Prostaglandin F2α promotes angiogenesis and embryo–maternal interactions during implantation

Kaczyński, Piotr; Kowalewski, Mariusz P.; Wacławik, Agnieszka

doi:10.1530/rep-15-0496

Cited by 55 publications

(56 citation statements)

References 66 publications

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“…The effects of E2 administered directly into the uterine lumen was studied using in vivo models [88]. Prepubertal crossbred gilts of similar age (6 months) and genetic background after the second natural estrous cycle were treated hormonally with 750 I.U.…”

Section: Effect Of E2 On Porcine Endometrium In Vivomentioning

confidence: 99%

Estradiol-17β-Induced Changes in the Porcine Endometrial Transcriptome In Vivo

Kaczyński

Bauersachs

Baryla

et al. 2020

IJMS

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Estradiol-17β (E2) is a key hormone regulating reproductive functions in females. In pigs, E2, as the main conceptus signal, initiates processes resulting in prolonged corpus luteum function, embryo development, and implantation. During early pregnancy the endometrium undergoes morphological and physiological transitions that are tightly related to transcriptome changes. Recently, however, the importance of E2 as a primary conceptus signal in the pig has been questionable. Thus, the aim of the present study was to determine the effects of E2 on the porcine endometrial transcriptome in vivo and to compare these effects with transcriptome profiles on day 12 of pregnancy. Microarray analysis revealed differentially expressed genes (DEGs) in response to E2 with overrepresented functional terms related to secretive functions, extracellular vesicles, cell adhesion, proliferation and differentiation, tissue rearrangements, immune response, lipid metabolism, and many others. Numerous common DEGs and processes for the endometrium on day 12 of pregnancy and E2-treated endometrium were identified. In summary, the present study is the first evidence for the effect of E2 on transcriptome profiles in porcine endometrium in vivo in the period corresponding to the maternal recognition of pregnancy. The presented results provide a valuable resource for further targeted studies considering genes and pathways regulated by conceptus-derived estrogens and their role in pregnancy establishment.During early pregnancy in the pig, porcine conceptuses (embryos with associated membranes) secrete E2, which is regarded as the pregnancy recognition signal required for prolonged progesterone synthesis and secretion by corpora lutea (CLs). Porcine conceptuses secrete estrogens in a biphasic manner-the first increase of conceptus-derived estrogens occurs on days 11-13 after fertilization and the second is between days 15 and 25-30 after fertilization (reviewed in [2]). However, the levels of conceptus-derived estrogens in the uterine lumen may also vary depending on the number of conceptuses [1]. The period of elevated E2 synthesis and secretion by porcine conceptuses between days 11-13 of pregnancy is a process defined as the maternal recognition of pregnancy [5]. This is the critical period for establishment and development of pregnancy as the highest mortality rate of embryos in animals including pigs is observed during the peri-implantation period [6]. Embryonic estrogen synthesis and secretion not only prolongs the CL lifespan but also enhances P4-induced endometrial receptivity for implantation. Following the decrease of the progesterone receptor expression in the endometrial luminal and glandular epithelia, the expression of the estrogen receptor (ESR1) is up-regulated in these structures, which in turn is important for the cell-specific responses to conceptus estrogens released on day 12 of pregnancy [7]. Estrogen is involved in stimulation of uterine secretory activity [8], increased blood flow [9], endometrial edema [10], and the regul...

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Section: Effect Of E2 On Porcine Endometrium In Vivomentioning

confidence: 99%

Estradiol-17β-Induced Changes in the Porcine Endometrial Transcriptome In Vivo

Kaczyński

Bauersachs

Baryla

et al. 2020

IJMS

Self Cite

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“…However, it should not be regarded as undesirable factor in pregnancy because elevated amounts of PGF2α in the uterine lumen and/or its increased endometrial synthesis occur not only in pigs (Zavy et al 1980, Waclawik et al 2006 but also in other mammals including rodents, cattle, dogs and humans (Kennedy et al 2007, Ulbrich et al 2009, Wang et al 2010, Vilella et al 2013, Kowalewski et al 2014. Moreover, a novel role for PGF2α in the porcine endometrium during the peri-implantation period has been recently reported by our group, indicating that PGF2α participates in pregnancy establishment by promoting angiogenesis and expression of genes involved in endometrial remodeling and embryomaternal communication (Kaczyński et al 2016).…”

Section: Introductionmentioning

confidence: 73%

“…PGE2 has been described as playing a luteoprotective/luteotrophic role (Waclawik et al 2008, Waclawik 2011 whereas PGF2α has been mainly perceived as a luteolytic factor in sheep, cattle and pigs, as well as in rats, rabbit and hamsters (reviewed in McCracken et al 1999). Thus, it could also have a detrimental effect in pregnancy development in mammals, including human, cattle, pigs and dogs (reviewed in Kaczyński et al 2016). However, it should not be regarded as undesirable factor in pregnancy because elevated amounts of PGF2α in the uterine lumen and/or its increased endometrial synthesis occur not only in pigs (Zavy et al 1980, Waclawik et al 2006 but also in other mammals including rodents, cattle, dogs and humans (Kennedy et al 2007, Ulbrich et al 2009, Wang et al 2010, Vilella et al 2013, Kowalewski et al 2014.…”

Section: Introductionmentioning

confidence: 99%

“…Previously we indicated that synthesis of PGF2α during the implantation period is elevated both in the porcine endometrium and the conceptus (Waclawik et al 2006, Waclawik & Ziecik 2007. Recently, we also elucidated the mechanism of PGF2α action in the endometrium , Kaczyński et al 2016. Hence, the question arose whether PGF2α can act through its receptor in the conceptus and if so, what could be the role of PGF2α in porcine trophoblast cells?…”

Section: Introductionmentioning

confidence: 99%

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Prostaglandin F2α promotes embryo implantation and development in the pig

et al. 2018

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Successful establishment and development of pregnancy requires proper communication between developing conceptuses and the maternal reproductive tract. Prostaglandins are key players involved in the regulation of reproductive processes in mammals including pigs. Due to its luteolytic action, prostaglandin F2-alpha (PGF2α) is mainly considered as an undesirable factor during early pregnancy. However, its content in the uterine lumen is elevated in pigs and other mammals. Recently, we reported an important role of PGF2α in the endometrium during early pregnancy in the pig. Thus, the aim of the present study was to determine whether PGF2α can act on porcine trophoblast and if so, to elucidate what effect it could exert. We detected increased expression of PGF2α receptor during the implantation period (from day 14 until day 19 of pregnancy). Global gene expression profiling using microarrays and quantitative PCR studies revealed that PGF2α acting on porcine trophoblast cells in vitro alters expression of genes potentially involved in processes related to implantation, such as: cell proliferation, focal adhesion, extracellular matrix binding, cell migration, cytoskeleton organization, immune interactions, ion homeostasis, and lipid metabolism. Using primary porcine trophoblast cells, we demonstrated that PGF2α stimulated trophoblast cell proliferation and adhesion to extracellular matrix protein. This was likely mediated by mitogen-activated protein kinases (MAPK1/3) and focal adhesion kinase (FAK) since we observed increased phosphorylation of MAPK1/3 and FAK in trophoblast cells treated with PGF2α. To conclude, the present report indicates a novel role for PGF2α in the porcine conceptus as a para- and autocrine factor supporting pregnancy establishment.

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“…Alternatively, SGK1 contributes to early angiogenesis by inducing expression of Notch‐signaling genes (93). Although these findings have been conducted mainly on embryos and endothelial cell cultures, considering the highly conserved and ubiquitous expression of SGK1 , further studies in these areas might reveal a potential role for SGK1 in fertility and pregnancy maintenance from the perspective of vasculogenesis, which is a unique part of implantation (94). Of note, it has been suggested that SGK1 impairs human endometrial receptivity in diabetes (20).…”

Section: Discussionmentioning

confidence: 99%

Involvement of serum glucocorticoid‐regulated kinase 1 in reproductive success

Lou

Mao

et al. 2016

FASEB j.

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Reproductive processes, in particular events that concern pregnancy, are fine-tuned to produce offspring. Reproductive success is of prime importance for the survival of every species. The highly conserved and ubiquitously expressed serum glucocorticoid-regulated kinase 1 (SGK1) was first implicated in infertility as a regulator of a Na channel. In this review, we emphasize the prominent role of SGK1 during early pregnancy: 1) balancing uterine luminal fluid secretion and reabsorption to aid blastocyst adhesion and to import nutrients and energy; 2) transducing signals from the blastocyst to the receptive endometrium; 3) inducing multiple genes that are involved in uterine receptivity and trophoblast invasion; 4) regulating cell differentiation and antioxidant defenses at the fetomaternal interface; and 5) contributing to the proliferation and survival of decidual stromal cells. Accordingly, SGK1 coordinates many cellular processes that are crucial to reproductive activities. Aberrant expression or function of SGK1 results in implantation failure and early pregnancy loss. Further investigation of the molecular mechanisms of the function of SGK1 might provide novel diagnostic tools and interventions for reproductive complications.-Lou, Y., Hu, M., Mao, L., Zheng, Y., Jin, F. Involvement of serum glucocorticoid-regulated kinase 1 in reproductive success.

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Prostaglandin F2α promotes angiogenesis and embryo–maternal interactions during implantation

Abstract: Implantation in humans and other mammals is a critical period during which high embryonic mortality rates occur. Prostaglandins

Cited by 55 publications

References 66 publications

Estradiol-17β-Induced Changes in the Porcine Endometrial Transcriptome In Vivo

Estradiol-17β-Induced Changes in the Porcine Endometrial Transcriptome In Vivo

Prostaglandin F2α promotes embryo implantation and development in the pig

Involvement of serum glucocorticoid‐regulated kinase 1 in reproductive success

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