Protection afforded by a herbal medicine, Sho-seiryu-to (TJ-19), against oleic acid-induced acute lung injury in guinea-pigs

Chen, Yang; Ishitsuka, Yoichi; Moriuchi, Hiroshi; Golbidi, Saeid; Jin, Zhen-Ji; Irikura, Mitsuru; Irie, Tetsumi

doi:10.1211/jpp/61.07.0012

Cited by 6 publications

(8 citation statements)

References 35 publications

(49 reference statements)

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“…These include, but are not limited to, mice (Gong et al 2015;Letsiou et al 2015), rats (Xin et al 2015;Smith et al 2015), rabbits (Walther et al 2014;Uzawa et al 2015), guinea pigs (Kabir et al 2009;C. Q. Yang et al 2009), dogs (Y.…”

Section: Principal Features Used To Define Ards In Animal Modelsmentioning

confidence: 99%

Mouse Models of Acute Respiratory Distress Syndrome

Aeffner¹,

2015

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Acute respiratory distress syndrome (ARDS) is a severe pulmonary reaction requiring hospitalization, which is incited by many causes, including bacterial and viral pneumonia as well as near drowning, aspiration of gastric contents, pancreatitis, intravenous drug use, and abdominal trauma. In humans, ARDS is very well defined by a list of clinical parameters. However, until recently no consensus was available regarding the criteria of ARDS that should be evident in an experimental animal model. This lack was rectified by a 2011 workshop report by the American Thoracic Society, which defined the main features proposed to delineate the presence of ARDS in laboratory animals. These should include histological changes in parenchymal tissue, altered integrity of the alveolar capillary barrier, inflammation, and abnormal pulmonary function. Murine ARDS models typically are defined by such features as pulmonary edema and leukocyte infiltration in cytological preparations of bronchoalveolar lavage fluid and/or lung sections. Common pathophysiological indicators of ARDS in mice include impaired pulmonary gas exchange and histological evidence of inflammatory infiltrates into the lung. Thus, morphological endpoints remain a vital component of data sets assembled from animal ARDS models.

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Section: Principal Features Used To Define Ards In Animal Modelsmentioning

confidence: 99%

Mouse Models of Acute Respiratory Distress Syndrome

Aeffner¹,

2015

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“…12,13) From this evidence grew the hypothesis that the protection afforded by PEP in animal models of these disorders was due to it counteracting the effects of ROS. This study was conducted to examine whether PEP was able to exert anti-oxidative activity and attenuate cell injury induced by oxidative stress.…”

Section: )mentioning

confidence: 98%

“…[7][8][9][10] Oxidative stress seems to be a potent factor in the development of organ ischemia/reperfusion injuries 11) and of acute lung injury induced by oleic acid. 12,13) From this evidence grew the hypothesis that the protection afforded by PEP in animal models of these disorders was due to it counteracting the effects of ROS. This study was conducted to examine whether PEP was able to exert anti-oxidative activity and attenuate cell injury induced by oxidative stress.…”

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confidence: 98%

Phosphoenolpyruvic Acid, an Intermediary Metabolite of Glycolysis, as a Potential Cytoprotectant and Anti-oxidant in HeLa Cells

Kondo

Ishitsuka

Kadowaki

et al. 2012

Biological & Pharmaceutical Bulletin

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This study examined the cytoprotective and anti-oxidative properties of phosphoenolpyruvic acid (PEP), a glycolysis metabolite with a high-energy phosphate group. PEP (0.1-10 mM) significantly attenuated the decrease in cell viability induced by hydrogen peroxide (H 2 O 2 ) in HeLa cells in a dose-dependent manner. PEP also inhibited the decrease in calcein-acetomethoxy-stained cells and the increase in propidium iodidestained cells that were induced by H 2 O 2 . The H 2 O 2 -stimulated increase in intracellular reactive oxygen species was significantly reduced by PEP. PEP also demonstrated scavenging potential against hydroxyl radicals, as assessed by the electron paramagnetic resonance method. In addition, PEP demonstrated scavenging potential against the 1,1-diphenyl-2-picrylhydrazyl radical, a representative artificial radical, although the potential is very weak. PEP (10 mM) slightly inhibited the decrease in cellular ATP content induced by H 2 O 2 , but did not show any effects at low doses (0.1, 1 mM). PEP (0.1-10 mM) also attenuated the cell injury but not the decrease in intracellular ATP content, induced by 2-deoxy-D-glucose, a glycolysis inhibitor. These results indicate that PEP exerts cytoprotective effects and has anti-oxidative potential, although the precise cytoprotective mechanisms are not fully elucidated. We suggest that PEP is a functional carbohydrate metabolite with cytoprotective and anti-oxidative activity, and is potentially useful as a therapeutic agent against diseases that involve the oxidative stress.Key words phosphoenolpyruvic acid; anti-oxidant; oxidative stress; cellular injury; hydrogen peroxide Phosphoenolpyruvic acid (PEP) is a high-energy intermediate substance in the glycolytic and gluconeogenic pathways. PEP can penetrate the cell membrane and transfer its highenergy phosphate group to adenosine diphosphate, aiding the replenishment of intracellular ATP.1,2) It has been reported that PEP improved post-ischemic energy status in the heart, 3) skeletal muscle, 4) and liver 1) in rats. Hojo et al. 5) demonstrated that transplanted kidney function and graft survival were improved by PEP in experimental kidney transplantation in a canine model. In addition, we demonstrated that PEP attenuated pulmonary gas exchange dysfunction and pulmonary vascular hyperpermeability in acute lung injury induced by oleic acid in the guinea pig, a model of acute lung injury. 6)These findings indicate that PEP is an attractive prospect as a putative agent that protects cells and organs from damage by improving their energy status. However, the mechanism(s) of its protective effects have not been well defined.Reactive oxygen species (ROS) such as hydrogen peroxide (H 2 O 2 ) and the hydroxyl radical (·OH) are highly reactive substances that play important roles in some physiological conditions. However, they also contribute to pathophysiological damage as inducers of oxidative stress.7-10) Oxidative stress seems to be a potent factor in the development of organ ischemia/reperfusion injuries 11) a...

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“…9,12) Briefly, a granule of TJ-19 (1.0 g) was extracted with methanol (20 ml) under ultrasonication for 30 min and then centrifuged at 3000 rpm for 5 min. The supernatant was filtrated with a membrane filter (0.45 mm) and then submitted for HPLC analysis (30 ml).…”

Section: Three-dimensional Hplc Analysismentioning

confidence: 99%

“…[9][10][11] Our recent study indicated that TJ-19 drastically attenuated oleic acidinduced lung injury, a representative animal model of acute lung injury with severe hypoxemia and pulmonary edema, at least in part through antioxidative and anti-inflammatory effects. 12) Based on these lines of evidence, TJ-19 may also be effective against inflammatory pulmonary diseases; however, little has been reported on its effects against pulmonary fibrosis.…”

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confidence: 99%