Proteasome and C-reactive protein inflammatory response in children undergoing shorter and longer lasting laparoscopic cholecystectomy

Abstract

Operations of varying duration cause the release of a number of inflammatory mediators, in particular cytokines which lead to proteasome and acute-phase reactions. The purpose of this novel human study, was to characterize inflammatory response in children undergoing laparoscopic cholecystectomy, by analyzing changes in selected inflammatory mediators: C-reactive protein concentration and circulating 20S proteasome activity following surgical injury and to correlate them with the duration of the surgical procedure. Plasma C-reactive protein concentration (CRP) was determined by standard biochemical laboratory procedures. Proteasome activity in the plasma of children was assessed using Suc-Leu-Leu-Val-Tyr-AMC peptide substrate. Statistically significant increase in the plasma proteasome activity and C-reactive protein concentration, was noted (p < .05) in children after laparoscopic cholecystectomy. We found the correlation between the 20S proteasome activity and the length of the procedure. In children undergoing longer lasting laparoscopic cholecystectomy the proteasome activity was much higher than in patients having shorter surgical procedure. The CRP concentration and 20S proteasome activity significantly increase after surgery, but only 20S proteasome activity correlate with the length of the surgery. This may confirm that CRP is only an indicator of pathological state, while the function of the proteasomes is more complex because of their participation in the processes of repair and wound healing, and in the removal of damaged proteins.

The Concentration of Selected Inflammatory Cytokines (IL-6, IL-8, CXCL5, IL-33) and Damage-Associated Molecular Patterns (HMGB-1, HSP-70) Released in an Early Response to Distal Forearm Fracture and the Performed Closed Reduction With Kirschner Wire Fixation in Children

“…CRP concentration may reflect the development and augmentation of an inflammatory response, as we showed in several of our previous studies (19,(31)(32)(33). In the current study, we noted statistically significant differences in CRP concentration between patients 30 min.…”

Proteasome Activity and C-Reactive Protein Concentration in the Course of Inflammatory Reaction in Relation to the Type of Abdominal Operation and the Surgical Technique Used

“…Our earlier findings showed that the proteasome may be a biomarker of tissue damage—more severe in the group of burn patients in comparison to patients with mild head injury and blunt abdominal trauma [ 34 , 35 ]. Also, the last study showed that correlation between proteasome activity and length of surgery in contrast to the C-reactive protein indicated that CRP is only an indicator of the pathological state, while the function of the proteasome is more complex due to its participation in the oxidative stress-induced repair of tissue damage [ 16 ].…”

“…The proteasome chymotrypsin-like activity in the plasma was assayed using the fluorogenic peptide substrate Suc-Leu-Leu-Val-Tyr-AMC in the presence of SDS (sodium dodecyl sulfate-selective enzyme activator), as described by Ma et al and Tylicka et al [ 15 , 16 ]. The 20S proteasome cannot efficiently degrade peptides or proteins unless they are highly denatured; therefore, the 20S proteasome in plasma has been activated by the addition of sodium dodecyl sulfate.…”

Intraoperative Peritoneal Interleukin-6 Concentration Changes in Relation to the High-Mobility Group Protein B1 and Heat Shock Protein 70 Levels in Children Undergoing Cholecystectomy

“…Cholecystectomy is a common surgery performed using the open or laparoscopic approach. It is established that the laparoscopic cholecystectomy is more beneficial compared to the open surgery procedure [1][2][3][4]. On the other hand, in the available literature, there are some voices indicating laparoscopic intervention as equivalent or even inferior compared to open surgery [5].…”