Protecting potato plants against PVX and PVY viral infections by the application of native and chemically modified legume proteins

Sitohy, Mahmoud; Taha, S.; Abdelhamid, Mahmoud; Abdelbacki, Ashraf M. M.; Hamed, A. H.; Osman, Ali

doi:10.1007/s41348-021-00448-9

Cited by 5 publications

(5 citation statements)

References 58 publications

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“…The relative amounts of polar acidic, basic, and hydrophobic amino acids in FNP represent about 31.6, 14.3, and 28.5% against 33, 26.5, and 36.33% in MNP, respectively [55,56]. The antibacterial activity of the native protein may be due to its content of positively charged cationic and hydrophobic residues of amino acids [57][58][59][60]. The MICs of FNP and MNP were recorded between 5000 and 10,000 µg/mL.…”

Section: Discussionmentioning

confidence: 99%

Silver-Protein Nanocomposites as Antimicrobial Agents

Sitohy

Al-Mohammadi²,

Osman

et al. 2021

Nanomaterials

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The use of nanomaterials alone or in composites with proteins is a promising alternative to inhibit pathogenic bacteria. In this regard, this study used seed proteins from both fenugreek (Trigonella foenum-graecum L.) (FNP) and mung bean (Viga radiate) (MNP), with silver nanoparticles (Ag-NPs) and nanocomposites of either Ag-NPs plus FNP (Ag-FNP) or Ag-NPs plus MNP (Ag-MNP) as inhibitory agents against pathogenic bacteria. FNP and MNP were isolated from fenugreek seeds and mung bean seeds, respectively, and fractionated using Sodium Dodecyl Sulfate–Polyacrylamide Gel Electrophoresis (SDS–PAGE). Both FNP and MNP were immobilized with Ag-NPs to synthesize the nanocomposites Ag-FNP and Ag-MNP, respectively. The physicochemical characteristics of Ag-NPs and their composites with proteins were studied by X-ray Diffraction (XRD), dynamic light scattering (DLS), the zeta potential, Scanning and Transmission Electron Microscopy (SEM and TEM, respectively), Atomic Force Microscopy (AFM), and the Brunauer–Emmett–Teller isotherm (BET), elucidating their structural parameters, size distribution, size charges, size surface morphology, particle shape, dimensional forms of particles, and specific surface area, respectively. The sole proteins, Ag-NPs, and their nanocomposites inhibited pathogenic Gram-positive and Gram-negative bacteria. The inhibitory activities of both nanocomposites (Ag-FNP and Ag-MNP) were more than those obtained by either Ag-NPs or proteins (FNP, MNP). Minimum inhibitory concentrations (MICs) of Ag-FNP were very low (20 and 10 µg mL−1) against Salmonellatyphimurium and Pseudomonasaerugenosa, respectively, but higher (162 µg mL−1) against E. coli and Listeriamonocytogenes. MICs of Ag-MNP were also very low (20 µg mL−1) against Staphylococcusaureus but higher (325 µg mL−1) against Listeriamonocytogenes. TEM images of Staphylococcusaureus and Salmonellatyphimurium, treated with Ag-FNP and Ag-MNP, at their MIC values, showed asymmetric, wrinkled exterior surfaces, cell deformations, cell depressions, and diminished cell numbers.

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Section: Discussionmentioning

confidence: 99%

Silver-Protein Nanocomposites as Antimicrobial Agents

Sitohy

Al-Mohammadi²,

Osman

et al. 2021

Nanomaterials

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“…The value of the isoelectric point (IEP) of lupine 11S is higher than other previously studied similar fractions isolated from legumes, e.g., soy protein (Osman et al, 2016 ) and chickpea protein (Sitohy et al, 2011 ), explaining the difference in the antibacterial activity according to legume sources. Consequently, the IEP of the methylated (M11S) excelled over the corresponding methylated fractions from other legume sources (Sitohy et al, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

“…The four investigated gram-negative bacteria (Klebsiella oxytoca, Salmonella typhimurium, Pseudomonas aeruginosa, and Proteus mirabilis) were specifically susceptible to gentamycin, ciprofloxacin, ciprofloxacin, and ciprofloxacin at MIC , , , and µg/ml, respectively. methylated fractions from other legume sources (Sitohy et al, 2021).…”

Section: Figurementioning

confidence: 99%

Controlling bacterial biofilm formation by native and methylated lupine 11S globulins

Enan,

Abdel-Shafi,

El-Nemr

et al. 2023

Front. Microbiol.

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The antibacterial and anti-biofilm activities of the 11S globulins isolated from lupin seeds (Lupinus termis), and its methylated derivative (M11S), were investigated against seven pathogenic gram-positive and gram-negative bacteria. The MIC of 11S ranged from 0.1 to 4.0 μg/ml against 0.025 to 0.50 μg/ml for M11S, excelling some specific antibiotics. The MICs of M11S were 40–80 times lower than some specific antibiotics against gram-positive bacteria and 2–60 times lower than some specific antibiotics against gram-negative bacteria. One MIC of 11S and M11S highly reduced the liquid growth of all tested bacteria during 24 h at 37°C. They also inhibited biofilm formation by 80%−86% and 85%−94%, respectively (gram-positive), and 29%−44% and 43%−50%, respectively (gram-negative). M11S prevented biofilm formation by gram-positive bacteria at minimum biofilm inhibitory concentration (MBIC), 0.025–0.1 μg/ml against 0.1–0.5 μg/ml for gram-negative bacteria, i.e., 4–20 times and 4–7 times anti-biofilm inhibitory action compared with 11S, respectively. Biofilm formation of two bacteria revealed no adhered cells on glass slides for 24 h at 37°C, i.e., was entirely prevented by one MBIC of 11S and M11S. Scanning electron microscopy indicated microbial biofilm deformation under the action of 11S and M11S, indicating their broad specificity and cell membrane-targeted action.

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“…Due to its amino groups' protonation, chitosan is soluble in dilute solutions of some organic acids at (pH 6), and it can interact with polyanions to form polyelectrolyte complexes because of its polycationic nature ( Peniche and Argüelles-Monal, 2001 ). The cationic character of chitosan might impart it with valuable antiviral or antibacterial activities in a manner similar to cationic proteins, previously reported having antibacterial ( Sitohy et al, 2011 , Sitohy et al, 2012 , Sitohy et al, 2013 ; Abdel-Shafi et al, 2016 , Osman et al, 2016 ) and antiviral activities ( Abdelbacki et al, 2010 , Chobert et al, 2007 ; Swelum et al, 2020 ; Taha et al, 2010 , Sitohy et al, 2006 , Sitohy et al, 2007 , Sitohy et al, 2020 , Sitohy et al, 2021a , Sitohy et al, 2021b ).…”

Section: Introductionmentioning

confidence: 97%