Prostate Specific Antigen. Current clinical application and future prospects

Duskova, Katerina; Veselý, Štěpán

doi:10.5507/bp.2014.046

Cited by 16 publications

(13 citation statements)

References 97 publications

(77 reference statements)

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“…Інгібітори протеази в сироватці, такі як α1-антихімотрипсин (α1-ACT), α2-макроглобулін (α2-M) та інші білки гострого стану, утворюють незворотні комплекси з циркулюючим PSA і гальмують його протеолітичну активність. У плазмі сперми й, відповідно, у сироватці (плазмі) крові PSA може перебувати у вільній інтактній (неактивній) протеолітичній формі або бути зв'язаним з інгібіторами серинової протеазної активності [1][2][3][4]. Більша частина PSA (близько 75 %), потрапляючи в периферійний кровотік, стає неактивною через утворення комплексу молекулярною масою 90 кДа з α1-ACT.…”

Section: вступunclassified

“…Визначення меж виявлення та кількісного визначення (аналітичної чутливості) аналізу. Розрахункове визначення цих показників проводили через попереднє визначення стандартного відхилення для нульової концентрації Sx за формулою (2). Межу виявлення розраховували як Sx  3,3, а аналітичну чутливістьяк Sx  10, спираючись на відповідні рекомендації [11,15].…”

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Development and Validation of a Highly Informative Immuno-Enzymatic Analysis for the Determination of Free Prostat-Specific Antigen

Komar¹,

Kozerecka²,

Besarab³

et al. 2019

Innov Biosyst Bioeng

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Section: вступunclassified

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Development and Validation of a Highly Informative Immuno-Enzymatic Analysis for the Determination of Free Prostat-Specific Antigen

Komar¹,

Kozerecka²,

Besarab³

et al. 2019

Innov Biosyst Bioeng

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“…PSA is a biomarker for PC risk assessment. Serum protease inhibitors such as α1-antichymotrypsin (α 1 -ACT), α 2 -macroglobulin (α 2 -MG), and other acute-doi: https://doi.org/10.15407/ubj91.01.030 protein proteins form irreversible complexes with circulating PSA and inhibit its proteolytic activity [1][2][3][4][5][6][7].…”

mentioning

confidence: 99%

Different mice inbred strains humoral immune response against human prostate-specific antigen

Galkin¹,

Komar²,

Besarab³

2019

Ukr.Biochem.J.

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The aim of the study was an investigation of humoral immune response to prostate-specific antigen (PSA) of different mice inbred strains for further development of recommendations for appropriate immunization schemes for monoclonal antibodies (mcabs) obtaining. The study was conducted using: Balb/c and NZB mice; PSA from human sperm (as immunogen). In case of booster immunization immunogen was previously diluted to the desired concentration (10 or 30 µg per 100 µl) in saline, and injected in the tail vein or intraperitoneally. In case of other immunizations, we prepared emulsion solution of immunogen with adjuvant to final concentration of 10 or 30 µg per 100 µl: PSa was dissolved in saline, the same volume of adjuvant was added, and mixture was thoroughly mixed to form a stable emulsion. When subcutaneous administration, total dose of 100 µl was divided into two equal parts and injected in the hind paw of mice. Intraperitoneal immunization was performed by single administration of 100 µl of emulsion. The level of specific antibodies was determined by titration of blood sera from animals in indirect elISa. Series of experiments were conducted to determine the level of humoral response of mice of different inbred strains (Balb/c and NZB) for multi-stage immunization with different duration with different amounts of PSA (10 and 30 μg), with different immunoglobulin administration (intraperitoneal and subcutaneously), and with different adjuvants (Freund's complete and incomplete adjuvants, FCA/FIA). Final booster immunization at a dose of 30 μg was performed either in the same manner as the previous administration of the immunogen, or intravenously in the physio logical saline solution. Dependencies of the humoral immune response of Balb/c and NZB mice against PSa on the route of administration of immunogen, the dose and duration of immunization were established. It was shown that intraperitoneal administration provided formation of higher titers of specific antibodies in case of both mice strains. Balb/c mice lines more rapidly responded to PSa, than an NZB mice (for all investigated schemes immunization). It was shown that the most effective immunization scheme was three times intraperitoneal administration with 10 µg of PSA for 8 weeks (the first immunization with FCA, and the rest-with FIA) and booster immunogen intravenous administration in saline solution.

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“…Although early detection of prostate cancer is crucial, several biomarkers for early screening are still controversial [4,5]. Over 70% of the patients are cured after various primary treatment options such as surgery, radiotherapy and androgen-deprivation therapy [2,6].…”

Section: Introductionmentioning

confidence: 99%

Doxycycline down-regulates matrix metalloproteinase expression and inhibits NF-κB signaling in LPS-induced PC3 cells

Ogut

Reel

Korkmaz

et al. 2017

Folia Histochem Cytobiol.

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Introduction. Matrix metalloproteinase enzymes (MMPs) play important role in inflammation, malignant cell proliferation, invasion and angiogenesis by mediating extracellular matrix degradation. Doxycycline, a synthetic tetracycline, behaves as a MMP inhibitor at a subantimicrobial dose and inhibits tumor cell proliferation, invasion and angiogenesis. The aberrant activity of nuclear factor kappa B (NF-kB) causes activation of MMPs and thereby proliferation and invasion of cancer cells. The aim of this study was to investigate the effects of doxycycline on the expression of MMPs in lipopolysaccharide (LPS)-induced PC3 human prostate cancer cells and the possible role of NF-kB signaling. Material and methods. PC3 cells were incubated with LPS (0.5 µg/mL) for 24 h in the presence or absence of doxycycline (5 µg/mL). The effects of LPS and doxycycline on the expressions of MMP-2, MMP-8, MMP-9, MMP-10, NF-kB/p65, IkB-a, p-IkB-a, IKK-b were examined by Western blotting and immunohistochemistry in PC3 cells. Furthermore, relative proteinase activities of MMP-2 and MMP-9 were determined by gelatin zymography. Results. LPS increased expression and activity of MMP-9 and expression of MMP-8, MMP-10, NF-kB/p65, p-IkB-a, IKK-b and doxycycline down-regulated its effects with the exception of MMP-10 expression. The expression of MMP-2 and IkB-a was affected by neither LPS nor doxycycline. Conclusions. Our findings indicate that doxycycline inhibits the expression of various MMPs and NF-kB signaling may play a role in the regulation of MMPs expression in LPS-induced PC3 human prostate cancer cells.

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Prostate Specific Antigen. Current clinical application and future prospects

Cited by 16 publications

References 97 publications

Development and Validation of a Highly Informative Immuno-Enzymatic Analysis for the Determination of Free Prostat-Specific Antigen

Development and Validation of a Highly Informative Immuno-Enzymatic Analysis for the Determination of Free Prostat-Specific Antigen

Different mice inbred strains humoral immune response against human prostate-specific antigen

Doxycycline down-regulates matrix metalloproteinase expression and inhibits NF-κB signaling in LPS-induced PC3 cells

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