Prostacyclin expression by a continuous human cell line derived from vascular endothelium

Abstract

Prostacyclin is primarily an endothelial cell product. It contributes to the important role of endothelium in maintaining the fluidity of blood by inhibiting platelet aggregation and by promoting vasodilation. Endothelial cells in culture tend to senesce, and the level of prostacyclin expression decreases. A permanent human cell line, EA.hy 926, derived from a fusion of primary endothelial cells with cells of a less differentiated line, has been found to sustain basal and stimulated levels of prostacyclin synthesis.

Stability of the thrombin-thrombomodulin complex on the surface of endothelial cells from human saphenous vein or from the cell line EA.hy 926

“…Activation of protein C on the surface of EA.hy 926-cell-line monolayers The EA.hy 926 cell line has been established through the fusion of human umbilical-vein endothelial cells with the permanent cell line A 549 (Edgell et al, 1983). It has been shown to possess various characteristics of human endothelial cells, such as the synthesis of von Willebrand factor, prostaglandin I2 and thrombomodulin (Suggs et al, 1986). The presence of thrombinthrombomodulin complexes on the surface of these cells was monitored after a short exposure to thrombin, followed by washing, exactly as for HSVEC.…”

Stimulation‐dependent recruitment of cytosolic phospholipase A2‐α to EA.hy.926 endothelial cell membranes leads to calcium‐independent association

“…At concentrations below 200 n m the protein was largely cytosolic, whereas it was completely membrane‐associated at physiologically elevated calcium concentrations of 800 n m and above. In resting endothelial cells, the basal levels of arachidonic acid release and prostacyclin production [32] imply that a pool of cPLA 2 ‐α is constitutively membrane‐associated and catalytically active. Not surprisingly therefore a small proportion of cPLA 2 ‐α was found to be associated with a nonstimulated membrane fraction.…”

Size‐Dependent Cytotoxicity of Monodisperse Silica Nanoparticles in Human Endothelial Cells

“…The human endothelium cells (EAHY926, a hybrid of human umbilical vein endothelial cells26) were seeded in 96‐well plates (66 000 cells per well) in medium supplemented with fetal calf serum (10%), penicillin (100 U mL −1 ), streptomycin (100 µg mL −1 ), l‐glutamine (2 m M ), and fungizone (1.25 µg mL −1 ). After 24 h, pre‐confluent cells were washed twice with DMEM (without serum or antibiotics) and further incubated for 24 h in DMEM (200 µL) containing different nanoparticles at different concentrations.…”