Prostaglandins of the A Series Inhibit Sendai Virus Replication in Cultured Cells

Abstract

SUMMARYProstaglandins of the A series (PGA) were shown to be potent inhibitors of Sendai virus replication in African green monkey kidney cells in culture. This antiviral activity was specific for PGA. The effective dose (4 pg/ml) was not toxic to the cells and did not alter either host cell metabolism or infectivity of the virus. PGA did not affect the first stages of virus replication and seemed to act by inhibiting virus maturation and/or budding from the cells. The antiviral action of PGA was pharmacological, was not mediated by cAMP and was completely reversible. Possible mechanisms of action include post-translational binding to virus proteins or interaction with interferon.

Antiviral Activity of Prostaglandin A on Encephalomyocarditis Virus-infected Cells: A Unique Effect Unrelated to Interferon

“…Our results confirm and extend previous reports of antiviral effects of prostaglandins of the A series (Santoro et al, 1980(Santoro et al, , 1981(Santoro et al, , 1982a(Santoro et al, , 1983a, adding EMC virus to the list of inhibitable viruses. The effect involves neither infection nor virus release, since P G A added during 1 to 3 h or during the last 2 h after infection was without consequence for the yield of new virus.…”

“…There was no difference in antiviral potency when PGAs were added together with the infecting virus as compared to adding them 1 h later (data not shown). In agreement with the results with Sendai virus (Santoro et al, 1981) no antiviral effect was seen when the cells were preincubated with PGA~ for 5 h, then washed prior to infection and kept in the absence of PGA~ for the remainder of the incubation period. Also, the antiviral effect was identical when PGAt was either added to cells together with infecting virus or 5 h prior to infection, but then kept with the infected cells for the entire incubation period.…”

“…1, PGA1 and PGA2 were equally inhibitory to the propagation of EMC virus. Over 90~ inhibition of virus yield was observed at concentrations of 4 btg/ml, similar to inhibitory concentrations seen with other viruses (Santoro et al, 1980(Santoro et al, , 1981(Santoro et al, , 1982a(Santoro et al, , 1983a. There was no difference in antiviral potency when PGAs were added together with the infecting virus as compared to adding them 1 h later (data not shown).…”

“…Prostaglandins of the A series (PGA~, PGA2) have been shown to inhibit replication of RNA and DNA viruses such as Sendai, vesicular stomatitis and vaccinia viruses (Santoro et al, 1980(Santoro et al, , 1981(Santoro et al, , 1982(Santoro et al, a, 1983. The following observations have suggested a link between prostaglandin and interferon action.…”

Inhibition by Glucocorticosteroid Hormones of Interferon and Prostaglandin E Induction by Poly(rI).Poly(rC)

“…Thus, prostaglandin A has an antiviral action similar to IFNs (Santoro et al, 1980(Santoro et al, , 1981. Moreover, various PGs have anti-cellular action and inhibit turnout cell division as has been reported also for IFN (Honn et al, 1981 ;Gresser & Tovey, 1978).…”