Protecting spermatogenesis from damage induced by doxorubicin using the luteinizing hormone-releasing hormone agonist leuprorelin

Manabe, Fumio; Takeshima, Hitoshi; Akaza, Hideyuki

doi:10.1002/(sici)1097-0142(19970301)79:5<1014::aid-cncr19>3.0.co;2-4

Cited by 24 publications

(10 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[318–21] The major pathogenic mechanism for its toxicity appears to involve the generation of reactive oxygen species. A number of natural and synthetic compounds are known to alleviate Dox-induced toxicity,[22–26] of which polyphenols possessing significant free radical scavenging activity are considered important. [27] A number of animal models including mice, rats, dogs, swine, hamsters, and rabbits have been used for studying Dox-induced organotoxicity.…”

Section: Resultsmentioning

confidence: 99%

Protective effects of Ficus racemosa stem bark against doxorubucin-induced renal and testicular toxicity

2013

View full text Add to dashboard Cite

Background:Ficus racemosa Linn. (Moraceae) bark is a rich source of phenolic compounds known to possess potential antioxidant activity offering numerous health benefits.Materials and Methods:The present study evaluated the protective effects of sequential acetone extract of Ficus racemosa bark at two doses (FR250; 250 mg kg-1 and FR500; 500 mg kg-1 p.o.) against doxorubicin-induced renal and testicular toxicity in rats.Results:Doxorubicin administration resulted in significant decrease (P ≤ 0.05) in total protein and glutathione concentrations, while increased (P ≤ 0.05) serum urea, creatinine and thiobarbituric acid reactive substances (TBARS). Extract pretreatment restored biochemical parameters toward normalization. FR250 and FR500 decreased serum creatinine levels by 22.5% and 44%, while serum urea levels were decreased by 30.4% and 58.8%, respectively. Extract pretreatment (500 mg kg-1) decreased TBARS and increased glutathione levels in the kidney and testis to control levels. These observations were substantiated by histopathological studies, wherein normal renal and testicular architecture was restored in FR500 group.Conclusion:Doxorubicin exposure results in pronounced oxidative stress, and administration of F. racemosa stem bark extract offers significant renal and testicular protection by inhibiting lipidperoxidation-mediated through scavenging free radicals.

show abstract

Section: Resultsmentioning

confidence: 99%

Protective effects of Ficus racemosa stem bark against doxorubucin-induced renal and testicular toxicity

2013

View full text Add to dashboard Cite

show abstract

“…Busulfan is an anticancer drug that is used for several malignant diseases, especially for chronic myelogenous leukemia and polycythemia vera 22 . In many animal experiments, other drugs such as cyclophosphamide, doxorubicin, cisplatin, or procarbazine, have occasionally been used to damage spermatogenesis 2–7,23 . We have chosen busulfan in the present study, because it damages early stage germ cells more severely than other drugs 17 .…”

Section: Discussionmentioning

confidence: 99%

GnRH analog, leuprorelin acetate, promotes regeneration of rat spermatogenesis after severe chemical damage

et al. 2001

View full text Add to dashboard Cite

Background: Future fertility is a major concern for cancer patients who undergo intensive chemotherapy. There has been controversy about whether hormonal treatments may have protective effects against the severe spermatogenic damage caused by chemotherapy or irradiation. Recently, it has been proposed that gonadotrophin-releasing hormone (GnRH) analogs administered after testicular damage stimulate the recovery of spermatogenesis. In this study, we have investigated the effects of GnRH agonist, leuprorelin, on the damage to spermatogenesis induced by busulfan. Methods: Fisher rats were treated with busulfan, 25 mg/kg, intraperitoneally. The effects of subcutaneous injections of leuprorelin before or after treatment were evaluated histologically 18 weeks later. Results:The percentage of 'recovered' seminiferous tubules was 27.7 ± 12.6% in control rats without leuprorelin and 26.9 ± 10.2% in rats with leuprorelin injected 4 weeks before busulfan. Rats in both groups showed poor recovery of spermatogenesis with an increase of intratesticular fluid. However, rats treated with leuprorelin three times (4 weeks apart) after busulfan showed an improvement of up to 56.5 ± 12.0% (P < 0.05). A focal but massive necrotic lesion in the testis was observed only in this group of rats. Conclusions:The results demonstrated that leuprorelin administered after chemical testicular damage enhanced the recovery of spermatogenesis. At the same time, a possible significant side-effect of leuprorelin was noted.

show abstract

“…Following graded dehydration through alcohol, the testicular tissues were embedded in paraffin and the blocks were retained until they were cut into sections for histochemical staining with hematoxylin and eosin (HE). The sections were evaluated by light microscopy with particular attention to the maturity of germ cells in seminiferous tubules using Johnsen's score (Johnsen, 1970;Manabe et al, 1997). The Johnsen's score is a subjective histopathology score of the testicular tissue revealing the maturation of the seminiferous tubules ranged from 0 to 10 (Table 1).…”

Section: Methodsmentioning

confidence: 99%

The effect of quince leaf (<i>Cydonia oblonga</i> miller) decoction on testes in hypercholesterolemic rabbits: A pilot study

Ashrafi¹,

Ghabili²,

Alihemmati³

et al. 2013

Afr. J. Trad. Compl. Alt. Med.

View full text Add to dashboard Cite

Current medical literature lacks any evidence of the protective effects of quince leaf on testes. Therefore, the aim of the present study was to assess the effect of quince (Cydonia oblonga Miller) leaf decoction on testicular injury and impaired spermatogenesis induced by hypercholesterolemia in rabbits. Eleven mature New Zealand white male rabbits were randomly divided into three groups: group 1 (hypercholesterolemia, n=3), group 2 (hypercholesterolemia plus quince treatment, n=6), and group 3 (control, n=2). Groups 1 and 2 received a cholesterol-enriched diet for six weeks. Group 2 received C. oblonga leaf decoction as drinking supplement as well. After six weeks, a normal diet was substituted in groups 1 and 2 for another six weeks. Group 3 (control group) was maintained throughout the study on a regular diet. At the end of the 12 th week, the left testes of the animals were resected for light microscopic study with particular attention to the maturity of germ cells in seminiferous tubules using Johnsen's score. Increase in intertubular connective tissue and diameter of vessels, abundant spermatogonia and primary spermatocytes along the reduced germinal epithelium were noted in all rabbits of the group 1. The remaining animals in groups 2 and 3 had no significant changes in their testicular sections. The mean Johnsen's score of group 1 (4.20±1.92) was significantly lower than that of group 2 (7.33±0.52) and group 3 (7.05±0.07). (P=0.01). In conclusion, quince leaf decoction (C. oblonga Miller) protected rabbit testes and spermatogenesis from damage induced by hypercholesterolemia.

show abstract

Protecting spermatogenesis from damage induced by doxorubicin using the luteinizing hormone-releasing hormone agonist leuprorelin

Cited by 24 publications

References 20 publications

Protective effects of Ficus racemosa stem bark against doxorubucin-induced renal and testicular toxicity

Protective effects of Ficus racemosa stem bark against doxorubucin-induced renal and testicular toxicity

GnRH analog, leuprorelin acetate, promotes regeneration of rat spermatogenesis after severe chemical damage

The effect of quince leaf (<i>Cydonia oblonga</i> miller) decoction on testes in hypercholesterolemic rabbits: A pilot study

Contact Info

Product

Resources

About