Prostatik Adenokarsinomlarda Triptaz ve Kimaz Ekspresyon Farklılıkları

Abstract

Objective Mast cells (MCs) have been shown to have various roles in many tumors. MCs play roles in tumor progression via increasing angiogenesis and lymphangiogenesis. According to the presence of tryptase and chymase granules, mast cells are two varieties containing tryptasechymase and containing tryptase. The aim of this study was to investigate the presence and distribution of these two mast cell types in PCa in terms of their tryptase and chymase content. ( Sakarya Med J, 2018, 8(2):229-234 ) Materials and MethodsIn this study, immunohistochemical analysis of tryptase and chymase expression was performed retrospectively in 134 patients who have diagnosed PCa histopathologically, 2012 and 2014. Immunohistochemisrty experiment was done on the appropriate paraffi n blocks by using mast cell tryptase and chymase antibodies. The research fi ndings were evaluated statistically and accepted as P <0.05 level.Results It has been observed that Mast Cell Chymase (MCTC) accumulates in intratumoral areas more than peritumoral areas (respectively; mean: 14,28 ± 14,06, mean: 12,12 ± 12,56). Mast Cell Tryptase (MCT) accumulates more than MCTC in intratumoral and peritumoral areas (P<0,001). MCT was observed to be more frequent in peritumoral areas than in intratumoral areas (respectively; mean: 33,94 ± 20,09, mean:33,50 ± 18,65). MCT and MCTC compared with regard to intratumoral and peritumoral areas. There wasn't a signifi cant difference with MCT (p=0.723). However, MCTC was found the intratumoral area more than the peritumoral area (p=0.007).Conclusion Mast cells numbers were increased around prostatic adenocarcinoma microenvironment. MCTC was tended to locate intratumoral whereas MCT was tended to the located peritumoral area. Note: This article was presented as a poster at the 27th European Congress of Pathology 5 to 9 September 2015 in Belgrade, Serbia. Öz Amaç Mast hücrelerinin (MCs) birçok tümörde çeşitli rolleri olduğu gösterilmiştir. MCs, artan anjiyogenez ve lenfogenez yoluyla tümör progresyonunda rol oynamaktadır. Triptaz ve kimaz granüllerinin varlığına göre mast hücreleri; triptaz-kimaz içeren ve triptaz içeren olmak üzere iki çeşittir. Bu çalışmanın amacı, içeriklerine göre farklı bu iki mast hücre tipinin prostatik adenokarsinomalardaki (PCa) varlığı ve dağılımını araştırmaktır.( Sakarya Tıp Dergisi, 2018, 8(2):229-234 ).