Prostatic acid phosphatase in the serially transplantable human prostatic tumor lines PC-82 and PC-EW

Csapo, Z.; Brand, Karl; Schrott, K. M.; Schwindl, B.

doi:10.1007/bf00302475

Cited by 3 publications

(3 citation statements)

References 11 publications

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“…Since none of the human prostate cultured cell lines or the Dunning rat prostate tumors express these antigens, we are left, at present, with determining if any of the human prostate heterotransplant lines produce the TURP-27, PD 41, and PSP antigens. Studies are in progress to evaluate whether the androgen-dependent PAP and PSA producing heterotransplant lines PC-82, PC-EW, or PC-EG [33,34] express these antigens.…”

Section: Discussionmentioning

confidence: 99%

Dunning rat prostate tumors and cultured cell lines fail to express human prostate carcinoma‐associated antigens

1990

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The objective of this study was to determine if human prostate carcinoma-associated tumor markers were expressed by Dunning rat prostate carcinomas. Frozen and formalin-fixed paraffin-embedded tissues from 12 different sublines of Dunning tumors were evaluated for marker expression by immunoperoxidase staining by using a panel of 9 monoclonal antibodies, including antibodies against human PAP and PSA. None of the Dunning tumors were found to express any of the human prostate tumor markers. Both fixed and live immunofluorescent assays were performed on 5 cultured Dunning tumor cell lines, evaluated either as single cells or as monolayers. As with the Dunning tumor tissues, none of the cell cultures expressed any of the 9 human prostate tumor markers. The lack of antigen expression by the Dunning tumor tissues and cell lines suggests that these human prostate tumor markers are quite species specific. These results limit the use of the Dunning prostate tumors as models to explore the preclinical application of these human prostate carcinoma-associated monoclonal antibodies and their target antigens.

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Section: Discussionmentioning

confidence: 99%

Dunning rat prostate tumors and cultured cell lines fail to express human prostate carcinoma‐associated antigens

1990

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“…At the histological level, in the PC‐82 heterotransplant, epithelial height decreases with focal desquamation, the acinar lumina widens (dilated glandular lumen), and the tumour shrinks and foci are cleared. At the cytological level, the cytoplasm is vacuolized and apparent partly vacuolized and pyknotic nuclei with less distinct nucleoli, compared to control heterotransplanted tumours in intact mice [21, 23, 66–68]. Moreover, the PC‐EW heterotransplant tumour responded more substantially to androgen deprivation.…”

Section: Serially Heterotransplanted Human Tumours In Immunosuppressementioning

confidence: 99%

Serially heterotransplanted human prostate tumours as an experimental model

López-Barcons

2010

J Cellular Molecular Medi

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Preclinical research on prostate cancer (PC) therapies uses several models to represent the human disease accurately. A common model uses patient prostate tumour biopsies to develop a cell line by serially passaging and subsequent implantation, in immunodeficient mice. An alternative model is direct implantation of patient prostate tumour biopsies into immunodeficient mice, followed by serial passage in vivo. The purpose of this review is to compile data from the more than 30 years of human PC serial heterotransplantation research. Serially heterotransplanted tumours are characterized by evaluating the histopathology of the resulting heterotransplants, including cellular differentiation, karyotype, marker expression, hormone sensitivity, cellular proliferation, metastatic potential and stromal and vascular components. These data are compared with the initial patient tumour specimen and, depending on available information, the patient’s clinical outcome was compared with the heterotransplanted tumour. The heterotansplant model is a more accurate preclinical model than older generation serially passaged or genetic models to investigate current and newly developed androgen-deprivation agents, antitumour compounds, anti-angiogenic drugs and positron emission tomography radiotracers, as well as new therapeutic regimens for the treatment of PC.

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“…All three xenograft lines retain the histopathologic characteristics of the patient's original tumor, are androgen dependent, and secrete the prostate tumor markers prostatic acid phosphatase (PAP) and prostate-specific antigen (PSA) [ 12,131. These prostate carcinoma heterotransplant lines represent the most frequently occurring types of human prostate cancer and have proven to be very useful in studying the in vivo effect of endocrine manipulation on tumor growth and on the expression and secretion of PAP and PSA [6,8,10,11].…”

Section: Introductionmentioning

confidence: 99%

Immunohistochemical evaluation of the expression of prostate tumor‐association markers in the nude mouse human prostate carcinoma heterotransplant lines PC‐82, PC‐EW, and PC‐EG

et al. 1990

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The biotin-avidin immunoperoxidase assay was used to evaluate the expression of several prostate carcinoma-associated markers in formalin-fixed paraffin-embedded tissue sections of three human prostate nude mouse heterotransplant lines PC-82, PC-EW, and PC-EG. In addition to monoclonal antibodies to PSA and PAP, monoclonal antibodies to five other potentially useful markers for prostate carcinomas (TURP-27, Leu-7, 7E11-C5, PSP-19, and PD41) were tested. Tissues from two or more transplant passages were evaluated. The human prostate target antigens were found to be expressed by one or more of the three heterotransplant lines. The PC-82 and PC-EW lines were the most efficient in terms of expression of multiple prostate carcinoma-associated markers and percentage of tumor cells positive for a given prostate antigen. The staining pattern of each marker, in terms of staining intensity, number of tumor cells stained, and staining location, i.e., membrane, cytoplasmic, or ductal secretions, was similar to what has been observed in tissue sections from human prostate carcinomas. The lack of an appropriate model for evaluating the preclinical potential of these Mabs (especially TURP-27, PSP-19, and PD41) makes the findings of this study of considerable importance, and suggests that these human prostate xenografts may be useful models for exploring the diagnostic and therapeutic potential of these anti-prostate carcinoma monoclonal antibodies.

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Prostatic acid phosphatase in the serially transplantable human prostatic tumor lines PC-82 and PC-EW

Cited by 3 publications

References 11 publications

Dunning rat prostate tumors and cultured cell lines fail to express human prostate carcinoma‐associated antigens

Dunning rat prostate tumors and cultured cell lines fail to express human prostate carcinoma‐associated antigens

Serially heterotransplanted human prostate tumours as an experimental model

Immunohistochemical evaluation of the expression of prostate tumor‐association markers in the nude mouse human prostate carcinoma heterotransplant lines PC‐82, PC‐EW, and PC‐EG

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