Prospects of chitinase in sustainable farming and modern biotechnology: an update on recent progress and challenges

Sharma, Ajay K.; Arya, Shailendra Kumar; Singh, Jatinder; Kapoor, Bhupinder; Bhatti, Jasvinder Singh; Suttee, Ashish; Singh, Gursharan

doi:10.1080/02648725.2023.2183593

Cited by 11 publications

(4 citation statements)

References 73 publications

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“…Despite its role in plant growth, development, and immunity, and its great potential in biocontrol [17][18][19], Chitinase did not show biostimulant effects on the parameters we measured either alone or in combination with other enzymes/Tramesan, since a decrease in TPC, protein, and AsA content was observed.…”

Section: Discussionmentioning

confidence: 78%

“…Aside from protein hydrolysate, to the best of our knowledge, to date, enzymes have never been tested for their potentiality as elicitors of plant growth and resilience. Therefore, in the attempt to identify new and effective BSs, here, we investigated the suitability of two plastid-based proteins usually used for lignocellulosic bioconversion into fermentable sugars, namely a thermophilic Xylanase from Alicyclobacillus acidocaldarius and a hyperthermophilic β-Glucosidase from Pyrococcus furiosus [16], and a Chitinase, known as a biocontrol enzyme being able to inhibit or kill fungi and insects [17][18][19], as biostimulants on lettuce plants (Lactuca sativa L.). As is known, these enzymes are being used in various industrial application ranging from food and feed processing to pharmaceuticals [20].…”

Section: Introductionmentioning

confidence: 99%

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Enzyme-Based Biostimulants Influence Physiological and Biochemical Responses of Lactuca sativa L.

Tamburino,

Docimo,

Sannino

et al. 2023

Biomolecules

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Biostimulants (BSs) are natural materials (i.e., organic or inorganic compounds, and/or microorganisms) having beneficial effects on plant growth and productivity, and able to improve resilience/tolerance to biotic and abiotic stresses. Therefore, they represent an innovative alternative to the phyto- and agrochemicals, being environmentally friendly and a valuable tool to cope with extreme climate conditions. The objective of this study was to investigate the effects of several biomolecules (i.e., Xylanase, β-Glucosidase, Chitinase, and Tramesan), alone or in combinations, on lettuce plant growth and quality. With this aim, the influence of these biomolecules on biomass, pigment content, and antioxidant properties in treated plants were investigated. Our results showed that Xylanase and, to a lesser extent, β-Glucosidase, have potentially biostimulant activity for lettuce cultivation, positively influencing carotenoids, total polyphenols, and ascorbic acid contents; similar effects were found with respect to antioxidative properties. Furthermore, the effect of the more promising molecules (Xylanase and β-Glucosidase) was also evaluated in kiwifruit cultured cells to test their putative role as sustainable input for plant cell biofactories. The absence of phytotoxic effects of both molecules at low doses (0.1 and 0.01 µM), and the significantly enhanced cell biomass growth, indicates a positive impact on kiwifruit cells.

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Section: Discussionmentioning

confidence: 78%

Section: Introductionmentioning

confidence: 99%

Enzyme-Based Biostimulants Influence Physiological and Biochemical Responses of Lactuca sativa L.

Tamburino,

Docimo,

Sannino

et al. 2023

Biomolecules

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“…Biological control using microorganisms that produce chitinases has favorable effects against many post-harvest fungal pathogens [ 68 ]. The application of chitinases in food preservation is promising, as they can degrade the cell wall of contagious fungi—one of the main problems in terms of food deterioration—and prevent the germination process of fungal spores, thus helping to reduce food decomposition and degradation [ 69 ].…”

Section: Resultsmentioning

confidence: 99%

Activity of a Recombinant Chitinase of the Atta sexdens Ant on Different Forms of Chitin and Its Fungicidal Effect against Lasiodiplodia theobromae

Correa,

Facchinatto,

Habitzreuter

et al. 2024

Polymers

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This study evaluates the activity of a recombinant chitinase from the leaf-cutting ant Atta sexdens (AsChtII-C4B1) against colloidal and solid α- and β-chitin substrates. 1H NMR analyses of the reaction media showed the formation of N-acetylglucosamine (GlcNAc) as the hydrolysis product. Viscometry analyses revealed a reduction in the viscosity of chitin solutions, indicating that the enzyme decreases their molecular masses. Both solid state 13C NMR and XRD analyses showed minor differences in chitin crystallinity pre- and post-reaction, indicative of partial hydrolysis under the studied conditions, resulting in the formation of GlcNAc and a reduction in molecular mass. However, the enzyme was unable to completely degrade the chitin samples, as they retained most of their solid-state structure. It was also observed that the enzyme acts progressively and with a greater activity on α-chitin than on β-chitin. AsChtII-C4B1 significantly changed the hyphae of the phytopathogenic fungus Lasiodiplodia theobromae, hindering its growth in both solid and liquid media and reducing its dry biomass by approximately 61%. The results demonstrate that AsChtII-C4B1 could be applied as an agent for the bioproduction of chitin derivatives and as a potential antifungal agent.

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“…Chitinase belongs to the glycosyl hydrolase family that hydrolyzes the glycosidic bond of chitin and has different molecular sizes that vary in a wide range of living systems such as fungi, bacteria, yeasts, plants, actinomycetes, arthropods, and humans [73,74]. Plant chitinases are crucial for embryo development, ethylene production, and resilience against cold, drought, and high salt stress, ensuring survival [75].…”

Section: Chitinasementioning

confidence: 99%

Role of rhizospheric microbial enzymes in plant growth promotion, antagonism, and sustainable agriculture: A review

Galani,

Chauhan,

Gohel

2024

Trends Hortic.

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Plant growth-promoting rhizobacteria (PGPR) offer eco-friendly alternatives to chemical fertilizers, promoting sustainable agriculture by enhancing soil fertility, reducing pathogens, and aiding in stress resistance. In agriculture, they play a crucial role in plant growth promotion through the production of agroactive compounds and extracellular enzymes to promote plant health and protection against phytopathogens. In the rhizosphere, diverse microbial interactions, including those with bacteria and fungi, influence plant health by production of antimicrobial compounds. The antagonism displayed by rhizobacteria plays a crucial role in shaping microbial communities and has potential applications in developing a natural and environmentally friendly approach to pest control. The rhizospheric microbes showcase their ecological importance and potential for biotechnological applications in the context of plant-microbe interactions. The extracellular enzymes produced by rhizospheric microbes like amylases, chitinases, glucanases, cellulases, proteases, and ACC deaminase contribute to plant processes and stress response emphasizing their importance in sustainable agriculture. Moreover, this review highlights the new paradigm including artificial intelligence (AI) in sustainable horticulture and agriculture as a harmonious interaction between ecological networks for promoting soil health and microbial diversity that leads to a more robust and self-regulating agricultural system for protecting the environment in the future. Overall, this review emphasizes microbial interactions and the role of rhizospheric microbial extracellular enzymes which is crucial for developing eco-friendly approaches to enhance crop production and soil health.

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Prospects of chitinase in sustainable farming and modern biotechnology: an update on recent progress and challenges

Cited by 11 publications

References 73 publications

Enzyme-Based Biostimulants Influence Physiological and Biochemical Responses of Lactuca sativa L.

Enzyme-Based Biostimulants Influence Physiological and Biochemical Responses of Lactuca sativa L.

Activity of a Recombinant Chitinase of the Atta sexdens Ant on Different Forms of Chitin and Its Fungicidal Effect against Lasiodiplodia theobromae

Role of rhizospheric microbial enzymes in plant growth promotion, antagonism, and sustainable agriculture: A review

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