Prospero maintains the mitotic potential of glial precursors enabling them to respond to neurons

Griffiths, Rachel; Hidalgo, Alicia

doi:10.1038/sj.emboj.7600258

Cited by 75 publications

(147 citation statements)

References 55 publications

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“…This is contrary to published data on the development and differentiation of LG, which have been postulated to depend on an intimate interaction with longitudinal axons (Griffiths and Hidalgo, 2004;Griffiths et al, 2007). Nevertheless, LG play an important role in the navigation and fasciculation of longitudinal axons (Hidalgo et al, 1995).…”

Section: Discussioncontrasting

confidence: 55%

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Netrins guide migration of distinct glial cells in the Drosophila embryo

et al. 2010

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SUMMARYDevelopment of the nervous system and establishment of complex neuronal networks require the concerted activity of different signalling events and guidance cues, which include Netrins and their receptors. In Drosophila, two Netrins are expressed during embryogenesis by cells of the ventral midline and serve as attractant or repellent cues for navigating axons. We asked whether glial cells, which are also motile, are guided by similar cues to axons, and analysed the influence of Netrins and their receptors on glial cell migration during embryonic development. We show that in Netrin mutants, two distinct populations of glial cells are affected: longitudinal glia (LG) fail to migrate medially in the early stages of neurogenesis, whereas distinct embryonic peripheral glia (ePG) do not properly migrate laterally into the periphery. We further show that early Netrin-dependent guidance of LG requires expression of the receptor Frazzled (Fra) already in the precursor cell. At these early stages, Netrins are not yet expressed by cells of the ventral midline and we provide evidence for a novel Netrin source within the neurogenic region that includes neuroblasts. Later in development, most ePG transiently express uncoordinated 5 (unc5) during their migratory phase. In unc5 mutants, however, two of these cells in particular exhibit defective migration and stall in, or close to, the central nervous system. Both phenotypes are reversible in cell-specific rescue experiments, indicating that Netrin-mediated signalling via Fra (in LG) or Unc5 (in ePG) is a cellautonomous effect.

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Section: Discussioncontrasting

confidence: 55%

“…In wildtype stage 16 embryos, Pros is differentially expressed in six anterior LG ( Fig. 2D) (Doe et al, 1991;Griffiths and Hidalgo, 2004;Beckervordersandforth et al, 2008). In NetAB ⌬ mutants, on average six LG also expressed Pros (Fig.…”

Section: Research Articlementioning

confidence: 95%

Netrins guide migration of distinct glial cells in the Drosophila embryo

et al. 2010

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“…In addition to the internal clock, extracellular signals may be at work and may control the fine-tuning of proliferation (four to eight cells per lineage). A role of cell-cell interactions in the control of glial proliferation has also been observed in the fly embryo (Griffiths and Hidalgo, 2004).…”

Section: Discussionmentioning

confidence: 89%

Time-lapse and cell ablation reveal the role of cell interactions in fly glia migration and proliferation

et al. 2004

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Migration and proliferation have been mostly explored in culture systems or fixed preparations. We present a simple genetic model, the chains of glia moving along fly wing nerves, to follow such dynamic processes by time-lapse in the whole animal. We show that glia undergo extensive cytoskeleton and mitotic apparatus rearrangements during division and migration. Single cell labelling identifies different glia: pioneers with high filopodial, exploratory, activity and, less active followers. In combination with time-lapse, altering this cellular environment by genetic means or cell ablation has allowed to us define the role of specific cell-cell interactions. First, neurone-glia interactions are not necessary for glia motility but do affect the direction of migration. Second, repulsive interactions between glia control the extent of movement. Finally, autonomous cues control proliferation.

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“…In Drosophila, prospero is a cell-fate determinant in the asymmetric divisions of neuroblasts (Doe et al, 1991;Vaessin et al, 1991) and is required to maintain the mitotic potential of glial cells (Griffiths and Hidalgo, 2004). Prospero is also necessary in the eye for distinguishing the cell fates among color photoreceptors (Cook, 2003).…”

Section: Introductionmentioning

confidence: 99%

Prox1 expression patterns in the developing and adult murine brain

Lavado

Oliver

2006

Developmental Dynamics

142

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Prox1, a homeobox gene related to the Drosophila gene prospero, is necessary for retina, lens, liver, pancreas, and lymphatics development. However, not much is yet known about Prox1 expression during central nervous system development. Here we provide a detailed analysis of Prox1 mRNA and protein expression during prenatal and postnatal murine brain development. Prenatally, Prox1 is expressed in the subventricular zone or in early differentiating regions of the brain. At these stages, Prox1 mRNA, but not Prox1 protein, was also detected in several regions of the prethalamus and hypothalamus. At an early postnatal stage, Prox1 expression is mainly detected in several nuclei of the thalamus, the cerebellum, and the hippocampus. In adulthood, Prox1 expression remains only in the hippocampus and cerebellum. These complex patterns of expression suggest that Prox1 activity is differentially required during brain development and adulthood. Developmental Dynamics 236:518 -524, 2007.

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Prospero maintains the mitotic potential of glial precursors enabling them to respond to neurons

Cited by 75 publications

References 55 publications

Netrins guide migration of distinct glial cells in the Drosophila embryo

Netrins guide migration of distinct glial cells in the Drosophila embryo

Time-lapse and cell ablation reveal the role of cell interactions in fly glia migration and proliferation

Prox1 expression patterns in the developing and adult murine brain

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