Proteasome Inhibitors Affect Appressorium Formation and Pathogenicity of the Rice Blast Fungus, Magnaporthe oryzae

Wang, Yiming; Kim, Pil Joo; Wu, Jingni; Yu, Seok‐Ho; Kang, Kyu Young; Kim, Sun Tae

doi:10.5423/ppj.2011.27.3.225

Cited by 10 publications

(7 citation statements)

References 33 publications

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“…Similar upregulation of metabolic pathways has been observed at the transcriptional level associated with conidial germination in dikaryan fungi (Sharma et al, 2016;Zhou et al, 2018). Interestingly, we detected the upregulation of proteasome genes in the germling relative to the zoospore, which are also necessary for dikaryan germination (Seong et al, 2008;Wang et al, 2011). A previous study into flagellar retraction in R. globosum showed that the internalised flagellum is disassembled and degraded in the germling stage, at least partially by proteasome-dependent proteolysis (Venard et al, 2020).…”

Section: Discussionsupporting

confidence: 79%

A cellular and molecular atlas reveals the basis of chytrid development

Laundon

Chrismas

Bird

et al. 2021

Preprint

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The chytrids (phylum Chytridiomycota) are a major early-diverging fungal lineage of ecological and evolutionary importance. Despite their importance, many fundamental aspects of chytrid developmental and cell biology remain poorly understood. To address these knowledge gaps, we combined quantitative volume electron microscopy and comparative transcriptome profiling to create an "atlas" of the cellular and molecular basis of the chytrid life cycle, using the model chytrid Rhizoclosmatium globosum. From our developmental atlas, we show that zoospores exhibit a specialised biological repertoire dominated by inactive ribosome aggregates, and that lipid processing is complex and dynamic throughout the cell cycle. We demonstrate that the chytrid apophysis is a distinct subcellular structure characterised by high intracellular trafficking, providing evidence for division of labour in the chytrid cell plan, and show that zoosporogenesis includes "animal like" amoeboid cell morphologies resulting from endocytotic cargo transport from the interstitial maternal cytoplasm. Taken together, our results reveal insights into chytrid developmental biology and provide a basis for future investigations into early-diverging fungal cell biology.

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Section: Discussionsupporting

confidence: 79%

A cellular and molecular atlas reveals the basis of chytrid development

Laundon

Chrismas

Bird

et al. 2021

Preprint

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“…Moreover, addition of Bortezomib to inoculum blocked symptom development, even though melanized appressoria were formed after 48 hr. A recent report, which was published during the course of this research, also showed the proteasome inhibitors MG-132, proteasome inhibitor I and proteome inhibitor II delayed conidia germination and appressorium formation in M. oryzae as well as suppressed infection of rice leaves [32] .…”

Section: Discussionmentioning

confidence: 54%

Polyubiquitin Is Required for Growth, Development and Pathogenicity in the Rice Blast Fungus Magnaporthe oryzae

Franck

Han

et al. 2012

PLoS ONE

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Protein ubiquitination, which is highly selective, regulates many important biological processes including cellular differentiation and pathogenesis in eukaryotic cells. Here, we integrated pharmacological, molecular and proteomic approaches to explore the role of ubiquitination in Magnaporthe oryzae, the leading fungal disease of rice world-wide. Inhibition of ubiquitin-mediated proteolysis using the 26S proteasome inhibitor, Bortezomib, significantly attenuated conidia germination, appressorium formation and pathogenicity in M. oryzae. Gene expression analysis revealed that many genes associated with protein ubiquitination were developmentally regulated during conidia germination. Only a few, including a polyubiquitin encoding gene, MGG_01282, were more abundantly expressed during appressorium formation and under nitrogen starvation. Targeted gene deletion of MGG_01282, in addition to a significant reduction in protein ubiquitination as determined by immuno blot assays, resulted in pleiotropic effects on M. oryzae including reduced growth and sporulation, abnormal conidia morphology, reduced germination and appressorium formation, and the inability to cause disease. Mutants were also defective in sexual development and were female sterile. Using mass spectrometry, we identified 63 candidate polyubiquitinated proteins under nitrogen starvation, which included overrepresentation of proteins involved in translation, transport and protein modification. Our study suggests that ubiquitination of target proteins plays an important role in nutrient assimilation, development and pathogenicity of M. oryzae.

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“…The upregulation of this protein has been reported in the germination of Nosema bombycis spores (Liu et al, 2016) and might indicate a large turnover of proteins associated with the germination of resting spores (Seong et al, 2008). Wang et al (2011) showed that proteasome inhibitors can affect the pathogenicity of Magnaporthe oryzae, the rice blast fungus, and delayed its spore formation as well.…”

Section: Discussionmentioning

confidence: 88%

“…Wang et al . (2011) showed that proteasome inhibitors can affect the pathogenicity of Magnaporthe oryzae , the rice blast fungus, and delayed its spore formation as well.…”

Section: Discussionmentioning

confidence: 99%

Quantitative proteomics provides an insight into germination‐related proteins in the obligate biotrophic plant pathogen Spongospora subterranea

Balotf

Wilson

Tegg

et al. 2021

Environ Microbiol Rep

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The soil-borne and obligate plant-associated nature of S. subterranea has hindered a detailed study of this pathogen and in particular, the regulatory pathways driving the germination of S. subterranea remain unknown. To better understand the mechanisms that control the transition from dormancy to germination, protein profiles between dormant and germination stimulant-treated resting spores were compared using label-free quantitative proteomics. Among the $680 proteins identified 20 proteins were found to be differentially expressed during the germination of S. subterranea resting spores. Elongation factor Tu, histones (H2A and H15), proteasome and DJ-1_PfpI, involved in transcription and translation, were upregulated during the germination of resting spores. Downregulation of both actin and betatubulin proteins occurred in the germinating spores, indicating that the changes in the cell wall cytoskeleton may be necessary for the morphological changes during the germination of the resting spore in S. subterranea. Our findings provide new approaches for the study of these and similar recalcitrant microorganisms provide the first insights into the basic protein components of S. subterranea spores. A better understanding of S. subterranea biology may lead to the development of novel approaches for the management of persistent soil inoculum.

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Proteasome Inhibitors Affect Appressorium Formation and Pathogenicity of the Rice Blast Fungus, Magnaporthe oryzae

Cited by 10 publications

References 33 publications

A cellular and molecular atlas reveals the basis of chytrid development

A cellular and molecular atlas reveals the basis of chytrid development

Polyubiquitin Is Required for Growth, Development and Pathogenicity in the Rice Blast Fungus Magnaporthe oryzae

Quantitative proteomics provides an insight into germination‐related proteins in the obligate biotrophic plant pathogen Spongospora subterranea

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