Prostaglandin E2 Modulates Bone Morphogenetic Protein-2 Induced Osteogenic Differentiation on a Biomimetic 3D Nanofibrous Scaffold

Liu, Yangxi; Yao, Qingqing; Sun, Hongli

doi:10.1166/jbn.2018.2490

Cited by 7 publications

(11 citation statements)

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“…3D GF scaffolds were prepared using paraffin spheres according to previous reports (Y. Liu, Yao, & Sun, ; Q. Yao, Liu, & Sun, ; Q. Yao, Liu, Tao, Baumgarten, & Sun, ) with some modifications. Briefly, 1.0 g paraffin spheres with a diameter size between 300 and 500 μm were added to Teflon molds (ϕ17‐mm cylinder) and preheated at 37°C for 28 min.…”

Section: Methodsmentioning

confidence: 99%

“…Fourier‐transform infrared spectroscopy (Thermo Scientific, Massachusetts, USA) determined the absorption on an infrared spectrum. Confocal microscopy imagining of MC3T3‐E1 in both scaffold at a cell density of 75 × 10 3 cells per scaffold were performed according to previous reports (Y. Liu et al, ). Cell‐seeded scaffold was cultured for 5 days in growth medium.…”

Section: Methodsmentioning

confidence: 99%

“…ALP enzymatic activity was quantified and qualified following previous method (Y. Liu et al, ; Miszuk et al, ; Q. Yao, Liu, Selvaratnam, Koodali, & Sun, ; Q. Yao, Liu, & Sun, ; Q. Yao et al, ). ALP staining images were taken via camera.…”

Section: Methodsmentioning

confidence: 99%

“…MC3T3‐E1 and C3H/10 T1/2 was (purchased from ATCC) used to study osteogenesis and adipogenesis. Calcium deposition measurement use total calcium LiquiColor® kit (Stanbio laboratory, Texas) as we described previously (Y. Liu et al, ). Briefly, cells/scaffolds were rinsed with DPBS and immersed in 1N hydrochloric acid and tested according to the manufacturer's instruction.…”

Section: Methodsmentioning

confidence: 99%

“…Quantitative gene expression analysis was carried out as we previously described (Y. Liu et al, ; Q. Yao, Liu, & Sun, ; Q. Yao et al, ) with some minor modifications. Briefly, total RNA was extracted using the GeneJET™ RNA Purification Kit (Thermo Scientific™, Waltham, Massachusetts).…”

Section: Methodsmentioning

confidence: 99%

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Mineralized nanofibrous scaffold promotes phenamil‐induced osteoblastic differentiation while mitigating adipogenic differentiation

Liu

Sun

2020

J Tissue Eng Regen Med

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Large bone defects represent a significant unmet medical challenge. Cost effectiveness and better stability make small molecule organic compounds a more promising alternative compared with biomacromolecules, for example, growth factors/hormones, in regenerative medicine. However, one common challenge for the application of these small compounds is their side‐effect issue. Phenamil is emerging as an intriguing small molecule to promote bone repair by strongly activating bone morphogenetic protein signaling pathway. In addition to osteogenesis, phenamil also induces significant adipogenesis based on some in vitro studies, which is a concern that impedes it from potential clinical applications. Besides the soluble chemical signals, cellular differentiation is heavily dependent on the microenvironments provided by the 3D scaffolds. Therefore, we developed a 3D nanofibrous biomimetic scaffold‐based strategy to harness the phenamil‐induced stem cell lineage differentiation. Based on the gene expression, alkaline phosphatase activity, and mineralization data, we indicated that bone‐matrix mimicking mineralized‐gelatin nanofibrous scaffold effectively improved phenamil‐induced osteoblastic differentiation, while mitigating the adipogenic differentiation in vitro. In addition to normal culture conditions, we also indicated that mineralized matrix can significantly improve phenamil‐induced osteoblastic differentiation in simulated inflammatory condition. In viewing of the crucial role of mineralized matrix, we developed an innovative and facile mineral deposition‐based strategy to sustain release of phenamil from 3D scaffolds for efficient local bone regeneration. Overall, our study demonstrated that biomaterials played a crucial role in modulating small molecule drug phenamil‐induced osteoblastic differentiation by providing a bone‐matrix mimicking mineralized gelatin nanofibrous scaffolds.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations

Mineralized nanofibrous scaffold promotes phenamil‐induced osteoblastic differentiation while mitigating adipogenic differentiation

Liu

Sun

2020

J Tissue Eng Regen Med

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Understanding and Regulating Cell-Matrix Interactions Using Hydrogels of Designable Mechanical Properties

Yang

Qin

Cheng

et al. 2021

j biomed nanotechnol

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Similar to natural tissues, hydrogels contain abundant water, so they are considered as promising biomaterials for studying the influence of the mechanical properties of extracellular matrices (ECM) on various cell functions. In recent years, the growing research on cellular mechanical response has revealed that many cell functions, including cell spreading, migration, tumorigenesis and differentiation, are related to the mechanical properties of ECM. Therefore, how cells sense and respond to the extracellular mechanical environment has gained considerable attention. In these studies, hydrogels are widely used as the in vitro model system. Hydrogels of tunable stiffness, viscoelasticity, degradability, plasticity, and dynamical properties have been engineered to reveal how cells respond to specific mechanical features. In this review, we summarize recent process in this research direction and specifically focus on the influence of the mechanical properties of the ECM on cell functions, how cells sense and respond to the extracellular mechanical environment, and approaches to adjusting the stiffness of hydrogels.

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Novel 3D embryo implantation model within macroporous alginate scaffolds

et al. 2020

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Background: Implantation failure remains an unsolved obstacle in reproductive medicine. Previous studies have indicated that estrogen responsiveness, specifically by estrogen receptor alpha (ERα), is crucial for proper implantation. There is an utmost need for a reliable in vitro model that mimics the events in the uterine wall during the implantation process for studying the regulatory mechanisms governing the process. The current twodimensional and hydrogel-based in vitro models provide only short-term endometrial cell culture with partial functionality. Results: Endometrial biopsies showed an increase in E-cadherin expression on the typical window of implantation of fertile women, compared to negligible expression in recurrent implantation failure (RIF) patients. These clinical results indicated E-cadherin as a marker for receptivity. Three-dimensional (3D) macroporous alginate scaffolds were the base for epithelial endometrial cell-seeding and long-term culture under hormone treatment that mimicked a typical menstrual cycle. The RL95-2 epithelial cell culture in macroporous scaffolds was viable for 3 weeks and showed increased E-cadherin levels in response to estrogen. Human choriocarcinoma (JAR) spheroids were used as embryo models, seeded onto cell constructs and successfully adhered to the RL95-2 cell culture. Moreover, a second model of HEC-1A with low ERα levels, showed lower E-cadherin expression and no JAR attachment. Ecadherin expression and JAR attachment were recovered in HEC-1A cells that were transfected with ERα plasmid. Conclusions: We present a novel model that enables culturing endometrial cells on a 3D matrix for 3 weeks under hormonal treatment. It confirmed the importance of ERα function and E-cadherin for proper implantation. This platform may serve to elucidate the regulatory mechanisms controlling the implantation process, and for screening and evaluating potential novel therapeutic strategies for RIF.

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Prostaglandin E2 Modulates Bone Morphogenetic Protein-2 Induced Osteogenic Differentiation on a Biomimetic 3D Nanofibrous Scaffold

Cited by 7 publications

References 0 publications

Mineralized nanofibrous scaffold promotes phenamil‐induced osteoblastic differentiation while mitigating adipogenic differentiation

Mineralized nanofibrous scaffold promotes phenamil‐induced osteoblastic differentiation while mitigating adipogenic differentiation

Understanding and Regulating Cell-Matrix Interactions Using Hydrogels of Designable Mechanical Properties

Novel 3D embryo implantation model within macroporous alginate scaffolds

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