Prostaglandin protects against bile salt induced increases in proton permeation of duodenal brush border membrane.

Zhao, Delai; Hirst, Barry H.

doi:10.1136/gut.32.6.645

Cited by 6 publications

(4 citation statements)

References 23 publications

(5 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…16 17 In vitro studies have shown that PGE2 exerts a direct protective action on duodenal luminal cell membrane vesicles incubated with deoxycholate. '7 These findings suggest that the mucosal eicosanoid response to intraluminal irritants is a protective response.…”

Section: Discussionmentioning

confidence: 99%

Bile acid induced colonic irritation stimulates intracolonic nitric oxide release in humans.

Casellas

Mourelle

Papo

et al. 1996

Gut

View full text Add to dashboard Cite

It has been shown that bile acids increase the production of reactive oxygen in colonic mucosal scrapings and isolated colonic crypts.7The degradation substances produced by the peroxidative process initiated by oxygen radicals can diffuse away causing oedema, increasing vascular permeability and inflammation.8 Nitric oxide (NO) may contribute to the actions of oxygen superoxide anion radical by forming peroxynitrite, which is decomposed into the potent oxidants OH and NO2.9 NO mediates many biological actions, such as smooth muscle relaxation, neurotransmission, inflammation or cytotoxicity.'°0 l However, the possible role of NO in the colonic mucosal response to bile acid induced irritation has not been previously explored.To investigate whether stimulation of NO synthesis participates in the response of colonic mucosa to bile acid irritation, we measured by a perfusion technique in humans the intracolonic release of NO derived products before and after colonic infusion of DCA in a group of patients with irritable bowel syndrome. Methods

show abstract

Section: Discussionmentioning

confidence: 99%

Bile acid induced colonic irritation stimulates intracolonic nitric oxide release in humans.

Casellas

Mourelle

Papo

et al. 1996

Gut

View full text Add to dashboard Cite

show abstract

“…In Necturus antrum, taurocholate increased apical membrane conductance associated with an intracellular acidification (Kiviluoto et al 1989). Similarly, in isolated rabbit duodenal brush-border membrane vesicles bile salts increased proton permeability, and this increase was ameliorated by PGE2 (Zhao & Hirst, 1991). Thus, one mechanism for the protective action of prostaglandins is the reduction in apical membrane proton permeability, through pathways yet to be defined.…”

Section: Discussionmentioning

confidence: 96%

Transmucosal electrical resistance in rabbit isolated gastric mucosa during exposure to acid.

Spencer

Spraggs

Stables

et al. 1992

The Journal of Physiology

Self Cite

View full text Add to dashboard Cite

SUMMARY1. Transmucosal electrical resistance (Rj) and short-circuit current (1S,) were determined in rabbit isolated fundic mucosa. Under basal conditions, with a HCO3--free HEPES-buffered solution (pH 7 4) bathing both sides of the mucosae, Rt was 161-5 + 5-0 Q cm2 and I_C 41 8 + 1P8 ,tA cm-2, and these values were not significantly different to values observed in HC03--buffered Krebs-Hensleit solution.2. The basal 'SC was inhibited by the Cl-channel blocker diphenylamine-2-carboxylate, and ouabain, but unaffected by the Na+ channel blocker amiloride (10-M), consistent with electrogenic chloride secretion dependent upon a sodium gradient. Prostaglandin E2 (10-7 M) stimulated an increase in ISC which was susceptible to inhibition by diphenylamine-2-carboxylate, but not amiloride, again consistent with Cl-secretion.3. Stepwise acidification of the mucosal solution to pH 2-8 resulted in an increase in Rt of 43%, as compared with that measured with mucosal pH 7-4. IC did not change during the acidification to pH 2-8, indicating retention of tissue viability. Increased Rt while IS remained constant is consistent with an acid-induced decrease in the shunt (paracellular) conductance in this Cl--secreting tissue. At pH < 2-8, Rt declined rapidly and ISC declined and reversed, consistent with H' back-diffusion.

show abstract

“…Bile acids, being amphiphilic compounds, are also able to directly interact with the lipid component of membranes. Interacting with membranes, bile acids can significantly change their structure and functions (detergent action) [ 14 , 15 , 16 , 17 ], as well as induce transmembrane transport of protons (protonophore action) [ 18 , 19 ] and divalent ions (ionophore action) [ 20 , 21 , 22 ]. At the same time, further studies are needed to elucidate the mechanisms of the protonophore and ionophore effects of bile acids in the mitochondria of animals and humans.…”

Section: Discussionmentioning

confidence: 99%

“…It is of note that hydrophobic bile acids are most effective in this case [ 14 , 15 , 16 , 17 ]. The protonophore [ 18 , 19 ] and ionophore [ 20 , 21 , 22 ] effects of bile acids are also known (see the next section for more details).…”

Section: Introductionmentioning

confidence: 99%

Bile Acids as Inducers of Protonophore and Ionophore Permeability of Biological and Artificial Membranes

et al. 2023

View full text Add to dashboard Cite

It is now generally accepted that the role of bile acids in the organism is not limited to their participation in the process of food digestion. Indeed, bile acids are signaling molecules and being amphiphilic compounds, are also capable of modifying the properties of cell membranes and their organelles. This review is devoted to the analysis of data on the interaction of bile acids with biological and artificial membranes, in particular, their protonophore and ionophore effects. The effects of bile acids were analyzed depending on their physicochemical properties: namely the structure of their molecules, indicators of the hydrophobic–hydrophilic balance, and the critical micelle concentration. Particular attention is paid to the interaction of bile acids with the powerhouse of cells, the mitochondria. It is of note that bile acids, in addition to their protonophore and ionophore actions, can also induce Ca2+-dependent nonspecific permeability of the inner mitochondrial membrane. We consider the unique action of ursodeoxycholic acid as an inducer of potassium conductivity of the inner mitochondrial membrane. We also discuss a possible relationship between this K+ ionophore action of ursodeoxycholic acid and its therapeutic effects.

show abstract

Prostaglandin protects against bile salt induced increases in proton permeation of duodenal brush border membrane.

Cited by 6 publications

References 23 publications

Bile acid induced colonic irritation stimulates intracolonic nitric oxide release in humans.

Bile acid induced colonic irritation stimulates intracolonic nitric oxide release in humans.

Transmucosal electrical resistance in rabbit isolated gastric mucosa during exposure to acid.

Bile Acids as Inducers of Protonophore and Ionophore Permeability of Biological and Artificial Membranes

Contact Info

Product

Resources

About