Prostaglandin E2 9-keto reductase activity in bovine retained and not retained placenta

Kankofer, Marta; Wierciński, J; Zerbe, Holm

doi:10.1054/plef.2002.0367

Cited by 13 publications

(7 citation statements)

References 28 publications

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“…In the present study, among identified proteins were also prostaglandin reductase, which is crucial for the reverse shift between prostaglandins of series E and F and can be involved in the process of physiological and altered separation of placenta as it was described in cows (Kankofer, Wierciński, & Zerbe, ). Dehydrogenase/reductase SDR family member 4 known as NADPH‐dependent retinol dehydrogenase/reductase (1.1.1.184) reduces all‐trans‐retinal and 9‐cis retinal.…”

Section: Discussionsupporting

confidence: 66%

“…In the present study, among identified proteins were also prostaglandin reductase, which is crucial for the reverse shift between prostaglandins of series E and F and can be involved F I G U R E 5 Chromatograms of endometrium and allantochorion from FMR and control mares, (n = 8 -control, n = 6 -FMR) F I G U R E 6 Histograms of significantly different proteins from endometrium of FMR and control mares as detected in LC-MS analysis in the process of physiological and altered separation of placenta as it was described in cows (Kankofer, Wierciński, & Zerbe, 2002 Falco, 2012), but also regulator of pregnancy. Its abundance was higher in FMR mares.…”

Section: Discussionmentioning

confidence: 99%

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The preliminary studies on protein profile in retained and not retained foetal membranes in heavy draft mares

Wawrzykowski

Rapacz-Leonard

Wiącek

et al. 2019

Reprod Domestic Animals

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Protein profile of the placenta expresses its function and maintenance. Any alterations can be reflected in qualitative and quantitative changes in this profile. The aim of the present study was the evaluation of protein profile in the placenta of mares suffering from the retention of foetal membranes (FMR) by two separation methods and the comparison with physiologically released tissues. Placentas from 14 healthy, heavy draft mares were collected immediately after the expulsion of newborn. Tissues after homogenization and staining with fluorescent dyes were subjected to electrophoretic as well as chromatographic separation. Electrophoretic gels were statistically analysed for the presence and abundance of examined proteins, while some proteins were identified in chromatographic fractions. Out of 248 spots detected in endometrium, 38 differed significantly between FMR and control animals, while in allantochorion, respective values reached 241 and 27 spots (p < .05). Among identified proteins that expressed higher abundance in endometrium of FMR mares than control animals were prostaglandin reductase, dehydrogenase/reductase SDR family, and placental growth factor. These proteins are involved in regulation of parturition. Additionally, the following proteins responsible for physiological activity of a cell—guanine methyl transferase, aspartyl/asparaginyl beta‐hydroxylase and GTP‐binding protein, were identified. These proteins expressed higher abundance in allantochorion of FMR mares than in controls. This preliminary study confirmed the disturbances in protein pattern between foetal membranes in FMR and healthy mares. Further qualitative and quantitative experiments are necessary to deepen our knowledge on the mechanisms of the retention of foetal membranes in mares.

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Section: Discussionsupporting

confidence: 66%

“…In the present study, among identified proteins were also prostaglandin reductase, which is crucial for the reverse shift between prostaglandins of series E and F and can be involved F I G U R E 5 Chromatograms of endometrium and allantochorion from FMR and control mares, (n = 8 -control, n = 6 -FMR) F I G U R E 6 Histograms of significantly different proteins from endometrium of FMR and control mares as detected in LC-MS analysis in the process of physiological and altered separation of placenta as it was described in cows (Kankofer, Wierciński, & Zerbe, 2002 Falco, 2012), but also regulator of pregnancy. Its abundance was higher in FMR mares.…”

Section: Discussionmentioning

confidence: 99%

The preliminary studies on protein profile in retained and not retained foetal membranes in heavy draft mares

Wawrzykowski

Rapacz-Leonard

Wiącek

et al. 2019

Reprod Domestic Animals

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“…Furthermore, 9K‐PGR's potential has been observed in the ovine endometrium and CL tissue (Beaver & Murdoch, ). In addition, the activity of 9K‐PTGR was also confirmed in the bovine placenta (Kankofer & Wierciński, ) and endometrium (Asselin & Fortier, ). The high level of PTGF during the late‐luteal phase and especially during luteolysis in our study could also be influenced by the 9K‐PTGR activity.…”

Section: Discussionmentioning

confidence: 81%

Changes in the expression of prostaglandin family members in bovine corpus luteum during the estrous cycle and pregnancy

Berisha

Schams

Rodler

et al. 2018

Molecular Reproduction Devel

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The aim of this study was to characterize certain prostaglandin family members in the bovine corpus luteum (CL) during the estrous cycle and pregnancy. The CL tissue was assigned to the stages 1-2, 3-4, 5-7, 8-12, 13-16 and >18 days (after regression) of the estrous cycle and 1-2, 3-4, 6-7, and >8 months of pregnancy. In these samples, we investigated prostaglandin F2alpha (PTGF), prostaglandin E2 (PTGE), their receptors (PTGFR, PTGER2, and PTGER4), cyclooxygenase 2 (COX-2), PTGF synthase (PTGFS), and PTGE synthase (PTGES). The expression of messenger RNA (mRNA) was measured by reverse transcription quantitative polymerase chain reaction, hormones by enzyme immunoassay, and localization by immunohistochemistry. The mRNA expression of COX-2, PTGFS, and PTGES in CL during the early-luteal phase was high followed by a continuous and significant downregulation afterward, as well as during all phases of pregnancy. The concentration of PTGF in CL tissue was high during the early-luteal phase, decreased significantly in the mid-luteal phase, and increased again afterward. In contrast, the concentration of PTGE increased significantly during the late-luteal phase followed by a decrease during regression. The PTGE level increased again during late pregnancy. Immunohistochemically, the large granulose-luteal cells show strong staining for COX-2 and PTGES during the early-luteal stage followed by lower activity afterward. During pregnancy, most of the luteal cells were only weakly positive or negative. In conclusion, our results indicate that the examined prostaglandin family members are involved in the local mechanisms that regulate luteal function, specifically during CL formation, function, and regression and during pregnancy in the cow.

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“…PGE 2 9‐keto‐reductase is the enzyme involved in the reversible metabolism of PGF 2α into PGE 2 and it regulates the ratio of these two hormones. Its activity is significantly higher in both the fetal and maternal compartments of placentomes obtained from cows that have retained fetal membranes than in normal healthy cows (Kankofer and others 2002). This finding is consistent with a proposal made almost 20 years earlier by Gross and others (1987) that after pharmacological induction of parturition, retained fetal membranes might be prevented by administering PGF 2α to the cows immediately after parturition; this procedure reduced the incidence of retained fetal membranes from 91 per cent to 9 per cent.…”

Section: Anatomy Of the Fetomaternal Unitmentioning

confidence: 99%

Structure and function of the bovine fetomaternal unit in relation to the causes of retained fetal membranes

McNaughton

Murray

2009

Veterinary Record

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Approximately 5 per cent of dairy cows are affected by retained fetal membranes. Retained fetal membranes are an important risk factor for the metritis/endometritis disease complex occurring in early lactation, and costs the UK dairy industry approximately pound16 million annually in lost production. Veterinary clinicians have described the clinical signs, treatment and some associated risk factors of the condition since early Victorian times, and these have not changed over time. Research carried out within the past 20 years suggests that there is an immunological basis for retained fetal membranes. In a normal calving, the maturation of the placenta and its separation from healthy caruncles depends upon incompatibility between maternal and fetal major histocompatibility complex class I expressed on epithelium within the fetomaternal unit. Placental maturation follows stimulation of the maternal immune response and the production of neutrophil-activating factors within the epithelium in the caruncular arcade. This affects the extracellular matrix components within the placentome, breaking down collagen within the chorionic villi and assisting in separation from the caruncle. Factors influencing normal placental maturation include downregulation of antioxidative defence mechanisms against reactive oxygen species, a lower ratio of prostaglandin E(2) to prostaglandin F(2alpha) within the fetomaternal compartments of the placentome, and an elevated steroid hormone receptor status, which reduces the rate of apoptosis occurring in the chorionic epithelium before calving.

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Prostaglandin E2 9-keto reductase activity in bovine retained and not retained placenta

Cited by 13 publications

References 28 publications

The preliminary studies on protein profile in retained and not retained foetal membranes in heavy draft mares

The preliminary studies on protein profile in retained and not retained foetal membranes in heavy draft mares

Changes in the expression of prostaglandin family members in bovine corpus luteum during the estrous cycle and pregnancy

Structure and function of the bovine fetomaternal unit in relation to the causes of retained fetal membranes

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