Prostaglandin E2 Induces Skin Aging via E-Prostanoid 1 in Normal Human Dermal Fibroblasts

Shim, Joong-Hyun

doi:10.3390/ijms20225555

Cited by 15 publications

(12 citation statements)

References 41 publications

(50 reference statements)

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“…A possible explanation for the downregulation of EP2 receptor is receptor desensitization, which is an indirect evidence of PGE2 rise. Interestingly, we found instances in the literature which contradict the above pattern of EP receptor mRNA expression; PGE2 caused the downregulation of EP4 and the upregulation of EP2 receptor in macrophages and fibroblasts (Ikegami et al, 2001;Shim, 2019). Therefore, the gene expressions of EP2 and EP4 are differentially regulated, the direction of change in gene expression probably depends on the trigger.…”

Section: Discussionmentioning

confidence: 73%

Cyclooxygenase-2 Modulates Glycosaminoglycan Production in the Skin During Salt Overload

et al. 2020

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Sodium (Na +) can accumulate in the skin tissue, sequestered by negatively charged glycosaminoglycans (GAGs). During dietary salt overload, the amount and charge density of dermal GAG molecules-e.g., hyaluronic acid (HA) and chondroitin sulfate (CS)increases; however, the regulation of the process is unknown. Previously, it has been demonstrated that the level of cyclooxygenase-2 (COX-2) activity and the content of prostaglandin E2 (PGE2) are elevated in the skin due to high-salt consumption. A link between the COX-2/PGE2 system and GAG synthesis was also suggested. We hypothesized that in dermal fibroblasts (DFs) high-sodium concentration activates the COX-2/PGE2 pathway and also that PGE2 increases the production of HA. Our further aim was to demonstrate that the elevation of the GAG content is ceased by COX-2 inhibition in a salt overloaded animal model. For this, we investigated the messenger RNA (mRNA) expression of COX-2 and HA synthase 2 enzymes as well as the PGE2 and HA production of DFs by real-time reverse transcription PCR (qRT-PCR) and ELISA, respectively. The results showed that both high-sodium concentration and PGE2 treatment increases HA content of the media. Sodium excess activates the COX-2/PGE2 pathway in DFs, and COX-2 inhibition decreases the synthesis of HA. In the animal experiment, the HA-and CS disaccharide content in the skin of male Wistar rats was measured using high performance liquid chromatography-mass spectrometry (HPLC-MS). In the skin of rats receiving high-salt diet, the content of both HA-and monosulfated-CS disaccharides increased, whereas COX-2 inhibition blocked this overproduction. In conclusion, high-salt environment could induce GAG production of DFs in a COX-2/PGE2-dependent manner. Moreover, the COX-2 inhibition resulted in a decreased skin GAG content of the salt overloaded rats. These data revealed a new DF-mediated regulation of GAG synthesis in the skin during salt overload.

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Section: Discussionmentioning

confidence: 73%

Cyclooxygenase-2 Modulates Glycosaminoglycan Production in the Skin During Salt Overload

et al. 2020

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“…In skin aging, COX-2 acts as a mediator for the biosynthesis of prostaglandin E2, which contributes to the production of MMPs. IL-1 and IL-6 are regulators that contribute to skin wrinkle formation, including inhibition of collagen biosynthesis [ 59 ]. Luteolin, a flavonoid isolated and reported from various natural products, suppresses ROS and pro-inflammatory mediators and promotes photoaging of the epidermis and dermis to reduce IL-6, IL-22, IL-17, and COX-2 [ 46 ].…”

Section: Discussionmentioning

confidence: 99%

Improvement of Damage in Human Dermal Fibroblasts by 3,5,7-Trimethoxyflavone from Black Ginger (Kaempferia parviflora)

et al. 2022

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Reactive oxygen species (ROS) are generated during intrinsic (chronological aging) and extrinsic (photoaging) skin aging. Therefore, antioxidants that inhibit ROS production may be involved in delaying skin aging. In this study, we investigated the potential effects of compounds isolated from black ginger, Kaempferia parviflora, a traditional medicinal plant, on normal human dermal fibroblasts in the context of inflammation and oxidative stress. The isolated compounds were structurally characterized as 5-hydroxy-7-methoxyflavone (1), 3,7-dimethoxy-5-hydroxyflavone (2), 5-hydroxy-3,7,3,4-tetramethoxyflavone (3), 7,4-dimethylapigenin (4), 3,7,4-trimethylkaempferol (5), and 3,5,7-trimethoxyflavone (6), using nuclear magnetic resonance spectroscopy (NMR) and liquid chromatography–mass spectrometry (LC/MS) analyses. These flavonoids were first evaluated for their ability to suppress extracellular matrix degradation in normal human dermal fibroblasts. Of these, 3,5,7-trimethoxyflavone (6) significantly inhibited the tumor necrosis factor (TNF)-α-induced high expression and secretion of matrix metalloproteinase (MMP)-1 by cells. We further found that 3,5,7-trimethoxyflavone suppressed the excessive increase in ROS, mitogen-activated protein kinases (MAPKs), Akt, and cyclooxygenase-2 (COX-2)and increased heme oxygenase (HO)-1 expression. The expression of pro-inflammatory cytokines, including interleukin (IL)-1β, IL-6, and IL-8, was also suppressed by 3,5,7-trimethoxyflavone (6). Taken together, our results indicate that 3,5,7-trimethoxyflavone (6) isolated from K. parviflora is a potential candidate for ameliorating skin damage.

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“…It should be remembered that PGE2 is a critical pro-inflammatory mediator responsible for hyperalgesia, which stimulates the production of pro-inflammatory cytokines. On the other hand, at least in theory should reactivate the resolution and biosynthesis programs of SPM-lipoxins, Rvs, PD and maresins within inflammatory exudates [ 108 ].…”

Section: The Link Between Psoriasis and Obesitymentioning

confidence: 99%

Exploring the Links between Obesity and Psoriasis: A Comprehensive Review

Barros

Durán

Vera

et al. 2022

IJMS

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Obesity is a major public health issue worldwide since it is associated with the development of chronic comorbidities such as type 2 diabetes, dyslipidemias, atherosclerosis, some cancer forms and skin diseases, including psoriasis. Scientific evidence has indicated that the possible link between obesity and psoriasis may be multifactorial, highlighting dietary habits, lifestyle, certain genetic factors and the microbiome as leading factors in the progress of both pathologies because they are associated with a chronic pro-inflammatory state. Thus, inflammation management in obesity is a plausible target for psoriasis, not only because of the sick adipose tissue secretome profile but also due to the relationship of obesity with the rest of the immune derangements associated with psoriasis initiation and maintenance. Hence, this review will provide a general and molecular overview of the relationship between both pathologies and present recent therapeutic advances in treating this problem.

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Prostaglandin E2 Induces Skin Aging via E-Prostanoid 1 in Normal Human Dermal Fibroblasts

Cited by 15 publications

References 41 publications

Cyclooxygenase-2 Modulates Glycosaminoglycan Production in the Skin During Salt Overload

Cyclooxygenase-2 Modulates Glycosaminoglycan Production in the Skin During Salt Overload

Improvement of Damage in Human Dermal Fibroblasts by 3,5,7-Trimethoxyflavone from Black Ginger (Kaempferia parviflora)

Exploring the Links between Obesity and Psoriasis: A Comprehensive Review

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