Prostatein or Steroid Binding Protein (PSBP) Induces Experimental Autoimmune Prostatitis (EAP) in NOD Mice

Rivero, Virginia; Carnaud, Claude; Riera, Clelia M.

doi:10.1006/clim.2002.5281

Cited by 49 publications

(55 citation statements)

References 29 publications

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“…Further experiments by the same group have shown that 1.0 mg Wistar rat prostate homogenate or 30 mg of Wistar rat PSBP injected twice resulted in the induction of prostatitis in 80-100% of young male NOD mice but in only B30% of C57BL/6 mice. 101 Although precise sites of prostate inflammation were not specified in this study, histologic changes in the prostate were characterized by perivascular and stromal MNC infiltration accompanied by edema. The edema was more severe in mice immunized with prostate homogenate than in those immunized by PSBP.…”

Section: Antigen-induced Mouse Prostatitis Models (Category Iii)mentioning

confidence: 88%

Experimental rodent models of prostatitis: limitations and potential

Vykhovanets

Resnick

MacLennan

et al. 2007

Prostate Cancer Prostatic Dis

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Prostatitis is a polyetiological inflammation of the prostate gland in men characterized by pelvic pain, irritative voiding symptoms, and sexual dysfunction. Histologically prostatitis is characterized by poly-and mononuclear cell infiltrates (neutrophils, lymphocytes, macrophages and plasma cells) in the stromal connective tissue around the acini or ducts. Prostatitis is an important worldwide health problem in men. The pathogenesis and diagnostic criteria for the condition are obscure, with the result that the development of management programs for this condition has been hindered. Animal model(s) might be useful in elucidating mechanisms involved in the molecular pathogenesis of chronic nonbacterial prostatitis and chronic pelvic pain syndrome. Given that prostatitis might have a multifactorial etiology, several animal models with unique features may prove helpful. This review examines a number of experimental rodent models of prostatitis and evaluates their advantages and limitations.

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Section: Antigen-induced Mouse Prostatitis Models (Category Iii)mentioning

confidence: 88%

Experimental rodent models of prostatitis: limitations and potential

Vykhovanets

Resnick

MacLennan

et al. 2007

Prostate Cancer Prostatic Dis

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“…This protocol induces EAP in 100% of male NOD mice, characterized by a florid leukocyte infiltrate into the prostate (23). Subsequent work has shown that EAP can also be induced in NOD mice by injection of prostatic steroid-binding protein (PSBP), also known as prostatein (24), a major autoantigen in rodent EAP (25,26). The male NOD mouse responds to PSBP by developing specific cellular and humoral autoimmune responses associated with inflammatory infiltrates in the prostate (24).…”

Section: Treatment Of Experimental Autoimmune Prostatitis In Nonobesementioning

confidence: 99%

“…Subsequent work has shown that EAP can also be induced in NOD mice by injection of prostatic steroid-binding protein (PSBP), also known as prostatein (24), a major autoantigen in rodent EAP (25,26). The male NOD mouse responds to PSBP by developing specific cellular and humoral autoimmune responses associated with inflammatory infiltrates in the prostate (24). In addition, using NOD mice lacking expression of ␤ 2 -microglobulin or MHC class II ␤-chain, an essential role for CD4 ϩ T cells has been shown in the development of EAP in this model (24).…”

Section: Treatment Of Experimental Autoimmune Prostatitis In Nonobesementioning

confidence: 99%

Treatment of Experimental Autoimmune Prostatitis in Nonobese Diabetic Mice by the Vitamin D Receptor Agonist Elocalcitol

Penna

Amuchastegui

Cossetti

et al. 2006

The Journal of Immunology

116

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On the basis of on the marked inhibitory activity of the vitamin D receptor agonist Elocalcitol on basal and growth factor-induced proliferation of human prostate cells and on its potent anti-inflammatory properties, we have tested its capacity to treat experimental autoimmune prostatitis (EAP) induced by injection of prostate homogenate-CFA in nonobese diabetic (NOD) mice. Administration of Elocalcitol, at normocalcemic doses, for 2 wk in already established EAP significantly inhibits the intraprostatic cell infiltrate, leading to a profound reduction in the number of CD4+ and CD8+ T cells, B cells, macrophages, dendritic cells, and I-Ag7-positive cells. Immunohistological analysis demonstrates reduced cell proliferation and increased apoptosis of resident and infiltrating cells. Significantly decreased production of the proinflammatory cytokines IFN-γ and IL-17 is observed in prostate-draining lymph node T cells from Elocalcitol-treated NOD mice stimulated by TCR ligation. In addition, Elocalcitol treatment reduces IFN-γ production by prostate-infiltrating CD4+ T cells and draining lymph node T cells specific for an immunodominant peptide naturally processed from prostate steroid-binding protein, a prostate-specific autoantigen. Finally, CD4+ splenic T cells from Elocalcitol-treated NOD mice show decreased ability, upon adoptive transfer into NOD.SCID recipients, to induce autoimmune prostatitis, paralleled by a reduced capacity to produce IFN-γ in response to prostate steroid-binding protein. The results indicate that Elocalcitol is able to interfere with key pathogenic events in already established EAP in the NOD mouse. These data show a novel indication for vitamin D receptor agonists and indicate that treatment with Elocalcitol may inhibit the intraprostatic inflammatory response in chronic prostatitis/chronic pelvic pain syndrome patients.

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“…To further elucidate the pathogenesis of human CPPS, a few rodent models of experimental autoimmune prostatitis have been developed and characterized (10). Immunization of rats or mice with prostate extracts or proteins expressed in the prostate, such as prostatic acid phosphatase, induces specific immune responses to prostate antigens and, in some models, induces prostatitis characterized by lymphocyte infiltration (11)(12)(13). Prostatitis can also be induced in mice with thymectomy on day 3 of life (14).…”

Section: Introductionmentioning

confidence: 99%

An aberrant prostate antigen–specific immune response causes prostatitis in mice and is associated with chronic prostatitis in humans

Hou¹,

DeVoss²,

Dao³

et al. 2009

J. Clin. Invest.

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Chronic prostatitis is a common disease of unclear etiology and has no specific treatment. Mice deficient in the expression of the autoimmune regulator (Aire) gene, which are defective in thymic expression of self antigens and central tolerance, develop spontaneous prostatitis. In this study, we found that Aire-deficient mice developed spontaneous B and T cell immune responses to a prostate autoantigen, seminal vesicle secretory protein 2 (SVS2), which we believe to be novel. We show that thymic expression of this self antigen was Aire dependent. Moreover, prostatitis was induced in WT mice through immunization with SVS2, demonstrating that immunity to SVS2 was sufficient to induce prostatitis. The clinical relevance of this antigen was highlighted by our observation that patients with chronic prostatitis possessed specific autoantibodies against the human SVS2-like seminal vesicle protein semenogelin. These results provide direct evidence that spontaneous chronic prostatitis is an autoimmune disease and is regulated by both central and peripheral tolerance. Moreover, SVS2 and semenogelin are among the relevant autoantigens in mice and humans, respectively.

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Prostatein or Steroid Binding Protein (PSBP) Induces Experimental Autoimmune Prostatitis (EAP) in NOD Mice

Cited by 49 publications

References 29 publications

Experimental rodent models of prostatitis: limitations and potential

Experimental rodent models of prostatitis: limitations and potential

Treatment of Experimental Autoimmune Prostatitis in Nonobese Diabetic Mice by the Vitamin D Receptor Agonist Elocalcitol

An aberrant prostate antigen–specific immune response causes prostatitis in mice and is associated with chronic prostatitis in humans

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