Protecting effect of vitamin E supplementation on submaximal exercise-induced oxidative stress in sedentary dogs as assessed by erythrocyte membrane fluidity and paraoxonase-1 activity

Motta, Silvia; Letellier, Catherine; Ropert, Martine; Motta, Claude; Thiébault, J.J.

doi:10.1016/j.tvjl.2008.03.013

Cited by 24 publications

(18 citation statements)

References 43 publications

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“…Membrane fluidity of RBC obtained from competition horses decreased immediately after a standardized exercise, which included a final step at maximal velocity performed on a race track (De Moffarts et al 2007). Similar observations, rigidity in erythrocyte membranes, were found in four sedentary dogs after submaximal exercise in a study attempting to elucidate the role of vitamin E supplementation in preventing the effects of submaximal exercise-induced oxidative damage over a long period of time (Motta et al 2009). Finally, Brzeszczynska et al (2008) detected erythrocyte rigidity 1 h after an exhaustive incremental cycling test performed in 11 healthy untrained males.…”

Section: Discussionmentioning

confidence: 59%

“…Finally, acute exercise has been proved to elevate MDA concentrations or several reactive products of lipid peroxidation in the active muscle (Alessio et al 2000;Davies et al 1982;Ji and Fu 1992;Li et al 1999) as well as other tissues and plasma (Brzeszczynska et al 2008;Motta et al 2009). In contrast to these observations, but in accordance with our results, other researchers have found that exercise minimally modifies or even reduces the degree of lipid peroxidation, presumably by increasing the capacity of the antioxidant system (Bloomer et al 2007;Groussard et al 2003;Kim et al 1996) or due to increased clearance or distribution throughout the body (Leaf et al 1997).…”

Section: Discussionmentioning

confidence: 99%

“…Thus, cell membranes isolated from RBC may reflect, at least partially, processes ongoing at various sites in the organism such as skeletal muscles. For this reason and because of its ready availability, membranes isolated from RBC have been used to perform experimental studies on the membrane function in animals (Motta et al 2009;Portier et al 2006) and in humans (Brzeszczynska et al 2008). None of these studies have compared different types of exercise and correlated the membrane function with the plasma oxidative damage.…”

Section: Introductionmentioning

confidence: 99%

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Erythrocyte membrane fluidity and indices of plasmatic oxidative damage after acute physical exercise in humans

et al. 2010

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Optimal levels of membrane fluidity are essential for numerous cell functions including cell growth, solute transport and signal transduction. Since exercise enhances free radical production, our aim was to evaluate in healthy male subjects the effects of an acute bout of maximal and submaximal exercise on the erythrocyte membrane fluidity and its possible relation to the oxidative damage overproduction due to exercise. Subjects (n = 34) performed three cycloergometric tests: a continuous progressive exercise, a strenuous exercise until exhaustion and an acute bout of exercise at an intensity corresponding to 70% of maximal work capacity for 30 min. Venous blood samples were collected before and immediately after these exercises. Erythrocyte membrane fluidity was assessed by fluorescence spectroscopy. Plasma malondialdehyde (MDA) and 4-hydroxyalkenals (4-HDA) concentrations and carbonyl content of plasmatic proteins were used as an index of lipid and protein oxidation, respectively. Exercise produced a dramatic drop in the erythrocyte membrane fluidity as compared to resting time, but this was not accompanied by significant changes in the plasmatic MDA and 4-HDA concentrations. The highest erythrocyte membrane rigidity was detected immediately after strenuous exercise until exhaustion was performed. Protein carbonyl levels were higher after exhaustive exercises than at rest. Continuous progressive and strenuous exercises until exhaustion, but not submaximal workload, resulted in a significant enhanced accumulation of carbonylated proteins in the plasma. These findings are consistent with the idea that exercise exaggerates oxidative damage, which may contribute, at least partially, to explain the rigidity in the membrane of the erythrocytes due to acute exercise.

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Section: Discussionmentioning

confidence: 59%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Erythrocyte membrane fluidity and indices of plasmatic oxidative damage after acute physical exercise in humans

et al. 2010

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“…This vitamin also recovered the decrease in PON1 induced by exercise in untrained dogs [71]. Likewise, oral supplementation of vitamin E (200 mg/day) prevented the exercise-induced decrease in PON1/Aryl esterase activity in basketball players [72].…”

Section: Vitaminsmentioning

confidence: 99%

The Search for Dietary Supplements to Elevate or Activate Circulating Paraoxonases

Lou-Bonafonte

Gabás‐Rivera

Navarro

et al. 2017

IJMS

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Low levels of paraoxonase 1 (PON1) have been associated with the development of several pathological conditions, whereas high levels have been shown to be anti-atherosclerotic in mouse models. These findings suggest that PON1 could be a good surrogate biomarker. The other members of the family, namely PON2 and PON3, the role of which has been much less studied, deserve more attention. This paper provides a systematic review of current evidence concerning dietary supplements in that regard. Preliminary studies indicate that the response to dietary supplements may have a nutrigenetic aspect that will need to be considered in large population studies or in clinical trials. A wide range of plant preparations have been found to have a positive action, with pomegranate and some of its components being the best characterized and Aronia melanocarpa one of the most active. Flavonoids are found in the composition of all active extracts, with catechins and genistein being the most promising agents for increasing PON1 activity. However, some caveats regarding the dose, length of treatment, bioavailability, and stability of these compounds in formulations still need to be addressed. Once these issues have been resolved, these compounds could be included as nutraceuticals and functional foods capable of increasing PON1 activity, thereby helping with the long-term prevention of atherosclerosis and other chronic ailments.

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“…But more recently research has been focused on other clinical aspects such as protective role in vascular disease as well as its use as biomarker of diseases involving mainly three situations: (a) oxidative stress, since PON1 protects against oxidation [8]; (b) inflammation, being considered PON1 as a negative acute phase protein [9] and (c) liver diseases, because PON1 is synthesized in this organ [10]. In veterinary medicine, studies of PON1 have been traditionally focused in bovine [11,12], however in recent years it has gained interest in other species such as dogs [13,14], cats [15] and horses [16]. …”

Section: Introductionmentioning

confidence: 99%

Serum paraoxonase 1 (PON1) measurement: an update

2014

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Paraoxonase 1 (PON1) is a widely studied enzyme based on its protective role against poisoning by organophosphate (OP) metabolites of specific OP insecticides and in vascular disease, as well as its use as biomarker of diseases involving oxidative stress, inflammation and liver diseases.This review provides an update about the current knowledge in the field of the analytical procedures that are used for PON1 measurements. It will be specially focused on: (a) characteristics of the different substrates used for measuring PON1, with emphasis in four aspects: toxicity, polymorphism influence, rate of hydrolysis and diagnostic performance. And (b) the technical aspects of PON1 assays, in which the reagents and reaction conditions, sources of variation, quality control systems, equipment and interferences with other esterases will be discussed.The information provided in this review can contribute to a more accurate and safe measurements of PON1 in laboratories and encourage researchers to explore the wide areas of PON1 in veterinary medicine that are still unknown.

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Protecting effect of vitamin E supplementation on submaximal exercise-induced oxidative stress in sedentary dogs as assessed by erythrocyte membrane fluidity and paraoxonase-1 activity

Cited by 24 publications

References 43 publications

Erythrocyte membrane fluidity and indices of plasmatic oxidative damage after acute physical exercise in humans

Erythrocyte membrane fluidity and indices of plasmatic oxidative damage after acute physical exercise in humans

The Search for Dietary Supplements to Elevate or Activate Circulating Paraoxonases

Serum paraoxonase 1 (PON1) measurement: an update

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