Prostaglandin E-2 as a potential luteotrophic agent during early pregnancy in cattle

Shelton, K.; Parkinson, T. J.; Hunter, M. G.; Kelly, Rodney W.; Ge, Lan

doi:10.1530/jrf.0.0900011

Cited by 31 publications

(24 citation statements)

References 32 publications

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“…Our results are consistent with other studies in utero, as the total amount of PGs were over three times higher in Day 13 pregnant after multiple ET than in cyclic heifers (Spencer et al, 2013). In particular, concentrations of PGF 2 α normally exceed several times that of PGE 2 (Bartol et al, 1981;Lewis et al, 1982;Shelton et al, 1990;Ulbrich et al, 2009). At such stages, PGs activate a number of IFNT-stimulated genes, and it seems that PGs could be important recognition signalling molecules in cattle (Spencer et al, 2013;Lonergan and Forde, 2014).…”

Section: Discussionsupporting

confidence: 93%

PGF2α levels in Day 8 blood plasma are increased by the presence of one or more embryos in the uterus

Gómez

Martín

Carrocera

et al. 2015

Animal

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In cattle, the detection of very early endometrial responses is considered to be hampered by the presence of only a single embryo. Therefore, we have previously developed a model of multiple embryo transfer to circumvent this hindrance. In this work, we analysed embryo-maternal interactions in the bovine uterus on day 8 of development while comparing the presence of multiple v. single embryos using embryo transfer and artificial insemination, respectively. Concentration of proteins (β-actin, NFkB, clusterin and immunoproteosome 20S β5i subunit-i20S), by western blot, and hexoses (glucose and fructose) were measured in paired samples of uterine fluid (UF) from the same animal with and without embryos in the uterus and were compared with UF obtained after artificial insemination. Prostaglandin (PG) F 2 α and PGE 2 concentrations were also analysed in blood plasma. The four proteins analysed and hexoses were unaffected by the presence of one or more embryos in the uterus. However, blood PGF 2 α showed similar, significant increases with one or more embryos over cyclic animals; such changes were not observed in blood PGE 2 . Although multiple embryo transfer may appear to be non-physiological, we showed that the uterus, at the very early embryonic stages, does exhibit physiological reactions. Multiple embryo transfer can, therefore, be used for studies of very early embryomaternal interactions in vivo in monotocous species.

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Section: Discussionsupporting

confidence: 93%

PGF2α levels in Day 8 blood plasma are increased by the presence of one or more embryos in the uterus

Gómez

Martín

Carrocera

et al. 2015

Animal

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“…Different superscript letters indicate significant differences between groups (P!0.05). Bartol et al 1981, Lewis et al 1982, Shelton et al 1990. Highest levels of PGF 2a and PGE 2 opposed to low levels of PGD 2 , TXB 2 , and 6-keto PGF 1a were found in ovine luminal cell supernatants treated with [ 3 H]AA, whereby the latter authors specifically point out that exogenous [ 3 H]AA may not reflect endogenous synthesis capabilities (Charpigny et al 1999).…”

Section: Discussionmentioning

confidence: 98%

“…PG released from the endometrium not only reach blood vessels, but also appear in the uterine lumen. Early studies have demonstrated PGF 2a and PGE 2 in in vivo flushings (Ellinwood et al 1979, Bartol et al 1981, Shelton et al 1990. Additionally, the conversion Figure 3 The concentrations of the prostaglandins and metabolites vary with respect to day and status in uterine flushings.…”

Section: Discussionmentioning

confidence: 99%

Quantitative characterization of prostaglandins in the uterus of early pregnant cattle

Ulbrich¹,

Schülke²,

Groebner³

et al. 2009

Reproduction

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Prostaglandins (PGs) are important regulators of reproductive processes including early embryonic development. We analyzed the most relevant PG in bovine uteri at different preimplantation pregnancy stages when compared with non-pregnant controls. Additionally, endometrium and trophoblast tissues were examined regarding specific enzymes and receptors involved in PG generation and function. Simmental heifers were artificially inseminated or received seminal plasma only. At days 12, 15, or 18, post-estrus uteri were flushed for PG determination by liquid chromatography-tandem mass spectrometry. Endometrium and trophoblast tissues were sampled for RNA extraction and quantitative real-time PCR analysis. At all days and points of time examined, the concentration of 6-keto PGF 1a (stable metabolite of PGI 2 ) was predominant followed by PGF 2a OPGE 2 OPGD 2 zTXB 2 (stable metabolite of TXA 2 ). At days 15 and 18, PG increased from overall low levels at day 12, with a much more pronounced increase during pregnancy. The PGF 2a /PGE 2 ratio was not influenced by status. The highest PG concentration was measured at day 15 with 6-keto PGF 1a (6.4 ng/ml) followed by PGF 2a (1.1 ng/ml) and PGE 2 (0.3 ng/ml). Minor changes in endometrial PG biosynthesis enzymes occurred due to pregnancy. Trophoblasts revealed high transcript abundance of general and specific PG synthases contributing to uterine PG. As PGI 2 and PGF 2a receptors were abundantly expressed by the trophoblast, abundant amounts of PGI 2 and PGF 2a in the uterine lumen point towards an essential role of PG for the developing embryo. High amounts of PG other than PGE 2 in the preimplantation uterus may be essential rather than detrimental for successful reproduction.

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“…The production of prostaglandin F2 (PGF2) is responsible for luteolysis and prostaglandin E 2 (PGE 2 ) is involved in maternal recognition of pregnancy (Arosh et al 2002;Shelton et al 1990) and as such these pathways are of major importance in early embryonic development. Triglycerides are not only important in membrane synthesis but are also suggested to have a role in early embryonic development as an energy source (Ferguson and Leese 2006;Forde et al 2009a).…”

Section: Discussionmentioning

confidence: 99%

Characterisation of endometrial gene expression and metabolic parameters in beef heifers yielding viable or non-viable embryos on Day 7 after insemination

Beltman

Forde

Furney

et al. 2010

Reprod. Fertil. Dev.

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Abridged title: Endometrial gene expression and metabolic parameters in subfertile beef heifers. AbstractThe objective of this study was to compare the hormonal, metabolic characteristics and endometrial gene expression profiles in beef heifers yielding either a viable or a degenerate embryo on Day 7 after insemination as a means to explain differences in embryo survival.Oestrus was synchronised in cross bred beef heifers (n=145) using a CIDR-prostaglandin protocol and heifers (n=102) detected in standing oestrus (within 24-48 h post CIDR removal) were inseminated 12-18 h after detection of oestrus (Day 0) with frozen-thawed semen from a single ejaculate of a bull with proven fertility. Blood samples were collected from Day 4 to Day 7 post oestrus to measure progesterone (on Days 4, 5 and 7), insulin and IGF-I (on Days 4 and 6) and urea concentrations (on Day 7). All animals were slaughtered on Day 7. Uterine pH was also determined. Animals from which an embryo was recovered were classified as either having a viable embryo (morula/blastocyst stage); n=32, or a retarded embryo (arrested at 2-to 16-cell stage); n=19. The overall recovery rate was 64%. There was no significant difference in blood parameters measured or uterine pH at slaughter between the heifers with either a viable or retarded embryo. The relative abundance of 9 transcripts out of 53 tested from the endometrial tissue was different between heifers with a viable embryo or retarded embryo: MOGAT1, PFKB2, LYZ2, SVS8, UHRF1, PTGES, AGPAT4, DGKA and HGPD. Both LYZ2 and UHRF1 are associated with regulation of the immune system. PFKFB2 is a mediator in glycolysis and MOGAT, AGPAT4 and DGKA belong to the triglyceride synthesis pathway. PTGES and HGPD belong to the prostaglandin pathway.Both of these metabolic pathways are important for early embryonic development. In conclusion, retarded embryo development in this study was not related to serum progesterone, IGF-I, insulin or urea concentrations nor to uterine pH at slaughter. However, altered expression of genes involved in the prostaglandin and triglyceride pathways, as well as 2 genes that are closely associated with the regulation of immunity in the endometrium may indicate a uterine component to the retardation of the embryos in these beef heifers.

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Prostaglandin E-2 as a potential luteotrophic agent during early pregnancy in cattle

Cited by 31 publications

References 32 publications

PGF2α levels in Day 8 blood plasma are increased by the presence of one or more embryos in the uterus

PGF2α levels in Day 8 blood plasma are increased by the presence of one or more embryos in the uterus

Quantitative characterization of prostaglandins in the uterus of early pregnant cattle

Characterisation of endometrial gene expression and metabolic parameters in beef heifers yielding viable or non-viable embryos on Day 7 after insemination

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