Protease inhibitor regulation of B-cell differentiation

Arora, Prince K.; Miller, Harold C.; Aronson, Lawrence D.

doi:10.1016/0008-8749(81)90256-2

Cited by 11 publications

(4 citation statements)

References 29 publications

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“…Cell viability was evaluated with the trypan blue exclusion test and determined to be greater than 80%. The heterogeneous spleen cell population has been reported to consist of mostly lymphocytes, e.g., T and B cells [20,21].…”

Section: Splenocyte Isolationmentioning

confidence: 99%

Sleep deprivation impairs calcium signaling in mouse splenocytes and leads to a decreased immune response

Lungato

Gazarini

Paredes‐Gamero

et al. 2012

Biochimica et Biophysica Acta (BBA) - General Subjects

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Section: Splenocyte Isolationmentioning

confidence: 99%

Sleep deprivation impairs calcium signaling in mouse splenocytes and leads to a decreased immune response

Lungato

Gazarini

Paredes‐Gamero

et al. 2012

Biochimica et Biophysica Acta (BBA) - General Subjects

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“…The spleen cell population is heterogeneous, consisting mostly of lymphocytes, i.e. Tand B-cells (Arora et al 1981), which play an important role in cellular and humoral immunity, respectively.…”

Section: Introductionmentioning

confidence: 99%

Acute restraint stress enhances calcium mobilization and proliferative response in splenic lymphocytes from mice

Satoh

Edamatsu

Omata

2006

Stress

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Calcium (Ca2+ ) plays an essential role in lymphocyte activation and maturation. Acute and chronic stress has been shown to modulate the lymphocyte immune response; but the relationship between cytosolic free Ca2+ concentration ([Ca2+ ]i) and the immune response in lymphocytes following exposure to stress has not been examined. In the present study, we investigated the effects of acute restraint stress on [Ca2+ ]i and the proliferation of splenic lymphocytes from mice. We observed that 2 h of restraint significantly increased plasma corticosterone levels in mice. On examining [Ca2+ ]i and the proliferation ex vivo of splenic lymphocytes isolated from restraint-stressed mice using fura-2 and 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide, respectively, we found that acute restraint stress caused a significant increase in resting [Ca2+ ]i and significantly enhanced the ability of concanavalin A (Con A; a T-cell-selective mitogen) to increase [Ca2+ ]i but not that of lipopolysaccharide (LPS; a B-cell-selective mitogen). In addition, acute restraint stress significantly enhanced Con A-stimulated but not LPS-stimulated lymphocyte proliferation. Overall, there was a positive correlation between [Ca2+ ]i and T-cell proliferation following acute restraint stress. The enhancements of [Ca2+ ]i and T-cell proliferation were completely suppressed by verapamil (a Ca2+ channel blocker). These results suggest that acute restraint stress enhances Con A-stimulated T-cell proliferation by increasing [Ca2+ ]i via stimulation of Ca2+ entry.

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“…The spleen cell population is heterogeneous, consisting mostly of lymphocytes, i.e. T-and B-lymphocytes [16], with the former and latter playing an important role in cellular and humoral immunity, respectively.…”

Section: Introductionmentioning

confidence: 99%

Experimental diabetes attenuates calcium mobilization and proliferative response in splenic lymphocytes from mice

Satoh

Iwasaki

2010

J Physiol Sci

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The present study was conducted to investigate the effects of the diabetic condition on cytosolic free Ca(2+) concentration, [Ca(2+)](i), and the proliferation of splenic lymphocytes from mice. Diabetes was induced in mice by intraperitoneal injection of alloxan. [Ca(2+)](i) and the proliferation ex vivo of splenic lymphocytes isolated from mice were examined using fura-2 and 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide, respectively. Diabetes caused a significant increase in resting [Ca(2+)](i) and significantly reduced the ability of concanavalin A (Con A; a T-lymphocyte-selective mitogen) to increase [Ca(2+)](i), but not that of lipopolysaccharide (LPS; a B-lymphocyte-selective mitogen). In addition, diabetes significantly reduced Con A-stimulated but not LPS-stimulated lymphocyte proliferation. Verapamil (an L-type Ca(2+) channel blocker) inhibited Con A-induced increases in [Ca(2+)](i) and proliferation in lymphocytes from control and diabetic mice to a similar extent, respectively. These results suggest that diabetes attenuates Con A-stimulated T-lymphocyte proliferation by decreasing [Ca(2+)](i) via reduction of Ca(2+) entry through L-type Ca(2+) channels.

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Protease inhibitor regulation of B-cell differentiation

Cited by 11 publications

References 29 publications

Sleep deprivation impairs calcium signaling in mouse splenocytes and leads to a decreased immune response

Sleep deprivation impairs calcium signaling in mouse splenocytes and leads to a decreased immune response

Acute restraint stress enhances calcium mobilization and proliferative response in splenic lymphocytes from mice

Experimental diabetes attenuates calcium mobilization and proliferative response in splenic lymphocytes from mice

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