Prostanoid TP receptor stimulation enhances contractile activities in guinea pig urinary bladder smooth muscle through activation of Ca2+ entry channels: Potential targets in the treatment of urinary bladder contractile dysfunction

Ou, Guanghan; Fujisawa, Miki; Yashiro, Ayano; Xu, Keyue; Yoshioka, Kento; Obara, Kazuo; Tanaka, Yoshio

doi:10.1016/j.lfs.2021.120130

Cited by 5 publications

(3 citation statements)

References 25 publications

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“…To date, many pharmacological studies have used SKF-96365 as a selective inhibitor for SOCC to identify this Ca 2+ influx route in chemically stimulated responses. 16,[23][24][25][26][27] We also showed that a CPA-induced increase in [Ca 2+ ] i within 293T cells, which was insensitive to LOE 908, was strongly inhibited by SKF-96365, suggesting that this inhibitor is a useful pharmacological tool for SOCC. However, SKF-96365 also inhibits VDCC/ROCC in addition to SOCC.…”

Section: Discussionmentioning

confidence: 63%

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Pharmacological Studies on the Ca2+ Influx Pathways in Platelet-Activating Factor (PAF)-Induced Mouse Urinary Bladder Smooth Muscle Contraction

Liu

Obara

Yoshioka

et al. 2023

Biological & Pharmaceutical Bulletin

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Platelet-activating factor (PAF) not only acts as a mediator of platelet aggregation, inflammation, and allergy responses but also as a constrictor of various smooth muscle (SM) tissues, including gastrointestinal, tracheal/bronchial, and pregnancy uterine SMs. Previously, we reported that PAF induces basal tension increase (BTI) and oscillatory contraction (OC) in mouse urinary bladder SM (UBSM). In this study, we examined the Ca 2 influx pathways involved in PAF-induced BTI and OC in the mouse UBSM. PAF (10 6 M) induced BTI and OC in mouse UBSM. However, the PAF-induced BTI and OC were completely suppressed by extracellular Ca 2 removal. PAF-induced BTI and OC frequencies were markedly suppressed by voltagedependent Ca 2 channel (VDCC) inhibitors (verapamil (10 5 M), diltiazem (10 5 M), and nifedipine (10 7 M)). However, these VDCC inhibitors had a minor effect on the PAF-induced OC amplitude. The PAF-induced OC amplitude in the presence of verapamil (10 5 M) was strongly suppressed by SKF-96365 (3 10 5 M), an inhibitor of receptor-operated Ca 2 channel (ROCC) and store-operated Ca 2 channel (SOCC), but not by LOE-908 (3 10 5 M) (an inhibitor of ROCC). Overall, PAF-induced BTI and OC in mouse UBSM depend on Ca 2 influx and the main Ca 2 influx pathways in PAF-induced BTI and OC may be VDCC and SOCC. Of note, VDCC may be involved in PAF-induced BTI and OC frequency, and SOCC might be involved in PAF-induced OC amplitude. Key words platelet-activating factor (PAF), mouse urinary bladder smooth muscle, Ca 2+ influx pathway, voltage-dependent Ca 2+ channel (VDCC), store-operated Ca 2+ channel (SOCC), receptor-operated Ca 2+ channel (ROCC)

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Section: Discussionmentioning

confidence: 63%

“…Data Analysis PAF-induced BTI and the amplitude/ frequency of PAF-induced OC were analyzed as previously reported. 12,16) Briefly, BTI/OC were calculated over 3 min using the LabChart™ (Version 7) software (ADInstruments Pty. Ltd., Bella Vista, NSW, Australia): immediately before…”

Section: Methodsmentioning

confidence: 99%

Pharmacological Studies on the Ca2+ Influx Pathways in Platelet-Activating Factor (PAF)-Induced Mouse Urinary Bladder Smooth Muscle Contraction

Liu

Obara

Yoshioka

et al. 2023

Biological & Pharmaceutical Bulletin

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“…RT-qPCR was performed according to the methods described in a previous report. 76) Briefly, total RNA was extracted from the endothelium-denuded TA preparations by the acid guanidinium thiocyanate-phenol-chloroform method. After treated with deoxyribonuclease (Nippon Gene Co., Ltd., Tokyo, Japan) for 30 min at 37 °C, total RNA pellets were obtained by phenol-chloroform extraction/ethanol precipitation and dissolved in diethyl pyrocarbonate-treated water.…”

Section: Measurement Of Tension Changesmentioning

confidence: 99%

Phenylephrine-Induced Contraction in Guinea Pig Thoracic Aorta Is Triggered by Stimulation of α1L-Adrenoceptors Functionally Coupled with Store-Operated Ca2+ Channels and Voltage-Dependent Ca2+ Channels

Obara

Yoshioka

Regadera

et al. 2023

Biological & Pharmaceutical Bulletin

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We examined whether the α 1L -adrenoceptor (AR), which shows low affinity (pA 2 < 9) for prazosin (an α 1 -AR antagonist) and high affinity (pA 2 ≈ 10) for tamsulosin/silodosin (α 1A -AR antagonists), is involved in phenylephrine-induced contractions in the guinea pig (GP) thoracic aorta (TA). Intracellular signaling induced by α 1L -AR activation was also examined by focusing on Ca 2 influx pathways. Tension changes of endothelium-denuded TAs were isometrically recorded and mRNA encoding α-ARs/Ca 2 channels and their related molecules were measured using RT-quantitative PCR. Phenylephrine-induced contractions were competitively inhibited by prazosin/tamsulosin, and their pA 2 value were calculated to be 8.53/9.74, respectively. These contractions were also inhibited by silodosin concentration-dependently. However, the inhibition was not competitive fashion with the apparent pA 2 value being 9.48. In contrast, phenylephrine-induced contractions were not substantially suppressed by L-765314 (an α 1B -AR antagonist), BMY 7378 (an α 1D -AR antagonist), yohimbine, and idazoxan (α 2 -AR antagonists). Phenylephrine-induced contractions were markedly inhibited by YM-254890 (a Gq protein inhibitor) or removal of extracellular Ca 2 , and partially inhibited by verapamil (a voltage-dependent Ca 2 channel (VDCC) inhibitor). The residual contractions in the presence of verapamil were slightly inhibited by LOE 908 (a receptor-operated Ca 2 channel (ROCC) inhibitor) and strongly inhibited by SKF-96365 (a store-operated Ca 2 channel (SOCC) and ROCC inhibitor). Among the mRNA encoding α-ARs/SOCC-related molecules, α 1A -AR (Adra1a)/Orai3, Orai1, and Stim2 were abundant in this tissue. In conclusion, phenylephrine-induced contractions in the GP TA can be triggered by stimulation of Gq protein-coupled α 1L -AR, followed by activation of SOCCs and VDCCs.

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Platelet-activating factor (PAF) enhances guinea pig detrusor smooth muscle contractile activities by stimulating voltage-dependent Ca2+ channels and store-operated Ca2+ channels

Obara

Kaneko

Yamashita

et al. 2023

Journal of Pharmacological Sciences

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Prostanoid TP receptor stimulation enhances contractile activities in guinea pig urinary bladder smooth muscle through activation of Ca2+ entry channels: Potential targets in the treatment of urinary bladder contractile dysfunction

Cited by 5 publications

References 25 publications

Pharmacological Studies on the Ca<sup>2+</sup> Influx Pathways in Platelet-Activating Factor (PAF)-Induced Mouse Urinary Bladder Smooth Muscle Contraction

Pharmacological Studies on the Ca<sup>2+</sup> Influx Pathways in Platelet-Activating Factor (PAF)-Induced Mouse Urinary Bladder Smooth Muscle Contraction

Phenylephrine-Induced Contraction in Guinea Pig Thoracic Aorta Is Triggered by Stimulation of α<sub>1L</sub>-Adrenoceptors Functionally Coupled with Store-Operated Ca<sup>2+</sup> Channels and Voltage-Dependent Ca<sup>2+</sup> Channels

Platelet-activating factor (PAF) enhances guinea pig detrusor smooth muscle contractile activities by stimulating voltage-dependent Ca2+ channels and store-operated Ca2+ channels

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