Protectins: Past, Present Problems, and Perspectives*

Prokop, O; Uhlenbruck, G; Rothe, Anselm; Cohen, E. G. D.

doi:10.1111/j.1749-6632.1974.tb53035.x

Cited by 11 publications

(7 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In medaka eggs, Shibata et al (2000) reported the existence of a specific metalloproteinase, the alveolin, also responsible for chorion hardening following fertilisation. The fact that alveoli lectins and glycoconjugates are extruded into the perivitelline space at fertilisation could indicate that they are not an important source of nutrients to the embryos (Yamamoto, 1962), but different glycoconjugates may be involved in sperm and bacteria agglutination (Prokop et al, 1974) or in binding of sperm to the egg surface, as suggested by Kothbauer and Schenkel-Bruner (1974).…”

Section: Discussionmentioning

confidence: 97%

“…The presence of high molecular mass polysialoglycoproteins or H-hyosophorins has been reported in Fundulus heteroclitus , in medaka, Oryzias latipes and in several salmonids (Inoue et al, 1987;Kitajima et al, 1988). Glycoconjugates may be involved in binding of hormones; in bacteria agglutination; in the transport of metabolites and ions across the plasmalema; in sperm-egg binding; and in polyspermic inhibition (Prokop et al, 1974;Miller and Ax, 1990;Skutelsky et al, 1994;Motta et al, 2005).…”

Section: Introductionmentioning

confidence: 98%

See 1 more Smart Citation

Caracterización histoquímica de ovocitos del pez espada, <i>Xiphias gladius</i>

Ortiz-Delgado¹,

Porcelloni²,

Fossi³

et al. 2008

Sci. Mar.

View full text Add to dashboard Cite

SUMMARY: This paper reports a histological, histochemical and immunohistochemical characterisation of growing oocytes of the swordfish Xiphias gladius. The presence and distribution of carbohydrates, proteins, lipids, calcium, iron, vitellogenin/ Vg, zona radiata protein/Zrp, metallothionein/Mt, and thyroid hormones/T 3 -T 4 were studied during oogenesis (cortical alveoli, globules, yolk-granules, cytoplasm, follicular and radiata envelopes). During the initial vitellogenic phase, the oocytes showed cortical alveoli and oil globules containing neutral lipids exclusively. During this phase, small yolk granules appeared around the peripheral cytoplasm, and they increased through exogenous vitellogenesis. Yolk granules were composed of glycoproteins, calcium, iron, and proteins rich in lysine, arginine, tyrosine, tryptophan, cysteine and cystine. Vg and Mt were immunohistochemically detected in yolk. The follicular envelope contained proteins rich in amino acids. Moreover, calcium and thyroid hormones (triiodotyronine and thyroxine/T 3 , T 4 ) were detected in this cell envelope. Cortical alveoli, which contained carboxylated and neutral glycoconjugates, were especially rich in N-acetyl-D-galactosamine, N-acetyl-Dglucosamine, galactose and sialic acid. Finally, the zona radiata was mainly proteinaceous in nature and was composed of calcium and neutral glycoproteins. The egg envelope or chorion and the liver showed specific immunoreactivities by using anti-salmon Zrp as the primary antiserum.Keywords: histochemistry, carbohydrates, lipids, glycoconjugates, vitellogenin, zona radiata protein, methallothionein, thyroid homones, cations, oocytes, Xiphias gladius. RESUMEN: Caracterización histoquímica de ovocitos del pez espada, Xiphias gladius. -En este trabajo se realiza una caracterización histológica, histoquímica e inmunohistoquímica de los ovocitos en crecimiento del pez espada, Xiphias gladius. Se estudia la presencia y distribución de carbohidratos, proteínas, lípidos, calcio, hierro, vitelogenina/Vg, proteína radiada/Zrp, metalotioneina/Mt y hormonas tiroideas/ T 3 -T 4 durante la ovogénesis (alveolos corticales, glóbulos, gránulos-vitelo, citoplasma, capas folicular y radiada). Durante la fase inicial de la vitelogénesis, los ovocitos presentan alveolos corticales y una gota lipídica conteniendo exclusivamente lípidos neutros. Durante esta fase en la periferia del citoplasma aparecen pequeños granulos de vitelo, incrementando progresivamente durante la vitelogénesis exógena. Los gránulos de vitelo están compuestos por glicoproteínas, calcio, hierro, proteínas ricas en lisina, arginina, tirosina, triptófano, cisteina y cistina, y contienen Vg y Mt. La capa folicular de los ovocitos del pez espada está compuesta por glicoproteínas y proteínas ricas en diferentes aminoácidos. Esta envuelta contiene calcio y hormonas tiroideas/ T 3 y T 4 . Los alveolos corticales están constituidos por glicoconjugados carboxilados y neutros, y son especialmente ricos en N-acetil-D-galactosamina, N-acetil-D-glucosamina, galactosa y...

show abstract

Section: Discussionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 98%

Caracterización histoquímica de ovocitos del pez espada, <i>Xiphias gladius</i>

Ortiz-Delgado¹,

Porcelloni²,

Fossi³

et al. 2008

Sci. Mar.

View full text Add to dashboard Cite

show abstract

“…The serological specificities of the egg material were determined by human blood group specific antisera as well as agglutinins of animal and plant origin. Agglutinins from plant seeds (lectins) and agglutinins associated with animal reproductive organs and their products (protectins, see Prokop et al, 1968Prokop et al, , 1974 in many cases show a relatively high blood group specificity when reacting with native human erythrocytes. But, nevertheless, a wider range of reactivity is often observable (for example the anti-A from the albumen gland of Helix pomatia is specific for human blood group A-erythrocytes (Prokop et al, 1965) reacting thereby with a-N-acetylgalactosamine as the determinant group, but can be purified by adsorption on Dextran (Sephadex) gels (Ishiyama & Uhlenbruck, 1972; see also Prokop et al, 1974)).…”

Section: Results a N D Discussionmentioning

confidence: 99%

IMMUNOCHEMICAL INVESTIGATIONS ON TOAD (BUFO) EGGS: COMPARATIVE STUDIES ON THREE SPECIES (B. BUFO, B. VIRIDIS, B. CALAMITA)

Schenkel‐Brunner

Kothbauer

1976

Int J Immunogenetics

View full text Add to dashboard Cite

show abstract

“…Human blood group specificity, which is marked in albumin gland hemagglutinins from certain gastropod mollusks (Prokop, Uhlenbruck, Rothe and Cohen, 1974), is lacking in H. pyriformis. This lack of specificity has been reported in other urochordates (Wright, 1974) and is typical of most hemolymph lectins (Brown, Almodovar, Bhatia and Boyd, 1968;Tripp, 1966;Hall and Rowlands, 1974b).…”

Section: Discussionmentioning

confidence: 99%