulation pattern of these genes corroborates the decrease in tilapia immunity with increasing salinity and reveals an adaptive mechanism of tilapia to long-term hypersaline stress. Ovarian steroidogenesis, isoquinoline alkaloid biosynthesis, and phenylalanine metabolism are the three important pathways in the response of the fish to long-term hypersaline stress. This study has identified several pathways and relevant genes that are involved in salinity regulation in a euryhaline fish and provides insight into understanding regulatory mechanisms of fish to salinity change.

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“…Further, cathepsin L and tegument membrane proteins of the Opisthorchiid adult flukes are also similar, such tegument proteins were found protective against Fasciola hepatica in cattle and in mice, resulting in 48% and 67% worm burden reductions, respectively. 25,26 Similarly, a tegument protein "paramyosin" and a surface protein "enolase" of the Opisthorchiid parasite, C. sinensis, were used to immunize Sprague-Dawley rats, followed by infection challenge by the parasite, the vaccine resulted in 51% and 56% worm burden reductions, respectively. 27,28 In addition, recombinant cathepsin B cysteine F I G U R E 3 Uptake inhibition by neutralization of Opisthorchis viverrini extracellular vesicles proteinase-3 was also used as a protein subunit vaccine and resulted in 67% worm burden reduction.…”

Section: Protective Vaccines Related Parasites From the Opisthorchiid...mentioning

confidence: 99%

“…This suggests that protective antigens and/or vaccines against the other species could serve as a guide for protective antigen discovery against O. viverrini . Further, cathepsin L and tegument membrane proteins of the Opisthorchiid adult flukes are also similar, such tegument proteins were found protective against Fasciola hepatica in cattle and in mice, resulting in 48% and 67% worm burden reductions, respectively 25,26 . Similarly, a tegument protein “paramyosin” and a surface protein “enolase” of the Opisthorchiid parasite C. sinensis , were used to immunize Sprague–Dawley rats, followed by infection challenge by the parasite, the vaccine resulted in 51% and 56% worm burden reductions, respectively 27,28 .…”

Section: Vaccines and Infection Challenge Modelmentioning

confidence: 99%

Perspectives in the vaccine development against Opisthorchis viverrini liver fluke

Shalash

Skwarczynski

Tóth

2022

MedComm – Biomaterials and Applications

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Liver flukes, Opisthorchis viverrini, infect around 10 million individuals in Southeast Asia, alone, and cause 26,000 deaths in the region per year. Despite being classified as a Group 1 carcinogen, and presenting one of the leading causes of cholangiocarcinoma in epidemic areas, O. viverrini infection is a neglected tropical disease. Control measures were implemented in epidemic areas to limit the outspread of infection; however, prophylactic vaccines are urgently needed to protect against future reinfections. This holds especially true due to the limited curative efficacy of the approved anthelmintic drug. In this article, we have briefly summarized the recently reported information regarding hepatobiliary cancer pathogenesis, approved treatment, and control measures against infection. Further, we highlighted the progress in the identification of protective antigens against Opisthorchiasis and proposed the investigation of additional promising antigens relying on vaccine progress against related infectious parasites. We highlighted the relative efficacies of the developed preclinical vaccines, suggested alternative vaccine designs and combinations, and commented on the required immunological responses. Moreover, we also reviewed biomaterials used in vaccine delivery against O. viverrini infections, summarized all the reported vaccine design approaches against the disease, and provided future perspectives regarding vaccine development, the utilization of biomaterials, and the discovery of highly protective antigens.

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Protection against Fasciola gigantica infection in mice by vaccination with recombinant juvenile-specific cathepsin L

Cited by 18 publications

References 32 publications

Juvenile-specific cathepsin proteases in Fasciola spp.: their characteristics and vaccine efficacies

Juvenile-specific cathepsin proteases in Fasciola spp.: their characteristics and vaccine efficacies

Brain Transcriptome Profiling Analysis of Nile Tilapia (Oreochromis niloticus) Under Long-Term Hypersaline Stress

Perspectives in the vaccine development against Opisthorchis viverrini liver fluke

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