Prostaglandin F2α or estradiol benzoate to induce ovulation in timed artificially inseminated dairy cows

Pfeifer, Luiz Francisco Machado; Siqueira, L. G. B.; Arashiro, E. K. N.; Castro, Natália Ávila de; Viana, J. H. M.

doi:10.1590/s0100-204x2016000600005

Cited by 13 publications

(16 citation statements)

References 24 publications

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“…Our recent data, in which spayed cows were treated with 300 mg of PGF analogue (D-cloprostenol), 10.5 mg of GnRH analogue (Buserelin Acetate) or saline (0.9% of NaCl), demonstrated that only cows treated with GnRH had increasing of plasma LH concentration after treatment (not published data). Moreover, when PGF were used as ovulatory stimulus in dairy cows, it was observed an association between follicle vascularization and the time to ovulation [6]. This data led us to further hypothesize that PGF, acting mainly by a local mechanism, requires a higher developmental status of pre-ovulatory follicles compared to the systemic-mediated action of ECP.…”

Section: Discussionmentioning

confidence: 88%

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Different protocols using PGF2α as ovulation inducer in Nelore cows subjected to estradiol-progesterone timed AI based protocols

et al. 2018

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The objective of this study was to evaluate the effect of a PGFα-analogue (PGF) on ovulation and pregnancy rates after timed artificial insemination (TAI) in cattle. In Experiment 1 cows received an intravaginal progesterone-releasing device (CIDR) plus 2 mg im of estradiol benzoate (EB) on Day 0. The CIDR devices were removed on Day 8, and all cows received 150 μg im of d-cloprostenol (PGFα-analogue), 300 IU of eCG and 1 mg of estradiol cypionate (ECP) im. On Day 9, cows were randomly assigned into two groups: 1) ECP Group (n = 17), that did not receive any further treatment; and 2) ECP-PG Group (n = 14) that were given 150 μg of d-cloprostenol (PGF) as adjuvant stimulus for ovulation. No difference between groups was detected in interval for ovulation (P = 0.5), and in the proportion of cows ovulating (P = 0.09). In Experiment 2, multiparous suckling crossbred Aberdeen Angus cows (n = 260), were treated into two groups, similarly as Experiment 1; ECP group (n = 122), and ECP-PG group (n = 138). All females were TAI on Day 10. The proportion of cows treated with ECP that became pregnant was 54.9% (67/122), and cows treated with ECP plus PGF was 55.1% (76/138; P = 0.9). In Experiment 3, 686 Nelore cows, 40 to 50 days postpartum, were treated as Experiment 1 (ECP group), however, on Day 8 cows were divided into 3 groups: ECP Group (n = 216); PGF-SC Group (n = 228), in which cows did not receive ECP and were given an additional subcutaneous injection of PGF on Day 8; and PGF-IM Group (n = 242), in which cows also did not receive ECP on Day 8 and were given an additional injection of PGF im on Day 9. On Day 10, estrus was evaluated at timed AI (TAI). There was no difference in the diameter of the dominant follicle at CIDR removal and at TAI, and pregnancy per AI among groups (P > 0.05). However, the proportion of cows that displayed estrus between CIDR removal and TAI was higher in ECP group than in PGF-SC and PGF-IM groups (P < 0.001). Cows that displayed estrus has higher P/AI than cows that did not (P = 0.008). In conclusion, these results suggested that intramuscular or subcutaneous injection of PGFα could be successfully used to induce ovulation in cattle undergoing TAI, with similar pregnancy rates when compared with ECP. The subcutaneous injection of PGF on the same day of CIDR removal could be an interesting alternative due it reduces cattle management to obtain similar results.

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Section: Discussionmentioning

confidence: 88%

“…These studies support the hypothesis that the PGF can induce ovulation of dominant follicle (DF) by an independent mechanism of luteolysis. More recently, im injection of PGF has been successfully used as an ovulation inducer in TAI protocols for beef [5] and dairy cows [6].…”

Section: Introductionmentioning

confidence: 99%

Different protocols using PGF2α as ovulation inducer in Nelore cows subjected to estradiol-progesterone timed AI based protocols

et al. 2018

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“…Our recent experiment, in which ovariectomized cows were treated with a PGF analogue (d-cloprostenol), a GnRH analogue (buserelin acetate), or saline, demonstrated that only cows treated with GnRH had increased plasma LH concentration after treatment (unpublished data). Moreover, when PGF was used as an ovulatory stimulus in dairy cows, an association between follicle vascularization and the interval to ovulation was observed (Pfeifer et al, 2016). These results led us to further hypothesize that PGF, acting mainly via a local mechanism, requires a higher developmental status of pre-ovulatory follicles compared to the systemic-mediated action of ECP.…”

Section: Discussionmentioning

confidence: 86%

“…Although the number of animals included in the present study is low, these evidences lead us to infer that PGF has no ovulatory effect in buffaloes. Previous studies have indicated that d-cloprostenol has an ovulatory effect similar to that of EB in TAI protocols, resulting in similar ovulation and pregnancy rates in dairy (Pfeifer et al, 2016;Castro et al, 2018) and beef cows (Pfeifer et al, 2014(Pfeifer et al, , 2018. Moreover, the injection of PGF has also been reported to act as an ovulatory stimulus in prepubertal heifers (Pfeifer et al, 2009;Leonardi et al, 2012).…”

Section: Discussionmentioning

confidence: 95%

Prostaglandin F2? as ovulatory stimulus in dairy cows and buffaloes raised in the Amazon biome

Pfeifer¹,

Castro²,

Pereira³

et al. 2021

Semina: Ciênc. Agrár.

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This study evaluated the effect of a prostaglandin F2? (PGF) analogue as an ovulatory stimulus in dairy cows and buffaloes raised in the Amazon biome. To this end, three experiments were performed in the state of Rondônia, located in the Amazon biome. In Experiment 1, 22 lactating dairy buffaloes received 2 mg of intramuscular (I.M.) estradiol benzoate (EB) on day 0 and an intravaginal progesterone-releasing device (CIDR) from day 0 to day 9 of the protocol. On days 8 and 9, all cows were given 500 ?g of I.M. d-cloprostenol (PGF analogue). On day 10, buffaloes were divided into two groups to receive 500 ?g of PGF (PGF group, n = 8) or no treatment (CTL group, n = 14), respectively. In Experiment 2, 16 lactating crossbred dairy cows (Holstein x Gir) received 2 mg of EB on day 0 and a CIDR insert from day 0 to day 8. On days 7 and 8, all cows were given 500 ?g of d-cloprostenol. On day 9, cows were divided into two groups to receive 500 ?g of d-cloprostenol (PGF group, n = 8) or no treatment (CTL group, n = 8), respectively. In Experiment 3, 16 lactating crossbred dairy cows (Holstein x Gir) were handled and treated similarly as in Experiment 2, although cows did not receive d-cloprostenol on day 8. Single-point outcome variables were analyzed using one-way analysis of variance (ANOVA), while proportions with dichotomous outcomes were analyzed with the chi-square test. In Experiment 1, there was no difference (P = 0.30) in the ovulation rate between groups, and, on average, 68% of the buffaloes ovulated. Moreover, the treatment did not affect the interval to ovulation (P = 0.61) nor the diameter of the preovulatory follicle (P = 0.47). As for Experiment 2, only one cow, from the PG group, did not ovulate. There were no differences between the CTL and PG groups (P = 0.69) in the moment of ovulation, which occurred in average 82 h after CIDR removal. Finally, in Experiment 3, cows treated with PGF ovulated earlier than those in the CTL group (62.5 ± 5.8 and 94.5 ± 13.5 h, respectively; P = 0.05). Collectively, these results suggest that PGF hastens ovulation in lactating dairy cows, whereas no effect was observed in dairy buffaloes.

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“…Prostaglandin F 2α (PGF) analogs are commonly used to induce luteolysis in estrous synchronization programs in cattle (Pursley et al, 1995;Weems et al, 2006;Colazo & Mapletoft, 2014). However, PGF is also able to hasten and synchronize ovulation in cattle (Pfeifer et al, 2014;Pfeifer et al, 2016;Castro et al, 2017) independent of its luteolytic action (Leonardi et al, 2012). Previously, we observed that d-Cloprostenol (PGF analog) had a similar ovulatory effect to that of estradiol benzoate in timed artificial insemination (TAI) protocols, resulting in similar ovulation and pregnancy rates in dairy (Pfeifer et al, 2016;Castro et al, 2017) and beef cows (Pfeifer et al, 2014;Pfeifer et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

role of prostaglandin F2α on ovulation and LH release in cows

Castro

Leonardi

Singh

et al. 2021

Braz. J. Vet. Res. Anim. Sci.

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This study aimed to evaluate the role of prostaglandin F2α (PGF) on ovulation. In Experiment 1, cows were randomly allocated to two treatments to receive 150 μg of d-Cloprostenol (PGF Group, n = 12) or 2 mL of NaCl 0.9% (Control Group, n = 11) and CIDRs, were removed 4 days later. No cow ovulated in Control and PGF groups. In Experiment 2, cows were randomly separated into two experimental groups to receive 4 injections of 150 μg of d-Cloprostenol (n = 9) or 2 mL of NaCL 0.9% (n = 9). In this experiment, ovulation was not observed in any cows. In Experiment 3, ovariectomized cows receive three injections of 300μg of PGF analog (PGF Group, n = 5), 100μg of Lecirelin (GnRH Group, n = 5) or 2 mL of PBS (Control Group, n = 4). The LH concentration was higher (P <0.0001) in cows from the GnRH group than in the PGF and Control groups. In experiment 4, cows with preovulatory follicles (>11.5 mm) were treated with Saline (Control Group, n = 6); Lecirelin (GnRH Group, n = 7) or Cloprostenol Sodium (PGF Group, n = 6). There was a significant increase in the vascular area of follicles from 0 to 24 h in GnRH and PGF treatments. In conclusion, PGF was not able to induce ovulation in cows with high or low plasma progesterone concentration. Additionally, PGF alone was not able to induce LH release and follicle luteinization, but increased follicular vascularization.

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Prostaglandin F2α or estradiol benzoate to induce ovulation in timed artificially inseminated dairy cows

Cited by 13 publications

References 24 publications

Different protocols using PGF2α as ovulation inducer in Nelore cows subjected to estradiol-progesterone timed AI based protocols

Different protocols using PGF2α as ovulation inducer in Nelore cows subjected to estradiol-progesterone timed AI based protocols

Prostaglandin F2? as ovulatory stimulus in dairy cows and buffaloes raised in the Amazon biome

role of prostaglandin F2α on ovulation and LH release in cows

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