Prostaglandin E2 levels in uterine tissues and its relationship with uterine and luteal progesterone during the estrous cycle in dairy cows

Cerbito, W.A.; Miyamoto, Akio; Balagapo, C.R.; Natural, N.G.; Miyazawa, Keiji; Sato, Kunitada

doi:10.1016/0093-691x(94)90117-2

Cited by 15 publications

(11 citation statements)

References 29 publications

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“…The same concentrations of E 2 and P 4 were maintained throughout all experiments. The concentration of these steroid hormones was chosen from the levels in the bovine uterus during the pre‐ovulatory period in situ (Cerbito et al, , Ulbrich et al, ). The purity of the epithelial cells was evaluated by immunofluorescence staining using a monoclonal antibody against cytokeratin (anti‐Cytokeratin 8 + 18; ab53280, Abcam, Tokyo, Japan) and by their characteristic epithelial morphology.…”

Section: Methodsmentioning

confidence: 99%

A proinflammatory response of bovine endometrial epithelial cells to active sperm in vitro

Elweza

Ezz

Acosta

et al. 2018

Molecular Reproduction Devel

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In the cow, cryopreserved semen is inseminated into the uterus, and most of sperm are removed by backflow and phagocytes. Nevertheless, the mechanism responsible for sperm phagocytosis is unclear. Here, we used cultured bovine uterine epithelial cells (BUECs) to investigate the uterine response to sperm and the mechanism that activates polymorphonuclear neutrophils (PMNs). BUEC monolayers were co-cultured with different numbers of washed sperm obtained from cryopreserved semen (10 , 10 , and 10 sperm/ml) for 3 hr. Sperm dose-dependently up-regulated IL8 (Interleukin 8). Sperm at 10 /ml increased mRNA expression of TNFA (Tumor necrosis factor alpha), IL1B (Interleukin 1B), NFKB2 (Nuclear factor kappa B2), and C3 (Complement factor 3), as well as PGES (Prostaglandin E synthase) expression and PGE release. Live sperm, but not dead sperm, attached to BUECs, and dead sperm did not induce an acute inflammatory response. Time-dependent effects were evaluated by co-culture of 10 /ml washed sperm with BUECs for 0, 1, 3, and 6 hr. The number of detached sperm increased gradually toward 6 hr. Maximum mRNA expression of IL8, TNFA, IL1B, and NFKB2 was induced at 3 hr, while C3 continued to increase toward 6 hr. Sperm did not stimulate mRNA expression of anti-inflammatory cytokines TGFB1 (Transforming growth factor beta 1) or IL10 (Interleukin 10). Medium conditioned by sperm co-incubated with BUECs stimulated PMNs phagocytosis of sperm in vitro. Fresh media supplemented with low levels of IL1B, TNFA, and PGE up-regulated sperm phagocytosis by PMNs as well. In conclusion, our findings strongly suggest that the active sperm attach to BUECs and trigger uterine local innate immunity with induction of a pro-inflammatory response that enhances sperm phagocytosis by PMNs.

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Section: Methodsmentioning

confidence: 99%

A proinflammatory response of bovine endometrial epithelial cells to active sperm in vitro

Elweza

Ezz

Acosta

et al. 2018

Molecular Reproduction Devel

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“…The endometrium of the uterine horn that is located on the same side of the ovary as CL has a higher progesterone (P4) concentration compared with the endometrium in the opposite uterine horn (Cerbito et al, 1994). In addition, the concentration of E2 in the oviduct ipsilateral to the preovulatory follicle is higher than that contralateral to the preovulatory follicle in bovine (Wijayagunawardane et al, 1998).…”

Section: Short Communicationmentioning

confidence: 98%

Short communication: Development of the first follicular wave dominant follicle on the ovary ipsilateral to the corpus luteum is associated with decreased conception rate in dairy cattle

Miura

Haneda

Kayano

et al. 2015

Journal of Dairy Science

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In this study, we examined the effect of the locations of the first-wave dominant follicle (DF) and corpus luteum (CL) on fertility. In total, 350 artificial insemination (AI) procedures were conducted (lactating dairy cows: n=238, dairy heifers: n=112). Ovulation was confirmed 24 h after AI. The locations of the first-wave DF and CL were examined 5 to 9d after AI using rectal palpation or transrectal ultrasonography. Lactating dairy cows and dairy heifers were divided into 2 groups: (1) the ipsilateral group (IG), in which the DF was ipsilateral to the CL; and (2) the contralateral group (CG), in which the DF was contralateral to the CL. Pregnancy was diagnosed using transrectal ultrasonography 40d after AI. Conception rates were 54.0% in all cattle: 48.9% in lactating dairy cows, and 58.9% in dairy heifers. The incidence of the first-wave DF location did not differ between IG and CG (all cattle: 184 vs. 166; lactating cows: 129 vs. 109; heifers: 55 vs. 57 for IG vs. CG). Conception rates were lower in IG than in CG (all cattle: 40.2 vs. 69.3%; lactating dairy cows: 38.0 vs. 67.0%; dairy heifers: 45.5 vs. 73.7%, for IG vs. CG). Conception rate was not affected by season or live weight in heifers and lactating cows. In addition, days in milk at AI, milk production, body condition score, and parity did not affect conception in lactating cows. In summary, development of the first-wave DF in the ovary ipsilateral to the CL was associated with reduced conception rates in both lactating cows and heifers.

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“…In addition, the study of Payne and Lamming (1994) revealed that infusion of OT to the nonpregnant uterine horn stimulated PGE2 and PGF2, synthesis. Based on the authors' previous observation of high PGE2 in early pregnancy (Cerbito et al 1994) and in this study, the low but considerable levels of PGF2, observed at early pregnancy than at luteal phase, it is speculative to say that OT may be dircctly or indirectly involved in the stimulation of certain enzymes responsible for both PGF2, synthesis andlor basal production but not release of PGF2, during the luteal phase. Uterine levels of OT, OT-R and PGF2, in the ipsilateral horn showed significantly higher levels than the contralatcral horn at follicular stage but these differences in the concentrations of OT-R and PGFZZ diminished after ovulation.…”

Section: Discussionmentioning

confidence: 75%

“…Previous reports showed significantly higher levels of P4 (Pope et al 1982;Weems el al. 1989) and PGEz (Cerbito et al 1994) in uterine tissues ipsilateral to thcCL. During luteolysis, it has been postulated that the signal emerges from the horn ipsilateral to the CL.…”

Section: Introductionmentioning

confidence: 97%

Evidence for Uterine Horn Differences in Local Concentrations of Oxytocin, Oxytocin Receptor and Prostaglandin F_2α during the Estrous Cycle and Early Pregnancy in Cows

Cerbito

Miyamoto

Sauerwein

et al. 1997

Reprod Domestic Animals

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ContentsThe current study investigated the endometrial concentration of oxytocin (OT), oxytocin receptors (OT-R) and prostaglandin Fzc, (PGF2?) ipsilateral and contralateral to the corpus luteum (CL) and their inter-relationships during the estrous cycle and early pregnancy. Reproductive tracts of 45 Holstein COWS collected from the slaughterhouse were used. Estimation of the stages of the estrous cycle was determined, based on CL characteristics and progesterone P4 levels. The luteal stage was characterized by higher OT levels in the ipsilateral horn than contralateral horn, while OT-R and PGFzl levels were low and similar in both uterine horns. At follicular stage, thc ipsilateral horn showed significantly higher concentrations of OT, OT-R and PGF2, than the contralateral horn. At post-ovulation, OT levels remained high and significantly higher in the ipsilateral horn. The OT-R and PGF2, levels decreased to a certain level but were not significantly different from their levels at the follicular stage, and no difference was found between horns. At early pregnancy, OT levels were low, OT-R levels were significantly lower in the ipsilateral than in the contralateral horn. and PGF2, decreased, but the levels were higher than at luteal phase. The results indicate clear stage-dependent changes of endocrine parameters within ipdateral and contralateral horn in relation to the CL and suggest that the horn adjacent to CL is more active, sensitive and responsive to the cause and effect of luteolysis, supporting the concept of a utero-ovarian local regulating mechanism. Further, the significantly low OT-R in the ipsilateral horn suggests a local effect of the CL or the embryo. Inhalt Unterschiedliche lokale Konzentrationen von Oxytocin, Oxytocin-Rezeptor und Prostaglandin FZx in den Uterushiirnern bei zyklischen und friihtragenden KuhenIn der vorleigenden Untersuchung wurde die endometrische Konzentration von Oxytocin (OT), Oxytocin-Rezeptor (OT-R) und Prostaglandin F2, (PGF2,) im ipsi-und kontralateralen Horn zum Corpus luteum (CL) untersucht und die Abhlngigkeit wahrend der Brunst und der friihen Trachtigkeit bestimmt. Es wurden Genitalorgane (Schlachtorgane) von 45 HolsteinKiihen venvendet. Die Schatzung des Zyklusstadiums basicrte auf den Merkmalen der Gelbkorper-und der P3 Level. In der Gelbkorperphase waren charakteristisch hohcre OT-Spiegel im ipsilateralen Horn als im kontralateralen Horn zu finden, wohingegen OT-R und PGF2, in beiden Hornern gering waren. In der Follikelphase wies das ipsilaterale Horn im Vergleich zum kontralateralen signifikant hohere Konzentrationen von OT, OT-R und PGF2, auf. Postovulatorisch verblieben die OTWerte auf hoherem Niveau und waren im ipsilateralcn Horn signifikant hoher. OT-R-und PGF2,-Werte sanken bis auf ein bestimmtes Niveau, aber waren nicht signifikant unterschiedlich zu den entsprechenden Werten der Follikelphase. Zwischen den beiden Hornern wurden keine Unterschiede gefunden. Wahrend der friihen Trachtigkeit waren die OTWerte niedrig, die OT-R-Level signifikant geringer im...

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Prostaglandin E2 levels in uterine tissues and its relationship with uterine and luteal progesterone during the estrous cycle in dairy cows

Cited by 15 publications

References 29 publications

A proinflammatory response of bovine endometrial epithelial cells to active sperm in vitro

A proinflammatory response of bovine endometrial epithelial cells to active sperm in vitro

Short communication: Development of the first follicular wave dominant follicle on the ovary ipsilateral to the corpus luteum is associated with decreased conception rate in dairy cattle

Evidence for Uterine Horn Differences in Local Concentrations of Oxytocin, Oxytocin Receptor and Prostaglandin F_2α during the Estrous Cycle and Early Pregnancy in Cows

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Prostaglandin E2 levels in uterine tissues and its relationship with uterine and luteal progesterone during the estrous cycle in dairy cows

Cited by 15 publications

References 29 publications

A proinflammatory response of bovine endometrial epithelial cells to active sperm in vitro

A proinflammatory response of bovine endometrial epithelial cells to active sperm in vitro

Short communication: Development of the first follicular wave dominant follicle on the ovary ipsilateral to the corpus luteum is associated with decreased conception rate in dairy cattle

Evidence for Uterine Horn Differences in Local Concentrations of Oxytocin, Oxytocin Receptor and Prostaglandin F2α during the Estrous Cycle and Early Pregnancy in Cows

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Product

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Evidence for Uterine Horn Differences in Local Concentrations of Oxytocin, Oxytocin Receptor and Prostaglandin F_2α during the Estrous Cycle and Early Pregnancy in Cows