Prostaglandin E2 Hastens Oviductal Transport of Equine Embryos1

Weber, J.A.; Freeman, D.A.; Vanderwall, Dirk K.; Woods, G.L.

doi:10.1095/biolreprod45.4.544

Cited by 116 publications

(52 citation statements)

References 11 publications

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“…The PGF 2 receptors in the adult rat oviduct predominated during the periovulatory period (Orlicky & Williams-Skipp 1993) and the response of the oviduct to PGF 2 increased markedly following ovulation in marmoset monkeys (Barbosa et al 1980). Also, PGE 2 treatment hastened the oviduct transport of equine embryos (Weber et al 1991). The direct stimulation of the amplitude of oviduct contraction by PG during the follicular and postovulatory phases observed in this study may be due to the increased sensitivity as a result of an increase in the number of binding sites during this period.…”

Section: Discussionmentioning

confidence: 49%

In vitro regulation of local secretion and contraction of the bovine oviduct: stimulation by luteinizing hormone, endothelin-1 and prostaglandins, and inhibition by oxytocin

Wijayagunawardane

Miyamoto²,

Taquahashi³

et al. 2001

Journal of Endocrinology

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The precise regulatory mechanisms of cyclic oviductal contraction in the cow are unclear. The purpose of this study was to evaluate the effect of luteinizing hormone (LH), steroids, prostaglandins (PGs) and peptides on the oviductal contraction and secretion of PGs and endothelin (ET-1). In addition, the cyclic expression of mRNA for ET-1 and its receptors (ET-R) was evaluated by reverse transcription-polymerase chain reaction (RT-PCR). In the in vitro microdialysis study, an infusion of LH alone or in combination with progesterone (P 4 ), estradiol-17 (E 2 ) and/or ET-1 stimulated pronounced release of PGE 2 , PGF 2 and ET-1 in the oviducts from cows in the follicular and postovulatory phases. The addition of LH, LH+P 4 +E 2 and/or ET-1 to the medium increased the amplitude of oviductal contraction. However, oxytocin (OT) completely blocked the responses of oviductal secretion and contraction. In contrast, these substances did not show any effect in the oviducts from cows in the mid luteal phase. Similar expression patterns of mRNA encoding for ET-R type A and type B were found, which were highest during the postovulatory phase, lower during the luteal phase, with the lowest expression during the follicular phase. We suggest that the preovulatory LH surge, together with increasing E 2 levels from the Graafian follicle and a basal P 4 from regressing corpora lutea (CL), stimulates maximum oviductal production of PG and ET-1, resulting in oviductal contraction for a rapid transport of gametes. OT released from the newly-formed CL may block these mechanisms, and slow contractions for transport of the embryo to the uterus.

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Section: Discussionmentioning

confidence: 49%

In vitro regulation of local secretion and contraction of the bovine oviduct: stimulation by luteinizing hormone, endothelin-1 and prostaglandins, and inhibition by oxytocin

Wijayagunawardane

Miyamoto²,

Taquahashi³

et al. 2001

Journal of Endocrinology

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“…with oviductal transport [Weber et al, 1991a;1992a]; and 3) intraoviductal PGE 2 treatment of bred mares has been found to hasten oviductal transport [Weber et al, 1991b].…”

Section: Introductionmentioning

confidence: 98%

Relaxatory Effect of Prostaglandin E2 on Circular Smooth Muscle Isolated from the Equine Oviductal Isthmus1

Weber

Woods

Lichtenwalne

1995

Biology of Reproduction

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Our objectives were to test whether or not prostaglandin E 2 (PGE 2 ) relaxes equine isthmic circular smooth muscle in vitro, and to determine the effects of surgery, time postexcision, and source of tissue on isthmic responsiveness to PGE 2 . Oviducts were surgically collected after general anesthesia from four research herd mares on Day 5 postovulation, after stunning and exsanguination from four research herd mares on Day 5, and from seven slaughterhouse mares during diestrus. Circular smooth muscle contractions were measured in vitro with an isometric force transducer. Tissues were precontracted with 2 |iM histamine and then were treated with PGE 2 (0, 0.1, 1, 10, 100, or 1000 nM). Histamine treatment significantly (p < 0.001) increased tissue tension (500.0 ± 0.0 to 818.9 ± 47.9 mg) and contractile frequency (13.3 ± 0.4 to 29.8 ± 1.6 contractions/min), and decreased contractile amplitude (69.7 ± 11.9 to 23.7 ± 4.2 mg). PGE 2 treatment significantly (p < 0.001) decreased tissue tension and contractile frequency and significantly (p < 0.001) increased contractile amplitude of histamine-precontracted tissue in a dose-dependent manner. Tissue obtained from mares anesthetized on Day 5 was significantly (p < 0.05) less responsive to PGE 2 than tissue obtained from mares killed on Day 5. Responsiveness to PGE 2 of tissue from mares killed on Day 5 did not decrease significantly after 12 h of storage at 4°C. These results support the hypothesis that PGE 2 relaxes isthmic circular smooth muscle, and indicate that anesthetic agents reduce isthmic responsiveness to PGE 2 .

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“…Data on the use of PGE2 in mares are scarce and have been limited to local therapy in the follicle (Martínez-Boví and Cuervo-Arango 2014), oviduct (Weber et al 1991, Allen et al 2006, uterus (Weber et al 1991), and cervix (Rigby et al 1998). To the author's best knowledge, only one published study has reported the intramuscular treatment of 10 mg of PGE2 in six mares as a single bolus (Weber et al 1991).…”

Section: Introductionmentioning

confidence: 99%

“…To the author's best knowledge, only one published study has reported the intramuscular treatment of 10 mg of PGE2 in six mares as a single bolus (Weber et al 1991). However, no mention of side effects following the systemic administration of PGE2 was made.…”

Section: Introductionmentioning

confidence: 99%

The role of PGE2 and PGF2a in follicle wall rupture and their implications in the development and treatment of luteinized unruptured follicles

Cuervo‐Arango¹,

Martínez-Boví²

2016

PHK

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Summary:The article summarizes the results of several studies, which highlight the relevance of prostaglandins E2 (PGE2) and F2 (PGF2a) during the ovulatory process in mares and their implication in the pathogenesis of luteinized unruptured follicles. Following the preovulatory luteinizing hormone (LH) surge, the two prostaglandins are synthesized in granulosa cells. They subsequently trigger a cascade of events that lead to ovulation. The exogenous administration of intrafollicular PGE2 and PGF2a can induce follicle rupture and ovulation within 12 h of injection, even in mares in early estrus, and before the beginning of the abrupt rise of the preovulatory LH surge. The systemic administration of a prostaglandin synthesis inhibitor (flunixin-meglumine) during the periovulatory period blocks ovulation and induces the development of luteinized unruptured follicles (LUFs). LUFs share similar ultrasonographic and hormonal characteristics with hemorragic anovulatory follicles (HAFs). The administration of intrafollicular PGE2 and PGF2a in mares treated with a prostaglandin synthesis inhibitor restores normal ovulation and allows oocyte release and fertilization. However, the systemic administration of prostaglandins does not appear to have any effect on hastening ovulation.

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Prostaglandin E2 Hastens Oviductal Transport of Equine Embryos1

Cited by 116 publications

References 11 publications

In vitro regulation of local secretion and contraction of the bovine oviduct: stimulation by luteinizing hormone, endothelin-1 and prostaglandins, and inhibition by oxytocin

In vitro regulation of local secretion and contraction of the bovine oviduct: stimulation by luteinizing hormone, endothelin-1 and prostaglandins, and inhibition by oxytocin

Relaxatory Effect of Prostaglandin E2 on Circular Smooth Muscle Isolated from the Equine Oviductal Isthmus1

The role of PGE2 and PGF2a in follicle wall rupture and their implications in the development and treatment of luteinized unruptured follicles

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