Protease production by immobilized growing cells of Serratia marcescens and Myxococcus xanthus in calcium alginate gel beads

Vuillemard, Jean-Christophe; Terre, S.; Benoit, Stéphane L.; Amiot, Jean

doi:10.1007/bf00451607

Cited by 41 publications

(15 citation statements)

References 11 publications

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“…These findings were in accordance with those obtained previously for the protease production by immobilized Serratia marcescens and Myxococcus xanthus in calcium alginate beads. 29 It was found that protease production by immobilized S marcescens increased with repeated growth cycles, and reached a maximum after 5 cycles. Bandyopadhyay et al 30 studied erythromycin production by Streptomyces erythreus entrapped in calcium alginate beads and obtained efficient productivity of erythromycin.…”

Section: Repeated Batch Fermentation With Free Cells and Immobilized mentioning

confidence: 98%

Production of alkaline protease with immobilized cells of bacillus subtilis PE-11 in various matrices by entrapment technique

2005

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The purpose of this investigation was to study the effect of Bacillus subtilis PE-11 cells immobilized in various matrices, such as calcium alginate, k-Carrageenan, ployacrylamide, agar-agar, and gelatin, for the production of alkaline protease. Calcium alginate was found to be an effective and suitable matrix for higher alkaline protease productivity compared to the other matrices studied. All the matrices were selected for repeated batch fermentation. The average specific volumetric productivity with calcium alginate was 15.11 U/mL/hour, which was 79.03% higher production over the conventional free-cell fermentation. Similarly, the specific volumetric productivity by repeated batch fermentation was 13.68 U/mL/hour with k-Carrageenan, 12.44 U/mL/hour with agar-agar, 11.71 U/mL/hour with polyacrylamide, and 10.32 U/mL/hour with gelatin. In the repeated batch fermentations of the shake flasks, an optimum level of enzyme was maintained for 9 days using calcium alginate immobilized cells. From the results, it is concluded that the immobilized cells of B subtilis PE-11 in calcium alginate are more efficient for the production of alkaline protease with repeated batch fermentation. The alginate immobilized cells of B subtilis PE-11 can be proposed as an effective biocatalyst for repeated usage for maximum production of alkaline protease.

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Section: Repeated Batch Fermentation With Free Cells and Immobilized mentioning

confidence: 98%

Production of alkaline protease with immobilized cells of bacillus subtilis PE-11 in various matrices by entrapment technique

2005

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“…The cells in the control culture grew as small pellets and some cells immobilization (Younes et al, 1984(Younes et al, , 1987. The increase we obtained was much less than for proteolytic activity which increased eight-fold (Vuillemard et al, 1988).…”

Section: Resultsmentioning

confidence: 87%

“…A variety of methods exist for immobilizing microbial cells including ionic or covalent binding to water insoluble ion exchangers, adsorption onto a solid surface and by entrapment in a polymer matrix (Hattori andFurasaka, 1960, 1961;Mosbach and Larson, 1970;Chibata et al, 1974, Chibata andTosa, 1977;Chibata, 1978). Production of extracellular proteolytic enzymes by Myxococcus xanthus has been shown to be enhanced by immobilization (Younes et al, 1984(Younes et al, , 1987Vuillemard et al, 1988) but there are no reports of the effects on antibiotic production. We therefore investigated the effects of immobilization of myxobacteria on antibiotic production.…”

Section: Introductionmentioning

confidence: 99%

Improved yield of antibiotics by immobilization of cells of myxobacteria

Yasouri

Foster

1991

Biotechnol Tech

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“…While proteinases production by free cells has been extensively studied, only a few publications with fungal immobilized cells (IC) in shake flasks have been reported. Most of the immobilized fungal microorganisms have been entrapped in Caalginate (8,11,4); in polyacrylamide (5,3); carrageenan (9,10), polyurethane sponge (2) and poly HEMA-EDMA (1).…”

Section: Introductionmentioning

confidence: 99%

Repeated Batch Acid Proteinases Biosyntesis by Immobilized Humicola Lutea Cells Cultivated in Bioreactor

Алексиева

Manolov

Petricheva

et al. 1995

Biotechnology & Biotechnological Equipment

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Fungal strain Humicola lutea 120-5 was immobilized in poly[hydroxyethylmethacrylate (HEMA)-ethyleneglycoldimethacrylate (EDMA)J gel. Immobilized cells were cultivated in column and airlift bioreactors with different height to diameter (HID) ratio for acid proteinases production during batch and repeated-batch processes. Influence of aeration rate in the bioreactors on enzyme production was studied. Optimal conditions for maximum proteinases production were obtained during repeated batch cultivation of immobilized cells (JC) in an airlift bioreactor with HID=4 and aeration rate of 6 wm.

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Protease production by immobilized growing cells of Serratia marcescens and Myxococcus xanthus in calcium alginate gel beads

Cited by 41 publications

References 11 publications

Production of alkaline protease with immobilized cells of bacillus subtilis PE-11 in various matrices by entrapment technique

Production of alkaline protease with immobilized cells of bacillus subtilis PE-11 in various matrices by entrapment technique

Improved yield of antibiotics by immobilization of cells of myxobacteria

Repeated Batch Acid Proteinases Biosyntesis by Immobilized Humicola Lutea Cells Cultivated in Bioreactor

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