Prospects of polysaccharide aerogels as modern advanced food materials

Mikkonen, Kirsi S.; Parikka, Kirsti; Ghafar, Abdul; Tenkanen, Maija

doi:10.1016/j.tifs.2013.10.003

Cited by 142 publications

(90 citation statements)

References 66 publications

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“…The solution was treated with the enzymes CgRaOx (3.3 g/mg substrate), HRP (0.05 U/mg substrate), and catalase (11.2 U/mg substrate) and then stirred for 18 to 66 h at room temperature. HRP and catalase were added to potentially activate the enzyme similarly as in galactose oxidase (27,37). High-dosage experiments were conducted to increase the conversion of raffinose to the product.…”

Section: Methodsmentioning

confidence: 99%

A Novel Colletotrichum graminicola Raffinose Oxidase in the AA5 Family

Andberg

Mollerup

Parikka

et al. 2017

Appl Environ Microbiol

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We describe here the identification and characterization of a copper radical oxidase from auxiliary activities family 5 (AA5_2) that was distinguished by showing preferential activity toward raffinose. Despite the biotechnological potential of carbohydrate oxidases from family AA5, very few members have been characterized. The gene encoding raffinose oxidase from Colletotrichum graminicola (CgRaOx; EC 1.1.3.Ϫ) was identified utilizing a bioinformatics approach based on the known modular structure of a characterized AA5_2 galactose oxidase. CgRaOx was expressed in Pichia pastoris, and the purified enzyme displayed the highest activity on the trisaccharide raffinose, whereas the activity on the disaccharide melibiose was three times lower and more than ten times lower activity was detected on D-galactose at a 300 mM substrate concentration. Thus, the substrate preference of CgRaOx was distinguished clearly from the substrate preferences of the known galactose oxidases. The site of oxidation for raffinose was studied by 1 H nuclear magnetic resonance and mass spectrometry, and we confirmed that the hydroxyl group at the C-6 position was oxidized to an aldehyde and that in addition uronic acid was produced as a side product. A new electrospray ionization mass spectrometry method for the identification of C-6 oxidized products was developed, and the formation mechanism of the uronic acid was studied. CgRaOx presented a novel activity pattern in the AA5 family.IMPORTANCE Currently, there are only a few characterized members of the CAZy AA5 protein family. These enzymes are interesting from an application point of view because of their ability to utilize the cheap and abundant oxidant O 2 without the requirement of complex cofactors such as FAD or NAD(P). Here, we present the identification and characterization of a novel AA5 member from Colletotrichum graminicola. As discussed in the present study, the bioinformatics approach using the modular structure of galactose oxidase was successful in finding a C-6 hydroxyl carbohydrate oxidase having substrate preference for the trisaccharide raffinose. By the discovery of this activity, the diversity of the CAZy AA5 family is increasing.KEYWORDS CAZy AA5, galactose oxidase, nuclear magnetic resonance, NMR, carbohydrate, EC 1.1.3.Ϫ E nzymes that cleave or form oligo-and polysaccharides are classified in the sequence-based carbohydrate-active enzyme database (CAZy [http://www.cazy .org]). The sequences are linked to information about functionality (e.g., substrate specificity) and three-dimensional structure. The CAZy database was recently expanded to account for redox enzymes, leading to the auxiliary activities (AA) group. Currently, 13 AA families exist, comprising lignolytic oxidoreductases (families AA1, -2, -4, and -6), lytic polysaccharide monooxygenases (families AA9, -10, -11, and -13), carbohydrate

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Section: Methodsmentioning

confidence: 99%

A Novel Colletotrichum graminicola Raffinose Oxidase in the AA5 Family

Andberg

Mollerup

Parikka

et al. 2017

Appl Environ Microbiol

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“…Polysaccharide-based aerogels are new and sustainable materials, with a great deal of biocompatibility and abundance, that can be a suitable alternative to silica aerogels for some biomedical applications (i.e. in drug delivery, pharmaceutical, and food industry) demanding biodegradability [69]. Indeed, Kistler has proposed these aerogels in early 1930's with other aerogels by giving this statement: ''we see no reason why this list may not be extended indefinitely'' [141].…”

Section: Bio-based Aerogelsmentioning

confidence: 99%

“…In this regard, polysaccharide-based aerogels due to sustainability, biodegradability, edibility, safety and low cost have been envisaged as a source of energy or nutritional fibers or can be considered as high water absorbent food packaging materials [69], food microencapsulation and food carrier materials [29].…”

Section: Other Life Science and Personal Care Applications Of Aerogelsmentioning

confidence: 99%

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Synthesis and biomedical applications of aerogels: Possibilities and challenges

Maleki

Durães

García‐González

et al. 2016

Advances in Colloid and Interface Science

295

192

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“…Recently, research efforts have focused on the development of materials from environmentally compatible renewable resources. The use of polysaccharides as aerogel matrices presents a great potential for developing bio-based materials (García-González, Alnaief, & Smirnova, 2011;Mikkonen, Parikka, Ghafar, & Tenkanen, 2013). Some native polysaccharides have already been studied in terms of aerogel formation: for example, cellulose-based aerogels (Fischer, Rigacci, Pirard, Berthon-Fabry, & Achard, 2006;Sehaqui, Zhou, & Berglund, 2011) including cellulose nanocomposites (Cai et al, 2012) and alginate aerogels (Quignard, Valentin, & Di Renzo, 2008).…”

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confidence: 99%