Prostatic luminal cell differentiation and prostatic steroid‐binding protein (PBP) gene expression are differentially affected by neonatal castration

Janulis, Lynn; Nemeth, Jeffrey A.; Yang, Tony T.; Lang, Sharon; Lee, Chung

doi:10.1002/(sici)1097-0045(20000515)43:3<195::aid-pros5>3.0.co;2-#

Cited by 4 publications

(3 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This may be an indirect consequence of the MEHP-induced decrease in testosterone production, as Pbp levels have also been shown to be decreased by castration in the rat [49] and by exposure to other antiandrogenic agents, such as prochloraz or flutamide, in castrated rats [50]. The microarray analysis revealed that expression of a gene encoding the insulin-like factor 3 peptide (INSL3) and essential for testis positioning was directly inhibited by MEHP, confirming previous observations following the administration of DBP and/or DEHP to rats and mice in vivo [15], [17], [51] or the exposure of mouse and rat fetal Leydig cells [52].…”

Section: Discussionmentioning

confidence: 99%

Mono-(2-ethylhexyl) Phthalate Directly Alters the Expression of Leydig Cell Genes and CYP17 Lyase Activity in Cultured Rat Fetal Testis

Chauvigné

Plummer²,

Lesné

et al. 2011

PLoS ONE

View full text Add to dashboard Cite

Exposure to phthalates in utero alters fetal rat testis gene expression and testosterone production, but much remains to be done to understand the mechanisms underlying the direct action of phthalate within the fetal testis.We aimed to investigate the direct mechanisms of action of mono-(2-ethylhexyl) phthalate (MEHP) on the rat fetal testis, focusing on Leydig cell steroidogenesis in particular.We used an in vitro system based on the culture for three days, with or without MEHP, of rat fetal testes obtained at 14.5 days post-coitum.Exposure to MEHP led to a dose-dependent decrease in testosterone production. Moreover, the production of 5 alpha-dihydrotestosterone (5α-DHT) (−68%) and androstenedione (−54%) was also inhibited by 10 µM MEHP, whereas 17 alpha-hydroxyprogesterone (17α-OHP) production was found to increase (+41%). Testosterone synthesis was rescued by the addition of androstenedione but not by any of the other precursors used. Thus, the hormone data suggested that steroidogenesis was blocked at the level of the 17,20 lyase activity of the P450c17 enzyme (CYP17), converting 17α-OHP to androstenedione. The subsequent gene expression and protein levels supported this hypothesis. In addition to Cyp17a1, microarray analysis showed that several other genes important for testes development were affected by MEHP. These genes included those encoding insulin-like factor 3 (INSL3), which is involved in controlling testicular descent, and Inha, which encodes the alpha subunit of inhibin B.These findings indicate that under in vitro conditions known to support normal differentiation of the fetal rat testis, the exposure to MEHP directly inhibits several important Leydig cell factors involved in testis function and that the Cyp17a1 gene is a specific target to MEHP explaining the MEHP-induced suppression of steroidogenesis observed.

show abstract

Section: Discussionmentioning

confidence: 99%

Mono-(2-ethylhexyl) Phthalate Directly Alters the Expression of Leydig Cell Genes and CYP17 Lyase Activity in Cultured Rat Fetal Testis

Chauvigné

Plummer²,

Lesné

et al. 2011

PLoS ONE

View full text Add to dashboard Cite

show abstract

“…Several authors have reported that prostatic PBP levels notably decrease in the epithelium of castrated animals (Heyns et al, ; Aumuller et al, ; Janulis et al, ). Unpublished observations indicate that galectin‐1, another potent inmmunosuppresive factor, is positively regulated by androgens in the prostate gland.…”

Section: Androgen Modulation Of Host Defense Moleculesmentioning

confidence: 99%

“…Several authors have reported that prostatic PBP levels notably decrease in the epithelium of castrated animals (Heyns et al, 1978;Aumuller et al, 1982;Janulis et al, 2000).…”

Section: Androgen Modulation Of Host Defense Moleculesmentioning

confidence: 99%

Androgen regulation of host defenses and response to inflammatory stimuli in the prostate gland

Quintar

Maldonado

2017

Cell Biology International

View full text Add to dashboard Cite

The prostate gland is a strictly androgen-dependent organ which is also the main target of infectious and inflammatory diseases in the male reproductive tract. Host defenses and immunity of the gland have unique features to maintain a constant balance between response and tolerance to diverse antigens. In this context, the effects of reproductive hormones on the male tract are thus complex and have just started to be defined. From the classical description of "the prostatic antibacterial factor," many host defense proteins with potent microbicidal and anti-tumoral activities have been described in the organ. Indeed, it has been proposed a central role for resident cells, that is, epithelial and smooth muscle cells, in the prostatic response against injuries. However, these cells also represent the target of the inflammatory damage, leading to the development of a Proliferative Inflammatory Atrophy-like process in the epithelium and a myofibroblastic-like reactive stroma. Available data on androgen regulation of inflammation led to a model of the complex control, in which the final effect will depend on the tissue microenvironment, the cause of inflammation, and the levels of androgens among other factors. In this paper, we review the current scientific literature about the inflammatory process in the gland, the modulation of host defense proteins, and the influence of testosterone on the resolution of prostatitis.

show abstract

The Prostate Journal Database

2000

Prostate J

View full text Add to dashboard Cite

Prostatic luminal cell differentiation and prostatic steroid‐binding protein (PBP) gene expression are differentially affected by neonatal castration

Cited by 4 publications

References 41 publications

Mono-(2-ethylhexyl) Phthalate Directly Alters the Expression of Leydig Cell Genes and CYP17 Lyase Activity in Cultured Rat Fetal Testis

Mono-(2-ethylhexyl) Phthalate Directly Alters the Expression of Leydig Cell Genes and CYP17 Lyase Activity in Cultured Rat Fetal Testis

Androgen regulation of host defenses and response to inflammatory stimuli in the prostate gland

The Prostate Journal Database

Contact Info

Product

Resources

About