Proteasomal degradation pathways in Trametes versicolor and Phlebia radiata

Staszczak, Magdalena

doi:10.1016/s0141-0229(02)00010-8

Cited by 21 publications

(15 citation statements)

References 24 publications

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“…Por lo tanto, aireación, presencia de oxigeno y dosis adecuada de glucosa permiten la degradación de la lignina al cabo de 7 días, previo consumo de la glucosa (Lobarzewski y Paszczynski 1985, Olvera 2003. También es de notar que las enzimas son expresadas durante la fase secundaria del crecimiento (idiofase) cuando la limitación de carbono, nitrógeno y sulfuro ocurre (Staszczak, 2002); sin embargo es necesario realizar estudios para evaluar que tipo de enzima esta actuando.…”

Section: Discussionunclassified

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Hongos filamentosos con actividades ligninolíticas aislados de Calamagrostis nitidula Pilg.

Laura

Castellanos

2011

Rev peru biol

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Hongos con actividad ligninolitica fueron aislados de la planta forrajera Calamagrostis nitidula Pilg. (Poaceae). Se utilizo el medio mínimo Czapeck conteniendo lignina (0,2%) como única fuente de carbono, los medios fueron inoculados con fracciones de C. nitidula. Posteriormente los hongos desarrollados fueron aislados en agar papa dextrosa (APD) e identificados según sus características macroscópicas y microscópicas. Se aislaron e identificaron los siguientes géneros: Alternaria, Ulocladium, Trichoderma, Cephalosporium, Helicomyces, Mucor y Aspergillus. La capacidad de degradación de lignina fue determinada cualitativamente y cuantitativamente, inoculando cada cepa obtenida en tubos con caldo Czapeck con 0,2% de lignina y 1% de glucosa. La cepa PD5F identificada como Aspergillus melleus Yukawa fue la única con actividad ligninolitica.Palabras claves: Degradación ligninolítica, Calamagrostis nitidula, Aspergillus melleus, lignina, ligninasas. AbstractWe isolated fungi with ligninolytic activity of the forage plant Calamagrostis nitidula Pilg. (Poaceae). The minimum Czapeck medium containing lignin (0,2%) as carbon source, was inoculated with fractions of C. nitidula. Subsequently, the fungus developed were isolated in potato dextrose agar (PDA) and identified according to their macroscopic and microscopic characteristics. The following genus were isolated and identified: Alternaria, Ulocladium, Trichoderma, Cephalosporium, Helicomyces Mucor and Aspergillus. Degradation of lignin was determined qualitatively and quantitatively, by inoculating each strain obtained in tubes with Czapeck medium with 0,2% lignin and 1% glucose. Only the PD5F strain identified as Aspergillus melleus Yukawa was ligninolytic activity.

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Section: Discussionunclassified

“…Se ha señalado que para que se active las enzima MnP y la lacasa tienen que tener concentraciones de Mn(II) y Cu minimas, en caso contrario no se detectaría la actividad de estas isoenzimas (Polanco et al 2002, Staszczak 2002.…”

Section: Discussionunclassified

Hongos filamentosos con actividades ligninolíticas aislados de Calamagrostis nitidula Pilg.

Laura

Castellanos

2011

Rev peru biol

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“…Trametes versicolor (ATCC 44308) was grown as described previously (Staszczak, 2002). Briefly, mycelia were maintained as surface cultures at 26°C, in flasks containing 10 ml of nutrient-rich growth medium (Fahraeus and Reinhammar, 1967).…”

Section: Fungal Strains and Culture Conditionsmentioning

confidence: 99%

“…Crude mycelial extracts were prepared essentially as described previously (Staszczak, 2002). Briefly, mycelia were harvested and homogenized in an ice-chilled motordriven Potter's homogenizer, in 2 ml of 50 mM Tris-HCl buffer, pH 7.3, containing 5 mM MgCl 2 .…”

Section: Preparation Of the Extracellular Culture Fluid And Mycelium mentioning

confidence: 99%

“…These 26S proteasomes were the first ones reported in fungi other than yeast. Preliminary in vivo experiments performed in our laboratory using one of the most potent peptide aldehyde inhibitors of the proteasome, MG 132 (Z-Leu-Leu-Leu-al), to block proteasome function in T. versicolor and P. radiata strongly suggested the involvement of proteasome-mediated proteolytic pathway in the regulation of ligninolytic activities upon nitrogen-and carbon-starvation (Staszczak, 2002). Subsequent studies carried out with T. versicolor demonstrated that degradation of intracellular proteins by 26S proteasomes played an important role in the regulation of laccase activity in response to Cd 2+ exposure; blocking proteasomes by MG 132 and lactacystin b-lactone (a more selective inhibitor of proteasomes) strongly affected Cd 2+ -stimulated laccase activity in Cd 2+ -supplemented cultures of the fungus (Staszczak and Jarosz-Wilkołazka, 2005).…”

Section: Introductionmentioning

confidence: 99%

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The role of the ubiquitin–proteasome system in the response of the ligninolytic fungus Trametes versicolor to nitrogen deprivation

Staszczak

2008

Fungal Genetics and Biology

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Proteomic analysis of the phytopathogenic soilborne fungusVerticillium dahliaereveals differential protein expression in isolates that differ in aggressiveness

2010

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Verticillium dahliae is a soilborne fungus that causes a vascular wilt disease of plants and losses in a broad range of economically important crops worldwide. In this study, we compared the proteomes of highly (Vd1396-9) and weakly (Vs06-14) aggressive isolates of V. dahliae to identify protein factors that may contribute to pathogenicity. Twenty-five protein spots were consistently observed as differential in the proteome profiles of the two isolates. The protein sequences in the spots were identified by LC-ESI-MS/MS and MASCOT database searches. Some of the identified sequences shared homology with fungal proteins that have roles in stress response, colonization, melanin biosynthesis, microsclerotia formation, antibiotic resistance, and fungal penetration. These are important functions for infection of the host and survival of the pathogen in soil. One protein found only in the highly aggressive isolate was identified as isochorismatase hydrolase, a potential plant-defense suppressor. This enzyme may inhibit the production of salicylic acid, which is important for plant defense response signaling. Other sequences corresponding to potential pathogenicity factors were identified in the highly aggressive isolate. This work indicates that, in combination with functional genomics, proteomics-based analyses can provide additional insights into pathogenesis and potential management strategies for this disease.

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Proteasomal degradation pathways in Trametes versicolor and Phlebia radiata

Cited by 21 publications

References 24 publications

Hongos filamentosos con actividades ligninolíticas aislados de Calamagrostis nitidula Pilg.

Hongos filamentosos con actividades ligninolíticas aislados de Calamagrostis nitidula Pilg.

The role of the ubiquitin–proteasome system in the response of the ligninolytic fungus Trametes versicolor to nitrogen deprivation

Proteomic analysis of the phytopathogenic soilborne fungusVerticillium dahliaereveals differential protein expression in isolates that differ in aggressiveness

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