Prospects for using native and recombinant rhamnolipid producers for microbially enhanced oil recovery

Rhamnolipid production from waste cooking oil using Pseudomonas SWP-4

“…23 In light of the present emphasis placed on the management and recycling of the 24 wastes, developing the practical processes to transform WCO into products has been a 25 great concern. WCO is a kind of cheap and available resource that can be used for 26 biodiesel production, which is a very hot research topic in last decade. However, 27 biodiesel production is quite heavily dependent on fossil fuel price.…”

Microbially enhanced oil recovery from miniature model columns through stimulation of indigenous microflora with nitrate

“…Length and inner diameter of the 30 mL syringe barrels were 9.6 and 2 cm, whereas those of the 20 mL syringe barrels were 8.5 and 1.8 cm. Columns were assembled using 50e70 mesh quartz sand (SigmaeAldrich) as described by Kryachko et al (2013).…”

“…Following an injection of EO into SPW-saturated columns (Kryachko et al, 2013), residual water saturation (S wr ) was determined. On average, S wr was 1.5 ± 0.4 mL for 30 mL columns and 0.7 ± 0.1 mL for 20 mL columns (Table 1).…”

“…Oil was separated from water by adding a known volume of dichloromethane (DCM). The fraction of oil in the combined organic phase was then determined by absorbance at 600 nm (Kryachko et al, 2013). Average water flood-driven production of oil (Vo/PV) and water (Vw/PV) from EO-saturated 30-mL columns is shown in Fig.…”

“…2), in an incubator in the anaerobic hood at 30 C for repeated incubations which lasted on average 58 days (Supplementary Resource 2). At the end of an incubation period, the vials were removed and the amounts of produced oil and water, as well as the concentrations of N 2 and CO 2 , were measured as described by Kryachko et al (2013). Afterwards, 1 PV of CSBK-nitrate or CSBK were injected into the columns (Supplementary Resources 1 and 2).…”