Proteases in Mosquito Borne Diseases: New Avenues in Drug Development

Pant, A.K.; Pasupureddy, Rahul; Pande, Veena; Seshadri, Sriram; Dixit, Rajnikant; Pandey, Kailash C.

doi:10.2174/1568026617666170130122231

Cited by 4 publications

(2 citation statements)

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“…The location of NS2B on the membrane is critical for protease activity, which makes NS3 perform its other functions (important for viral replication). Therefore, both NS2B and NS3 are important targets for developing antivirals [88].…”

Section: Protease Structure and Dynamicsmentioning

confidence: 99%

Structure and Dynamics of Zika Virus Protease and Its Insights into Inhibitor Design

Kang

2021

Biomedicines

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Zika virus (ZIKV)—a member of the Flaviviridae family—is an important human pathogen. Its genome encodes a polyprotein that can be further processed into structural and non-structural proteins. ZIKV protease is an important target for antiviral development due to its role in cleaving the polyprotein to release functional viral proteins. The viral protease is a two-component protein complex formed by NS2B and NS3. Structural studies using different approaches demonstrate that conformational changes exist in the protease. The structures and dynamics of this protease in the absence and presence of inhibitors were explored to provide insights into the inhibitor design. The dynamic nature of residues binding to the enzyme cleavage site might be important for the function of the protease. Due to the charges at the protease cleavage site, it is challenging to develop small-molecule compounds acting as substrate competitors. Developing small-molecule compounds to inhibit protease activity through an allosteric mechanism is a feasible strategy because conformational changes are observed in the protease. Herein, structures and dynamics of ZIKV protease are summarized. The conformational changes of ZIKV protease and other proteases in the same family are discussed. The progress in developing allosteric inhibitors is also described. Understanding the structures and dynamics of the proteases are important for designing potent inhibitors.

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Section: Protease Structure and Dynamicsmentioning

confidence: 99%

Structure and Dynamics of Zika Virus Protease and Its Insights into Inhibitor Design

Kang

2021

Biomedicines

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“…Most exported proteins must traverse two membranes to their destination in the infected erythrocyte cytosol, the parasite plasma membrane and surrounding parasitophorous vacuole membrane. Interactions and cleavage of PEXEL proteins by plasmepsin V is necessary for export, which in turn is required for the erythrocytic stage development of malaria parasites [1,2]. This makes plasmepsin V an attractive antimalarial drug target, butas with all inhibitors of pathogen proteases -the candidate compounds must be highly specific.…”

Section: Wo/2017/089453mentioning

confidence: 99%