Protease activity and invasion of matrigel by the osteosarcoma-derived OSPR cell line

Yan, Lin; Kumagai, S; McGuire, Michael H.; Yee, John A.

doi:10.1042/bst022018s

Cited by 2 publications

(2 citation statements)

References 3 publications

(3 reference statements)

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“…For the evaluation of invasiveness of tumor in vitro, we performed Matrigel invasion assay, which has been commonly used to test cell invasion activity (22)(23)(24). We found that both 3 and 10 µM of MMP-9-NF-κB PIP restrained cell invasiveness significantly in comparison to control.…”

Section: Discussionmentioning

confidence: 99%

Inhibition of MMP-9 using a pyrrole-imidazole polyamide reduces cell invasion in renal cell carcinoma

Sato¹,

Nagase²,

Obinata³

et al. 2013

International Journal of Oncology

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Abstract.We investigated the clinical significance of the expression levels of matrix metalloproteinase 9 (MMP-9) in renal cell carcinoma (RCC). In addition, we validated the efficacy of pyrrole imidazole polyamide (PIP) targeting MMP-9 on inhibiting proliferation and invasion of RCC. We evaluated the expression levels of MMP-9 in 249 RCC specimens by immunostaining and analyzed the association between MMP-9 expression levels and cancer-specific survival. Furthermore, in a human RCC cell line, Caki-2, we tested the effect of a couple of PIPs targeting MMP-9 one recognizing an NF-κB binding site (MMP-9-NF-κB PIP) and another for the AP-1 binding site (MMP-9-AP-1 PIP) in the MMP-9 promoter. The expression levels of MMP-9, proliferative activity and invasive capability were tested by quantitative PCR, WST8 assay and Matrigel invasion assay, respectively. By immunostaining of the clinical specimens, strong MMP-9 staining was proven to be a significant predictor of poor prognosis for cancer-specific survival (P<0.01). In Caki-2 cells, MMP-9-NF-κB PIP significantly reduced the expression levels of MMP-9 mRNA and inhibited cell invasion, but did not affect the cell proliferation activity. On the other hand, no effect was found in MMP-9-AP-1 PIP on MMP-9 mRNA expression, cell proliferation and invasion. We confirmed the inhibitory effects of MMP-9-NF-κB PIP on the expression of MMP-9 and subsequent invasion of Caki-2 cells. Since it was clearly shown that high MMP-9 expression levels were associated with poor prognosis of RCC, MMP-9 is a potential candidate target for RCC treatment. Transcription therapy using a minor groove binder, such as NF-κB PIP, may be a potential therapeutic agent for RCC, although further investigation is required.

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Section: Discussionmentioning

confidence: 99%

Inhibition of MMP-9 using a pyrrole-imidazole polyamide reduces cell invasion in renal cell carcinoma

Sato¹,

Nagase²,

Obinata³

et al. 2013

International Journal of Oncology

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“…[52–56] Clinical studies reveal that measuring FDP levels, either with the DR-70 (FDP) test or with other related tests, has significant diagnostic value for a variety of cancers. These studies demonstrate that FDP levels correlate with the cancer stage [41–43,57–60] and with the cancer progression, [41,59,61] as quantified by the number of lymph node metastases.…”

Section: Discussionmentioning

confidence: 99%

Advantages of the Amdl-Elisa DR-70 (Fdp) Assay Over Carcinoembryonic Antigen (Cea) for Monitoring Colorectal Cancer Patients

Small‐Howard¹,

Harris²

2010

Journal of Immunoassay and Immunochemistry

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The DR-70® (FDP) test was the first cancer test cleared by USFDA for monitoring colorectal cancer (CRC) since Carcinoembryonic Antigen (CEA) in 1982. Conservatively, 50% of biopsy-positive CRC patients have negative CEA values. DR-70 and CEA values were compared for 113 CRC monitoring patients. Total concordance rates for DR-70 and CEA were 0.665 and 0.686, respectively. CRC patient pairs were grouped based on their CEA value to deduce DR-70's effectiveness at monitoring patients with low CEA values. DR-70 had 12% to 100% greater positive concordance rates than CEA in this group. DR-70 is a welcome new option for CRC patients.

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Protease activity and invasion of matrigel by the osteosarcoma-derived OSPR cell line

Cited by 2 publications

References 3 publications

Inhibition of MMP-9 using a pyrrole-imidazole polyamide reduces cell invasion in renal cell carcinoma

Inhibition of MMP-9 using a pyrrole-imidazole polyamide reduces cell invasion in renal cell carcinoma

Advantages of the Amdl-Elisa DR-70 (Fdp) Assay Over Carcinoembryonic Antigen (Cea) for Monitoring Colorectal Cancer Patients

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