Prostaglandin D2 metabolite stimulates collagen synthesis by human osteoblasts during calcification

Tasaki, Yukari; Takamori, R; Koshihara, Yasuko

doi:10.1016/0090-6980(91)90001-v

Cited by 45 publications

(31 citation statements)

References 14 publications

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“…In addition, procollagen I mRNA was stimulated at high cell density [18]. In human bone cells cultivated from periosteum, 10 −8 M 1,25(OH) 2 D 3 stimulated collagen I synthesis and mRNA expression to the same extent after confluence [49]. In immortalized human bone marrow cells, 1,25(OH) 2 D 3 stimulated collagen mRNA and protein secretion into the same direction as proliferating and differenti- Stage I means 50% confluence, stage II 75%, stage III 100%, and stage IV 7 days 100% confluence.…”

Section: Discussionmentioning

confidence: 84%

1,25 Dihydroxyvitamin-D3 Attenuates the Confluence-Dependent Differences in the Osteoblast Characteristic Proteins Alkaline Phosphatase, Procollagen I Peptide, and Osteocalcin

Siggelkow

Schulz²,

Kaesler³

et al. 1999

Calcif Tissue Int

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In the present study a cell culture model of primary human osteoblasts based on degrees of confluence was investigated by measuring basal and 1,25(OH)2D3stimulated levels of the osteoblast characteristic proteins alkaline phosphatase (AP), procollagen I-peptide (PICP), and osteocalcin (OC), as well as the corresponding gene expression. Primary osteoblast-like cell cultures from seven donors were treated in the second passage with 1,25(OH)2D3 (5 x 10(-8) M for 48 hours) and investigated at four stages of confluence (stage I 50%, stage II 75%, stage III 100%, and stage IV 7 days postconfluence). In untreated cultures passing through the different stages of confluence, we saw a 1.8-fold increase of AP activity, a 2. 3-fold increase of OC secretion, but a decrease of PICP levels to 0. 36-fold. Gene expression showed only minor variation between the different confluence stages. 1,25(OH)2D3 did not significantly affect PICP production. Alkaline phosphatase protein was stimulated during proliferation until confluence, with no effect thereafter. Surprisingly, OC secretion and mRNA expression were stimulated in all four stages to the same absolute level independent of basal values. We conclude that our results correspond to other studies showing differentiation-stage dependent changes of basal levels of osteoblast-specific proteins. However, 1,25(OH)2D3 stimulation decreased the confluence-dependent difference for AP and abolished it for osteocalcin, thus leading to a more differentiated phenotype of the osteoblast. Therefore, 1,25(OH)2D3 stimulation might improve the reproducibility of results obtained at different confluence stages from cultures of clinical samples.

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Section: Discussionmentioning

confidence: 84%

1,25 Dihydroxyvitamin-D3 Attenuates the Confluence-Dependent Differences in the Osteoblast Characteristic Proteins Alkaline Phosphatase, Procollagen I Peptide, and Osteocalcin

Siggelkow

Schulz²,

Kaesler³

et al. 1999

Calcif Tissue Int

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show abstract

“…15d-PGJ 2 is derived from PGD 2 and involves the sequential conversion of PGD 2 , PGI 2 , D 12 -PGJ 2 , and 15d-PGJ 2 (Fukushima, 1992). Several proteins have been identified which are induced by cyclopentenone PGs, such as heat shock proteins (Santoro et al, 1989), g-glutamylcysteine synthetase (Ohno et al, 1990), collagen (Tasaki et al, 1991), gadd 45 (Ohtani-Fujita et al, 1998, and heme oxygenase (Koizumi et al, 1995). Although their intracellular receptor has not been described, 15d-PGJ 2 was shown to be a high-affinity ligand for peroxisome proliferator-activated receptor g (PPARg) (Kliewer et al, 1995).…”

Section: Introductionmentioning

confidence: 98%

Thiol antioxidant and thiol-reducing agents attenuate 15-deoxy-Δ12,14-prostaglandin J2-induced heme oxygenase-1 expression

Liu

Tsai²,

Lin³

et al. 2004

Life Sciences

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“…With regard to PGD 2 , PGD 2 reportedly induces collagen synthesis during calcification of osteoblasts [17]. In addition, it has recently been demonstrated that PGD 2 modulates osteoblast function and bone anabolism [18].…”

Section: Introductionmentioning

confidence: 99%

PGD2 stimulates osteoprotegerin synthesis via AMP-activated protein kinase in osteoblasts: Regulation of ERK and SAPK/JNK

Kainuma

Tokuda

Kuroyanagi

et al. 2015

Prostaglandins, Leukotrienes and Essential Fatty Acids

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Prostaglandin D2 metabolite stimulates collagen synthesis by human osteoblasts during calcification

Cited by 45 publications

References 14 publications

1,25 Dihydroxyvitamin-D3 Attenuates the Confluence-Dependent Differences in the Osteoblast Characteristic Proteins Alkaline Phosphatase, Procollagen I Peptide, and Osteocalcin

1,25 Dihydroxyvitamin-D3 Attenuates the Confluence-Dependent Differences in the Osteoblast Characteristic Proteins Alkaline Phosphatase, Procollagen I Peptide, and Osteocalcin

Thiol antioxidant and thiol-reducing agents attenuate 15-deoxy-Δ12,14-prostaglandin J2-induced heme oxygenase-1 expression

PGD2 stimulates osteoprotegerin synthesis via AMP-activated protein kinase in osteoblasts: Regulation of ERK and SAPK/JNK

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